Heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device and application thereof
A technology of capillary electrophoresis and heart cutting, which is applied in the direction of measuring devices, scientific instruments, and material analysis through electromagnetic means, to achieve high sensitivity, eliminate dead volume, and avoid loss and dilution effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0023] Example 1 Construction of heart-cutting two-dimensional capillary electrophoresis on-column electrochemical device
[0024] A specific heart-cutting 2D capillary electrophoresis on-column electrochemical setup such as figure 1 shown. At 1.0cm away from the outlet end of capillary 2 (50μm.d., 375μm o.d., Hebei Yongnian Ruifeng Chromatographic Devices Co., Ltd.), remove a section of polyimide coating on one side of the capillary, and place the part of the capillary that has been removed in a hydrofluoric It is etched into a porous shape in the acid solution, and a layer of cellulose acetate pulp is evenly coated on the place. After solidification, a field isolation joint that only passes current and no solution leakage is formed; the field isolation joint is placed in polytetrafluoroethylene In the cell body 4, there are capillary tube access openings and electrode sockets in the cell body 4; the capillary tube 2 and the above-mentioned treated field isolation joint are ...
Embodiment 2
[0025] Example 2 Application of heart-cutting two-dimensional capillary electrophoresis to separation and detection of 9 drug compounds in blood samples with a column electrochemical device. Experimental conditions:
[0026] The nine drug compounds are: propranolol, acebutolol, metoprolol, propranolol, labetalol, timolol, pindolol, atenolol, and nadolol Er (USA, Sigma Company), sodium lauryl sulfate, glacial acetic acid, anhydrous sodium acetate, and acetonitrile were all analytically pure (Shanghai Chemical Reagent Company), other reagents were analytically pure, and the experimental water was double distilled water.
[0027] The first dimension separation adopts capillary zone electrophoresis, the separation buffer is 90mmol / L sodium acetate, and the pH is adjusted to 4.3 with concentrated acetic acid; the second dimension separation adopts micellar electrokinetic capillary chromatography, and the separation buffer is 15mmol / L sodium acetate+30mmol / L sodium lauryl sulfate, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com