Artificially cultured black termitornyces albuminosus berk heim entity and culture method thereof
A technology of artificial cultivation and cultivation methods, applied in the fields of botanical equipment and methods, fertilizer mixtures, horticulture, etc., can solve the problems that bacteria are difficult to meet the huge market demand, there is no annual cultivation of black chickens, and large-scale promotion is affected. It has the effect of decomposing digestion and absorption, relieving gastrointestinal spasm, and inhibiting the growth of rice blast bacteria
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Embodiment 1
[0022] 1. Preparation of mother species:
[0023] (1) Selected black chicken collected from the wild Bacteria obtained pure strains through tissue and spore separation methods, and the pure strains were purified and selected for multiple generations to obtain a stable growth rate on CPDA medium, thick and thick moss mycelium, neat and well-developed aerial mycelia, white color, and strong growth. swift black chicken The original mother species of bacteria;
[0024] (2) Prepare and expand the female species, add CPDA medium into test tubes or other glass containers, sterilize and cool down and insert black chicken Cultivate the original mother species of the fungus at a temperature of 20-28°C. After 7-10 days of cultivation, the mycelium will cover the surface of the medium and become a mature mother species, which can be used to reproduce the original species;
[0025] 2. Preparation of original species and cultivated species:
[0026] (1) The medium raw material is com...
Embodiment 2
[0036] 1. The preparation of the mother species is the same as in Example 1.
[0037] 2. Preparation of original species and cultivated species:
[0038] (1) The medium raw material is composed of (weight percentage, the same below)
[0039] 80% miscellaneous sawdust, 18% wheat bran, 1% sucrose, and 1% gypsum powder form the solid material, add water to make the water content reach 62%-68%, and adjust the pH value to 7.5-8.5 with lime powder to obtain the original seed or cultivated medium;
[0040] (2) Same as Example 1.
[0041] 3. Black chicken Annual cultivation of bacteria:
[0042] (1) Preparation of medium:
[0043] 60% miscellaneous wood sawdust, 10% cottonseed hulls, 20% corncobs, 8% bran, 1% gypsum, and 1% sugar form a solid material. Add water to make the water content in the medium reach 62% to 68%. Use lime powder Adjust the pH value to 7.5-8.5 to obtain a cultivation medium;
[0044] The following steps are the same as in Example 1.
Embodiment 3
[0046] 1. The preparation of the mother species is the same as in Example 1.
[0047] 2. Preparation of original species and cultivated species:
[0048] (1) The medium raw material is composed of (weight percentage, the same below)
[0049] 70% of cottonseed hulls, 20% of sawdust, 8% of bran, 1% of sucrose, and 1% of gypsum constitute the solid material, add water to make the water content reach 62%-68%, and adjust the pH value of lime powder to 7.5-8.5 to obtain the original species or cultivation medium;
[0050] (2) Same as Example 1.
[0051] 3. Black chicken Annual cultivation of bacteria:
[0052] (1) Preparation of medium:
[0053] 68% miscellaneous sawdust, 15% bran, 15% rice bran, 1% gypsum, and 1% sugar constitute the solid material. Add water to make the water content in the culture medium reach 62%-68%, and adjust the pH value to 7.5-8.5 with lime powder. Cultivation medium;
[0054] The following steps are the same as in Example 1.
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