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Emulsion based on stabilizing agent, preparation method and application thereof

A technology of stabilizer and emulsion, applied in the field of stabilizer-based emulsion and its preparation, can solve the problems of skin irritation, affecting wettability, and limiting the application range of emulsion

Inactive Publication Date: 2010-06-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its amphiphilicity also allows it to be adsorbed on the surface of any solid medium, significantly affecting the wettability of the latter, which limits the application range of emulsions to a certain extent, such as surfactants used in cosmetics. skin irritation

Method used

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  • Emulsion based on stabilizing agent, preparation method and application thereof
  • Emulsion based on stabilizing agent, preparation method and application thereof
  • Emulsion based on stabilizing agent, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The experimental materials involved in this embodiment are as follows:

[0032] Ferrous chloride, ferric chloride, and sodium hydroxide were all analytically pure, purchased from China Pharmaceutical Group Shanghai Chemical Reagent Company;

[0033] Polylactic acid (PLA), purchased from Shanghai Bybo Biotechnology Co., Ltd.;

[0034] 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) was purchased from Sigma;

[0035] Polyethyleneimine (PEI) 25000 is purchased from Sigma company;

[0036] soybean oil, commercially available;

[0037] Sulfur hexafluoride (SF 6 ) gas was purchased from CNNC Honghua Special Gas Co., Ltd.;

[0038] African green monkey kidney cell line COS-7 cells were purchased from ATCC;

[0039] RPMI-1640 medium and serum were purchased from GIBICO;

[0040] The luciferase reporter plasmid pGL3-control was purchased from Promega, USA;

[0041] The PCS plasmid is disclosed in the publication specification of Chinese invention patent application CN...

Embodiment 2

[0049]The present embodiment adopts the same materials and steps as in Example 1 to prepare a stabilizer, and the method for preparing an emulsion based on the stabilizer specifically includes the following steps:

[0050] Ultrasonic preparation of O / W emulsion using the above stabilizer: Take the solution obtained by dialysis (i.e. the stock solution of PLA magnetic particles), dilute it 5 times, take 900ul to mix with 100ul soybean oil, 25 ° C, 30% ultrasonic intensity, ultrasonic 7 times , every time 5S on, 6S off, get O / W emulsion.

Embodiment 3

[0052] The present embodiment adopts the material identical with embodiment 1 to prepare positively charged stabilizer, and this preparation method specifically comprises the following steps:

[0053] Step 1, take polylactic acid, FeCl 3 and FeCl 2 , preparing magnetic nanoparticles by chemical co-precipitation method;

[0054] Weigh 730mg FeCl 3 and 360mg FeCl 2 Dissolve in 5ml degassed water to obtain iron salt solution; then take 2.8g PLA and dissolve it in 2ml degassed water to obtain a solution, mix the solution with iron salt solution to obtain a mixed solution; place the mixed solution in an ice-water bath ( 2-4°C), under the protection of nitrogen, carry out the following operation while stirring: add NaOH (5M) aqueous solution drop by drop, monitor the pH value online with a pH meter, stop the titration when the pH value is 10.5-11, and continue the ice bath for 30 Minutes; under the protection of nitrogen, heat the solution in an oil bath at 85°C for 2 hours to m...

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Abstract

The invention discloses an emulsion based on stabilizing agent, a preparation method and application thereof, belonging to the technical field of biomedicine. Chemical coprecipitation method is carried out on polylactic acid and iron halide to prepare stabilizing agent; or 1-(3 dimethylamino propyl)3 ethyl carbodiimide and polymine are further adopted for modifying to obtain stabilizing agent; stabilizing agent is mixed with water phase and oil phase to prepare emulsion, and emulsion is applied gene vector, magnetic resonance imaging contrast agent or ultrasonic diagnosis contrast agent. The stabilizing agent prepared in the invention has better hydrophily, lipophilicity and magnetism, and has certain electropositivity after modification; the prepared emulsion has magnetism and electropositivity, and a potential to be used as contrast agent for magnetic gene vector and magnetic resonance imaging; the emulsion prepared by supplying sulfur hexafluoride also has a potential to be used ascontrast agent for ultrasonic diagnosis.

Description

technical field [0001] The invention relates to an emulsion in the technical field of biomedicine and its preparation method and application, in particular to a stabilizer-based emulsion and its preparation method and application. Background technique [0002] An emulsion is a heterogeneous mixture of droplets of one liquid phase dispersed in another completely incompatible liquid phase. The mutual incompatibility of the two liquid phases causes the two to generate surface tension where they contact each other. When one liquid phase is dispersed in the other liquid phase in the form of droplets, the contact area between the two increases significantly. , leading to an increase in the chemical potential energy, and the droplets will spontaneously collide and fuse to make up for the lost free energy. Therefore, the emulsion is a thermodynamically unstable system. The preparation of a stable emulsion needs to create a layer of kinetic energy between the droplets. Physical barr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/22C12N15/63
Inventor 徐宇虹郭微
Owner SHANGHAI JIAO TONG UNIV
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