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Method for improving content of resveratrol by converting giant knotweed materials through immobilized enzyme method

A technology of immobilizing enzymes and resveratrol, which is applied in the direction of immobilization on/in organic carriers, fermentation, etc., can solve the problems of difficult control of catalysis time, unrecoverable cost of enzymes, long conversion time, etc., and shorten production cycle, simplify the production process and operation, and improve the effect of product quality

Active Publication Date: 2010-06-16
广东佳洁化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned defects and provide a process for producing high-purity resveratrol from Polygonum cuspidatum using immobilized enzyme technology, which can increase the content of resveratrol in Polygonum cuspidatum material by 10-20 times and avoid similar In the technology, the conversion time is too long, the catalysis time is not easy to control, and the enzyme cannot be recovered after the reaction is completed, resulting in increased costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Production of immobilized enzyme:

[0022] a) Purchase β-glucosidase with an activity greater than or equal to 5000U / g, and then prepare an enzyme solution with a concentration of 20% for use.

[0023] b) Weigh sodium alginate and distilled water according to the weight ratio of sodium alginate and distilled water at 2-5:100, add sodium alginate to distilled water at room temperature and stir evenly, let it stand for swelling for 20 minutes, and then swell at 80-90°C Heat it with water to make it fully dissolved. The color is uniform and there is no condensed granular as the standard. After completion, cool to below 45°C.

[0024] c) Pour the prepared enzyme solution into the sodium alginate gel, stir well until uniform, and then use a syringe or a cross-flow pump to drop 2.5-3% CaCl at a constant speed through a No. 7 needle 2 Gel particles are formed in the solution, adjust the flow rate so that the diameter of the formed particles is between 2-4 mm, filter out th...

Embodiment 2

[0034] 1, the making of immobilized enzyme: with embodiment 1

[0035] 2. Preparation of Polygonum cuspidatum crude extract:

[0036] a) Select Polygonum cuspidatum, fully dry in the shade, pulverize through a pulverizer, and pass through a 40-60 mesh sieve.

[0037] b) Ultrasonic-assisted extraction: use 80-95% methanol at a ratio of 1:3 (dry weight of Polygonum cuspidatum: methanol volume, the unit of Polygonum cuspidatum is g, and the unit of methanol is mL) at 30-35 ° C to use ultrasonic treatment on the mixed system, power Control at 80-150W, extract 3 times, each time 1-1.5h, after completion, suction filter and combine the filtrates.

[0038] c) Evaporate the filtrate to 6-9% of the original volume, then add water with 5-10% volume of the original ethanol therein, adjust the pH to 4.0-6.0, and set aside.

[0039] 3. Transformation and fine extraction of Polygonum cuspidatum crude extract:

[0040] d) Add the immobilized enzyme of 5% of the weight of the extract in th...

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Abstract

The invention discloses a method for improving the content of resveratrol by converting giant knotweed materials through an immobilized enzyme method. The method comprises the steps of biologically converting a crude extract of the giant knotweed materials with a specific immobilized enzyme to convert resveratrol analogues therein into the resveratrol in a short time and then obtaining the resveratrol with a high purity by extraction and separation technologies. The method can increase the resveratrol content of the giant knotweed materials by 10 to 20 times, solves the problems of overlong conversion time, difficult control on catalytic time as well as increased cost caused by the fact that the enzyme cannot be recovered after the reaction in the similar techniques, and simultaneously obtains over 95 percent of resveratrol and a side-product archen with a certain purity by adopting the separation and refining techniques such as chromatography, crystallization and the like.

Description

Technical field: [0001] The invention relates to the fields of biotransformation technology and extraction of natural active substances in plants, and in particular provides a process for purifying resveratrol by using immobilized enzymes to convert knotweed materials. Background technique: [0002] Polygonum cuspidatum Sie bet Zucc is a small shrub of Polygonum genus Polygonum, which is a traditional Chinese medicine in my country, which is rich in resveratrol (3,4',5-trihydroxy-trans-stilbene, Resvertrol). Resveratrol is a non-flavonoid polyphenol with moderate activity. Widely present in natural foods or medicines such as grapes and peanuts, it is a plant physiologically active substance with clear physiological functions, and has the effects of anticancer and treating cardiovascular diseases. [0003] The search found that domestic and foreign literature and patents on the "extraction of resveratrol by biotransformation" have been reported. For example, the process of ...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/04C12P7/22
Inventor 曹庸李赟徐勇
Owner 广东佳洁化妆品有限公司
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