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Preparation method of duck hepatitis antibody

A duck hepatitis and antibody technology, applied in the field of immunology, can solve the problems of complicated serum manufacturing procedures, difficulty in meeting production needs, and high cost, and achieve the effects of convenient storage and transportation, convenient material collection, and high specificity

Active Publication Date: 2010-05-12
烟台爱士津动物保健品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the effect of passive immunization on ducklings with duck hyperimmune serum or convalescent serum is good, the serum production procedures are complicated, the yield is low, and the cost is high, so it is difficult to meet the production needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 duck hepatitis antibody

[0025] Healthy laying hens were fed with poplar bark lipid, and each chicken was fed with 0.08g per day, once a day, for one month continuously.

[0026] Duck hepatitis virus strain AVZ111 was used to inoculate SPF chicken embryos, collected 60-96h dead embryo bodies, chorion and allantoic fluid, mixed and mashed, frozen and thawed three times, centrifuged, supernatant was taken, and inactivated by adding 0.1% formaldehyde. Then it was fully emulsified with oil adjuvant to make an oil emulsion inactivated vaccine, which was used to immunize healthy laying hens fed for one month with poplar bark lipid (purchased from Ju County Forestry Chemical Factory, Shandong Province). For the first immunization, inject 0.5ml attenuated vaccine into the breast muscle of each chicken, perform the second immunization 14 days later, inject 1.0ml oil emulsion inactivated vaccine into the breast muscle of each chicken, and perform ...

Embodiment 2

[0032] Except that the feeding amount of poplar bark lipid was 0.1 g per chicken per day, and the final concentration of poloxamer 188 added to the mixture of yolk and saline was 2.0%, the rest of the steps were the same as in Example 1.

[0033] The potency of the prepared duck hepatitis antibody is more than 1:256, the safety is 100%, and the sterility test result is negative.

[0034] Therapeutic dosage: emergency chest intramuscular injection of 2.0ml per duck in the early stage of disease.

[0035] Preventive dosage: Inject 1.0ml / duck into the breast muscle of 3-day-old ducks, and inject 1.0ml / duck into the breast muscle of ducks 10 days later.

Embodiment 3

[0037] Except that the feeding amount of poplar bark lipid was 0.09 g per chicken per day, and the final concentration of poloxamer 188 added to the mixture of egg yolk and saline was 1.5%, the rest of the steps were the same as in Example 1.

[0038] The potency of the prepared duck hepatitis antibody is more than 1:256, the safety is 100%, and the sterility test result is negative.

[0039] Therapeutic dosage: emergency chest intramuscular injection of 2.0ml per duck in the early stage of disease.

[0040] Preventive dosage: Inject 1.0ml / duck into the breast muscle of 3-day-old ducks, and inject 1.0ml / duck into the breast muscle of ducks 10 days later.

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PUM

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Abstract

The invention relates to the field of immunology, in particular a preparation method of duck hepatitis antibody. The preparation method comprises the following steps: (1) feeding healthy laying hens by poplar bark lipoid at a dosage of 0.08 to 0.1g per hen each day; carrying out duck hepatitis inactivated vaccine intensive immunity for healthy laying hens fed by poplar bark lipoid after 20 to 30 days; collecting hen blood serum to measure anti-DHV neutralizing antibody titer; and collecting eggs laid by qualified flocks; and (2) separating egg white under aseptic conditions, mashing yolk and evenly mixing the yolk liquid with aseptic physiological saline to prepare yolk antibody. Compared with the antibody from mammals, antibody prepared through the preparation method has the advantages of convenient material sources, simple separation and purification method, high yield, high specificity and better stability.

Description

technical field [0001] The invention relates to the field of immunology, in particular, the invention relates to a method for preparing duck hepatitis antibody. Background technique [0002] Duck viral hepatitis, which is more prevalent in winter and spring, is an acute contact-transmitted disease of ducks. It is mainly characterized by neurological symptoms such as ataxia, turning in circles, kicking after the spasm of the two feet, and turning the head to the back. The course of the disease is rapid, the onset is sudden, the spread is fast, and the patient often dies within 3 to 4 days after the onset. But it is only an acute infectious disease that is popular in ducklings, so it is also called duckling viral hepatitis, especially ducklings of 1 to 2 weeks are the most susceptible, and there are many diseases. About 57% to 95%, it is a serious disease that threatens the duck industry and cannot be ignored. The key to preventing and treating this disease should be to mak...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02
Inventor 张兴晓丁军涛吕淑荣孔义波郭东王颖
Owner 烟台爱士津动物保健品有限公司
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