Asia I-type aftosa synthetic peptide vaccine

Abstract

The invention provides Asia I-type aftosa synthetic peptide vaccine, and in particular relates to polypeptide or polypeptide polymer thereof used in the vaccine as well as the vaccine containing the polypeptide or the polypeptide polymer thereof and a preparation method thereof. The polypeptide has amino acid sequences shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID NO.3. The Asia I-type aftosa synthetic peptide vaccine carries out chemical synthesis of potential antigen site peptide segments by carrying out sequencing of domestic aftosa epidemic strains to study the variation of the main antigen sites of aftosa and combining with a computer-aided method to carry out aftosa antigen site analytical prediction; and candidate polypeptide antigens are screened out by carrying out large numbers of animal experiments to obtain polypeptide antigens which have high immunoreaction level and can protect animals from the attack of aftosa epidemic strains. The Asia I-type aftosa synthetic peptide vaccine prepared by the polypeptide antigens can effectively cope with the antigen variation of aftosa virus without causing biosafety problems and has easy large-scale synthesis, thereby having a good application prospect.

Description

technical field [0001] The present invention relates to a polypeptide or its polypeptide polymer used for foot-and-mouth disease synthetic peptide vaccines, vaccines containing the polypeptide or its polypeptide polymer and their preparation methods; in particular to a synthetic peptide vaccine for Asian type I foot-and-mouth disease Polypeptide or its polypeptide polymer, vaccines containing the polypeptide or its polypeptide polymer and their preparation methods. Background technique [0002] Foot and mouth disease (FMD) is an acute, highly contagious, febrile infectious disease that occurs in cloven-hoofed animals. Foot-and-mouth disease is caused by foot-and-mouth disease virus, which belongs to the genus of foot-and-mouth disease virus in the family Picornaviridae. It is spherical in shape with a diameter of 30nm and no envelope. Foot-and-mouth disease virus has a simple structure and is composed of single-stranded RNA and protein. It is most abundant in the blister sk...

AI Technical Summary

Problems solved by technology

However, due to the limited amount of gene expression products and the inability to obtain the three-dimensional structure of the antigen, it is difficult to improve the immune efficacy

In 2003, Zheng Zhaoxin et al. concatenated the T-cell epitope and B-cell epitope genes on VP1 and VP4 to develop a genetically engineered vaccine against Asia 1 type foot-and-mouth disease, but the test results showed that it could not reach the target level under virulent attack. 100% protection, at the same time as a vaccine, the post-processing of genetically engineered vaccines is too complicated, the cost is high, and the potency is low

At present, a synthetic peptide vaccine against porcine O-type foot-and-mouth disease virus has been successfully developed, but there is no synthetic peptide vaccine against Asian type-1 foot-and-mouth disease virus in the world, and Asian type-1 foot-and-mouth disease virus is one of the most prevalent foot-and-mouth disease viruses in my country. A synthetic peptide vaccine against Asian type 1 foot-and-mouth disease virus with good efficacy, high safety, stable production process and low cost has become an urgent and important task

Images