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Method for preparing rapeseed peptides by microorganism liquid fermentation

A technology of liquid fermentation and microorganism, which is applied in the field of preparing rapeseed peptide by liquid fermentation of microorganisms, can solve the problems that the content of soluble nitrogen and biological activity need to be further improved, it is difficult to form large-scale industrial production, and the activity of protease is affected, and it is beneficial to environmental protection. , the effect of saving biological enzyme preparations and increasing soluble nitrogen content

Inactive Publication Date: 2010-02-24
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses rapeseed protein isolate as raw material, and the amount of enzyme preparation used in production is large and expensive, resulting in high production costs, and because the anti-nutritional factors in rapeseed meal or rapeseed protein isolate cannot be completely removed, the activity of protease is affected. , the degree of hydrolysis is not high, it is difficult to form a large-scale industrial production
[0004] CN200810243461.6 and CN200810106919.3 disclose methods for modifying rapeseed meal by biological fermentation. Although harmful substances such as glucosinolate (hereinafter referred to as glucosinolate) in rapeseed protein are removed, rapeseed peptide or rapeseed Seed peptide products, but the soluble nitrogen content and biological activity of the product still need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Preparation of Rapeseed Peptide by Liquid Fermentation of Bacillus subtilis (Alkaline Protease Producing)

[0020] (1) Preparation of liquid fermentation medium

[0021] Nutrient solution composition: glucose 2.0g, MnSO 4 1.0g, tap water 1000mL, pH 9.5. Crush the double-low rapeseed meal to a particle size of about 100 μm, mix the rapeseed meal evenly with the nutrient solution at a solid-to-liquid ratio of 1:15 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium. Inside the fermenter.

[0022] (2) Liquid fermentation

[0023] ① Preparation of seed solution:

[0024] Slant medium formula: beef extract 3g, peptone 10g, NaCl 5, agar 20g, tap water 1000mL, pH7.2-7.4.

[0025] Seed medium formula: beef extract 3g, peptone 10, NaCl 5g, tap water 1000mL, pH 7.0.

[0026] From the activated strain culture slope, pick two rings of Bacillus subtilis and inoculate it in the seed medium, seal it with 8 layers of gauze, culture it on ...

Embodiment 2

[0036] Example 2 Preparation of Rapeseed Peptide with Specific Antioxidant Activity by Liquid Fermentation of Bacillus subtilis

[0037] The difference from Example 1 is that (4) purification is carried out as follows:

[0038] Adjust the pH of the rapeseed peptide crude extract to 6.5-7.5, add 10g / L activated carbon, decolorize and debitterize at 50°C for 1h, then centrifuge at a high speed (relative centrifugal force 12000g) to remove impurities, and the supernatant is passed through a desalting column (HiPrep TM26 / 10, flow rate 30mL / min) desalination, spray drying to obtain biologically active rapeseed peptide powder, in which the protein content is 88% (dry basis, N×6.25), the soluble nitrogen content is 92%, and the molecular weight is 1000Da~5000Da peptide Content 62%, glucosinolates were not detected.

[0039] The antioxidant bioactivity indicators of rapeseed peptide are as follows:

[0040] Rapeseed peptide reducing ability: when the product concentration is 1mg / mL,...

Embodiment 3

[0044] Example 3 Preparation of Rapeseed Peptide by Liquid Fermentation of Mucor radiata

[0045] (1) Preparation of liquid fermentation medium

[0046] Nutrient solution composition: glucose 50g, KH 2 PO 4 2g, tap water 1000mL, pH 6.5. Crush the ordinary rapeseed meal to a particle size of about 100 μm, mix it evenly with the rapeseed meal with a nutrient solution at a solid-to-liquid ratio of 1:30 g / mL, and sterilize at 121°C for 30 minutes to obtain a liquid fermentation medium for fermentation inside the tank.

[0047] (2) Liquid fermentation

[0048] ① Preparation of seed solution:

[0049] Slant medium formula: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0050] Plate expansion medium formula: 300g potato, 20g glucose, 20g agar, 1000mL tap water.

[0051] Inoculate the spores of Mucor actinosa on the slant medium and incubate at 28°C for 4 days, then inoculate on the flat plate and carry out expansion culture at 28°C. After 4 days, wash the spores with...

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Abstract

The invention relates to a method for preparing rapeseed peptides by microorganism liquid fermentation, which sufficiently utilizes the functions of hydrolyzing proteins and degrading sulfuric glucoside by microorganism, thereby effectively improving the soluble nitrogen content and the bioactivity of the product. The method for preparing the rapeseed peptides by microorganism liquid fermentationcomprises the following steps: (1) mixing rapeseed meal powder with nutrient solution to prepare liquid fermentation medium; (2) inoculating the microorganism into the fermentation medium to carry outliquid fermentation; (3) carrying out solid-liquid separation on the liquid fermentation medium after fermentation to obtain the crude extract of the rapeseed peptides; and (4) purifying the crude extract to obtain the rapeseed peptides. A strain capable of generating protease is actinomucor elegans, aspergillus usamii, bacillus subtilis, lactobacillus or candida utilis. The invention has simplepreparation process, utilizes the microorganism liquid fermentation to generate a plurality of metabolites and enzymes and sufficiently utilizes the functions of hydrolyzing proteins and degrading sulfuric glucoside by the microorganism, thereby improving the soluble nitrogen content, the bioactivity and the yield of the rapeseed peptides.

Description

technical field [0001] The invention relates to a method for preparing rapeseed peptide by microbial liquid fermentation. Background technique [0002] my country is the country with the largest rapeseed production in the world, with an annual output of nearly 12 million tons. Rapeseed meal is the main by-product of rapeseed oil processing, accounting for 65% of the total rapeseed, which contains 35% to 42% protein. The amino acid composition of rapeseed protein is reasonable, the content of lysine, cystine and methionine is relatively high, which is similar to the amino acid composition model recommended by WHO / FAO, and its nutritional value is equal to or better than that of animal protein. It is an excellent vegetable protein. However, due to the existence of some anti-nutritional factors in rapeseed meal, such as glucosinolates, phytic acid, polyphenols, etc., it is difficult to remove them during processing, so rapeseed meal can only be used in the feed or fertilizer in...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P21/06C12R1/785C12R1/66C12R1/125C12R1/72C12R1/225
Inventor 何荣鞠兴荣袁建王立峰周建新宋佳蒋甜燕
Owner NANJING UNIV OF FINANCE & ECONOMICS
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