Application of pigeon pea stilbene acid in the preparation of antitumor drug

A tumor drug, pigeonpea leaf technology, applied in the field of medicine, to achieve the effect of low toxicity, not easy to produce drug resistance, efficient and rational use, and good anti-tumor activity

Active Publication Date: 2011-02-02
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of pigeonpea leaf supercritical extract and pigeontostilnic acid, including pigeontostilnic acid Ⅰ-Ⅵ, for antitumor drugs has not been reported so far

Method used

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  • Application of pigeon pea stilbene acid in the preparation of antitumor drug
  • Application of pigeon pea stilbene acid in the preparation of antitumor drug
  • Application of pigeon pea stilbene acid in the preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Cells: human breast cancer cells (MCF-7), human lung cancer cells (A549), human gastric cancer cells (SGC-7901), human colon cancer cells (HCT-8), human leukemia cells (CEM), human ovarian cancer cells ( HO-4980), human cervical cancer cells (Hela), human liver cancer cells (HepG2), human glioma cells (SHG-44), mouse solid tumor S180 cells. All were purchased from Shanghai Cell Bank.

[0017] Experimental materials: pigeonpea supercritical extract and pigeontostilnic acid, including pigeontostilnic acid Ⅰ-Ⅵ, prepared in our laboratory; normal saline, Harbin Sanlian Pharmaceutical; paclitaxel, Shanghai Haohua Chemical Co., Ltd.

[0018] method:

[0019] (1) Tumor cells in the exponential growth phase were collected and diluted to 20,000 cells / mL with RPMI1640 culture medium containing 10% fetal bovine serum.

[0020] (2) Add 200 μL of cell suspension to each well of a 96-well plate and place at 37°C, 5% CO 2 Cultivate in the incubator for 24h.

[0021] (3) Dissolve ...

example 2

[0033] S180 ascites subcultured mice were purchased from Heilongjiang Cancer Research Hospital. Kunming mice, weighing 24-25 g, were purchased from the Experimental Animal Center of Heilongjiang Cancer Hospital. Cyclophosphamide was purchased from the Second Affiliated Hospital of Harbin Medical University.

[0034] Methods: Experimental mice were randomly divided into control group (normal saline 200mL / kg), cyclophosphamide (20mg / kg), pigeontostilnic acid I (40mg / kg, 15mg / kg), pigeonpea supercritical extract ( 250mg / kg, 125mg / kg) due to the decomposition of paclitaxel after intragastric administration, cyclophosphamide was selected as the positive control drug for the in vivo test. Select mice with tumor transplantation for 7 days, good tumor growth and obvious abdominal bulge, disinfect the abdominal skin, use a disposable non-toxic blood sampling device to penetrate the abdominal cavity through the abdominal wall, extract ascites, put it into a sterile beaker and dilute it...

example 3

[0039] 1. Using laser confocal microscope to study the DNA morphology of breast cancer MCF-7 cells

[0040]Take MCF-7 cells in the logarithmic growth phase, add 5 μM pigeontostilnic acid, and set a negative blank control. MCF-7 cells were incubated for 48 hours, culture medium was removed, washed twice with cold PBS, PC-3 cells were digested with 0.25% trypsin, and MCF-7 cells were collected by centrifugation at 3000×g, washed twice with cold PBS, and centrifuged at 3000×g 5min to remove PBS. The cells were collected by centrifugation at 3000×g for 5 minutes, and resuspended with cold PBS buffer to adjust the cell density to 1×10 3 / mL, and add 1mL 50μg / mL DAPI, pipette evenly, incubate at room temperature in the dark for 5min, and use a laser confocal microscope to study the DNA morphology of prostate cancer MCF-7 cells.

[0041] result: figure 2 (A) is the blank control MCF-7 cells, figure 2 (B) is the MCF-7 cells added with drugs. In comparison, (B) the nuclear chroma...

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Abstract

The invention relates to a supercritical extract of pigeon pea leaves and the new application of the main component of the extract ---pigeon pea stilbene acid (containing pigeon pea stilbene acids I-VI) in antitumor aspect. After different excipients are added to the pigeon pea stilbene acid, the pigeon pea stilbene acid can be processed into any pharmaceutical dosage form. The supercritical extract of pigeon pea leaves and pigeon pea stilbene acid (containing pigeon pea stilbene acid I-VI) has the characteristics of light toxic and side effect, accurate curative effect, safety, reliability, and the like. Pigeon peas have the advantages of abundant resources, convenient collection, simple extraction process and convenience for industrial production.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the use of cassinoleic acid I in pigeonpea leaves in the preparation of drugs for treating tumors. Background technique [0002] Tumor is one of the most common and serious diseases that directly threaten human life in the world today. In recent years, with the continuous development of molecular oncology and molecular pharmacology and the gradual elucidation of the nature of tumors, the wide application of advanced technologies such as drugs, genetic engineering, combinatorial chemistry, and large-scale rapid screening has accelerated the development of new anti-tumor drugs. At present, the main anti-tumor drugs are: alkylating agents, anti-metabolite drugs, anti-tumor antibiotics, platinum-based anti-tumor drugs, hormones, anti-tumor herbal medicines, used for microtubules and tubulin (such as: paclitaxel, etc.), used In topoisomerase (such as: camptothecin, etc.), inhibit tumor cell ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K127/00A61K36/48A61P35/00A61K31/192
Inventor 付玉杰祖元刚刘霞吴楠孔羽罗猛顾成波彭霄
Owner NORTHEAST FORESTRY UNIVERSITY
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