Method for producing novel lance atractylodes rhizome inoculation agent

Abstract

The invention relates to the field of agricultural seeds, in particular to a method for producing a lance atractylodes rhizome inoculation agent from lance atractylodes rhizome endophytic fungus or probiotic bacteria. The method specifically comprises the following steps: activating the lance atractylodes rhizome endophytic fungus or the probiotic bacteria; adding a small quantity of thickening agents or water retention agents after the lance atractylodes rhizome endophytic fungus or the probiotic bacteria are cultivated on a culture medium containing potato immersion liquid, lance atractylodes rhizome immersion liquid and glucose liquid or a solid medium containing lance atractylodes rhizome straws; and soaking lance atractylodes rhizome seeds and transplanting soaking roots or adding the seeds to rhizoplane soil directly. The inoculation agent is not easily influenced by the environment during the production and has short production period, simple production process, production time, easy control of output and remarkable action. The method adopting the soaking seeds, soaking plant roots or pouring and directly adding the seeds in soil ensures that the inoculation agent acts on plants and has the advantages of simple method, easy operation, less consumption, low cost and good effect.

Description

technical field [0001] The invention belongs to the technical field of agricultural application microorganisms, and in particular relates to a method for producing an inoculant of Atractylodes atractylodes. The bacterial agent produced by the method can overcome or eliminate obstacles to continuous cropping of Atractylodes atractylodes. Background technique [0002] Atractylodes lancea (Thunb.) DC. is a perennial medicinal plant of the genus Atractylodes lancea commonly used in Chinese folk. It was first recorded in "Shen Nong's Materia Medica". It has the effects of drying dampness and invigorating the spleen, expelling wind and cold, and improving eyesight. Atractylodes atractylodis is a famous authentic medicinal material in Jiangsu Province. It has a long history of clinical use in traditional Chinese medicine. Its active ingredients are mainly β-cineol, atractylone, atractylodes alcohol and polyacetylene component atractylodes. Arthralgia, anemofrigid cold and so on. ...

AI Technical Summary

Problems solved by technology

Many places have begun to introduce and cultivate Atractylodes atractylodes, but because the continuous cropping obstacles of Atractylodes atractylodes can not be solved, there are still many problems in the development of Atractylodes atractylodes resources: (1) The germination rate of Atractylodes atractylodes seeds is low, which cannot provide stable seeds for large-scale planting. (2) Artificial cultivation is difficult, and the growth of Atractylodes atractylodes is slow. Both morbidity and mortality have increased. Wang Yixun and others investigated in Xiaochang County and Yingshan County, Hubei Province, an important base of standardized planting of Chinese herbal medicines (GAP), and found that the occurrence of Atractylodes atractylodes black spot is very serious, and the yield of general fields is reduced by about 10% to 15%. Severe field yield loss of up to 90%

However, due to continuous cropping obstacles, low seed germination rate, slow growth, stress resistance, poor resistance to diseases and insect pests, and unstable quality, the cultivation has not been successful, and large-scale production has not been possible, and it has not been solved domestically.

Our experiments have shown that inoculating the endophytic fungi or probiotics of Atractylodes atractylodes will be an effective way, and no one has reported the use of probiotics or endophytic fungi preparations of Atractylodes atractylodes to overcome or eliminate operational obstacles. The production method of this bacterial agent has no patent

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