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Microbial preparation for processing refuse leachate and preparation

A technology for microbial preparations and landfill leachate, which is applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of long start-up time and unsatisfactory effects of biological treatment processes, and achieve enhanced system stability. Strong adaptability and improved removal effect

Inactive Publication Date: 2009-05-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, for water with high salinity (or landfill leachate), when the chloride ion content is as high as 4500-16360 mg / L, the normal biological treatment process takes a long time to start and the effect is not ideal

Method used

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  • Microbial preparation for processing refuse leachate and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation of liquid medium I: Weigh 1 part of magnesium sulfate heptahydrate, 0.2 part of potassium chloride, 2.47 parts of sodium chloride, 0.3 part of sodium citrate, 1 part of yeast extract powder, 0.5 part of tryptone, and add water to 100 parts , adjust the pH to be 7.2 to make liquid medium I;

[0040] Preparation of liquid medium II: weigh 1.2 parts of magnesium sulfate heptahydrate, 0.2 parts of potassium chloride, 2.4 parts of sodium chloride, 0.3 parts of sodium citrate, 0.2 parts of ammonium chloride, 0.5 parts of yeast extract powder, 0.15 parts of pancreatic Peptone, 0.7 part of starch, 0.5 part of glucose, add water to 100 parts, adjust pH to 7.2 to prepare liquid medium II.

Embodiment 2

[0042] Preparation of liquid medium I: Weigh 1 part of magnesium sulfate heptahydrate, 0.2 part of potassium chloride, 2.47 parts of sodium chloride, 0.3 part of sodium citrate, 1 part of yeast extract powder, 0.5 part of tryptone, and add water to 100 parts , adjust the pH to be 7.0 to make liquid medium I;

[0043] Preparation of liquid medium II: weigh 1.2 parts of magnesium sulfate heptahydrate, 0.2 parts of potassium chloride, 2.4 parts of sodium chloride, 0.3 parts of sodium citrate, 0.2 parts of ammonium chloride, 0.5 parts of yeast extract powder, 0.15 parts of pancreatic Peptone, 0.7 part of starch, 0.5 part of glucose, add water to 100 parts, adjust pH to 7.0 to prepare liquid medium II.

Embodiment 3

[0045] Preparation of liquid medium I: Weigh 1 part of magnesium sulfate heptahydrate, 0.2 part of potassium chloride, 2.47 parts of sodium chloride, 0.3 part of sodium citrate, 1 part of yeast extract powder, 0.5 part of tryptone, and add water to 100 parts , adjust the pH to be 7.5 to make liquid medium I;

[0046] Preparation of liquid medium II: weigh 1.2 parts of magnesium sulfate heptahydrate, 0.2 parts of potassium chloride, 2.4 parts of sodium chloride, 0.3 parts of sodium citrate, 0.2 parts of ammonium chloride, 0.5 parts of yeast extract powder, 0.15 parts of pancreatic Peptone, 0.7 part of starch, 0.5 part of glucose, add water to 100 parts, adjust pH to 7.5 to prepare liquid medium II.

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Abstract

The invention discloses a microbial preparation used for treating landfill leachate and a preparation method thereof; the preparation method of the microbial preparation is as follows: (1) a liquid culture medium I and a liquid culture medium II are prepared; (2) bacillus cereus is inoculated into the liquid culture medium I for culture and then inoculated into the liquid culture medium II for mass production, thereby obtaining bacterial suspension; Bacillus marisflavi is inoculated into the liquid culture medium I for culture and then inoculated into the liquid culture medium II for mass production, thereby obtaining the bacterial suspension; photosynthetic bacteria, nitrifying bacteria, denitrifying bacteria and yeast are respectively inoculated into the liquid culture medium II to be cultured into the bacterial suspension after activation; and (3) the microbial preparation is prepared by mixing the various types of bacterial suspension according to proportion. The microbial preparation can improve the removal rate of organic matters which are difficult to decompose and ammonia nitrogen in the salt-containing landfill leachate. The microbial preparation still has very high biological activity when being added into organic waste water with high salt content, thereby improving the removal effect of COD and ammonia nitrogen in the leachate.

Description

technical field [0001] The invention relates to a microbial preparation, a preparation method and an application, in particular to a microbial preparation for treating landfill leachate, a preparation method and an application. Background technique [0002] In recent years, the application of microbial agents has begun to involve the field of environmental protection, especially in the field of water pollution control. Since high-efficiency bacterial agents composed of different microorganisms have different functions, microorganisms with different functions can be selected to form high-efficiency bacterial agents. The use of microbial agents in sewage treatment can increase the removal rate of various pollutants and reduce the sludge yield. Microbial preparations are used as biological enhancers, and the combination of bioaugmentation technology and traditional biological treatment technology is used to treat landfill leachate, which breaks through the inefficiency of cult...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/16C02F3/34C12R1/085C12R1/07C12R1/38C12R1/01C12R1/385C12R1/05C12R1/72C12R1/88C12R1/645
Inventor 季民郭立王芬王景峰
Owner TIANJIN UNIV
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