Clove iridoid glycoside extraction separation technique

A clove iridoid glycoside and process technology, which is applied in the field of clove iridoid glycoside extraction and separation technology, can solve problems that are not found in patent literature, and achieve the effect of reasonable design and simple process

Inactive Publication Date: 2008-11-19
李永吉
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  • Application Information

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Problems solved by technology

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Embodiment 1

[0009] Add 10 times the amount of water to decoct clove leaves, decoct 3 times, 0.5 hours each time, concentrate until the volume of the medicinal liquid and the weight ratio of the original medicinal material are 1:1, add an appropriate amount of 0.5% chitosan, and the alcohol precipitation concentration is 60 %, filter, carry out high-speed centrifugation, put on a macroporous adsorption resin column, collect macroporous adsorption resin 50% ethanol eluate, extract 3 times with ethyl acetate, carry out microporous membrane separation, put on a silica gel column, use chloroform- Gradient elution was performed with methanol at ratios of 30:1, 20:1, 15:1, and 10:1, respectively.

Embodiment 2

[0011] Add 15 times the amount of water to decoct clove leaves, decoct twice, 1 hour each time, concentrate until the volume of the medicinal liquid and the weight ratio of the original medicinal material are 1:2, add an appropriate amount of 1% chitosan, and the alcohol precipitation concentration is 70 %, filtered for high-speed centrifugal separation, put on a macroporous adsorption resin column, collected macroporous adsorption resin 50% ethanol eluate, extracted 4 times with ethyl acetate, carried out microporous membrane separation, put on a silica gel column, and used chloroform-methanol Gradient elution was carried out at the ratio of 30:1, 20:1, 15:1, and 10:1, respectively.

Embodiment 3

[0013] Add 20 times the amount of water to decoct clove leaves, decoct once, 1.5 hours each time, concentrate until the volume of the medicinal liquid and the weight ratio of the original medicinal material are 1:3, add an appropriate amount of 1.5% chitosan, and the alcohol precipitation concentration is 80 %, filtered for high-speed centrifugal separation, put on a macroporous adsorption resin column, collected macroporous adsorption resin 50% ethanol eluate, extracted 5 times with ethyl acetate, carried out microporous membrane separation, put on a silica gel column, and used chloroform-methanol Gradient elution was carried out at the ratio of 30:1, 20:1, 15:1, and 10:1, respectively.

[0014] The extraction and separation process of clove iridoid glycosides in the present invention is applied to modern extraction and concentration tanks. The whole process is airtight without water loss and loss of medicinal liquid components. The concentrated liquid is precipitated with eth...

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Abstract

The invention relates to a process for extracting and separating lilac iridoid glycoside. Lilac leaves are aqueously extracted, condensed by depressurization, defecated in chitosan, deposited in ethanol, filtered, centrifugally separated, absorbed by macroporous adsorbing resin, and eluted with ethanol; ethanol is recycled from eluent; the filter liquor is extracted by ethyl acetate, and is separated through a millipore filter before the filter liquor is extracted and separated with silicagel columns; gradient elution is conducted with chloroform and methanol; solvent is recycled from the eluent. The thin-layer chromatography is used for detecting lilac iridoid glycoside, and the high efficiency liquid chromatography is used for measuring the lilac iridoid glycoside with the purity larger than 90 percent. The process provided by the invention is simple and designed reasonably. The product obtained through the process can meet medicinal requirement. The lilac iridoid glycoside has the functions of antibiosis, diminishing inflammation and antivirus, and can inhibit golden yellow Staphylococcus, Bacillus dysenteriae, colibacillus, Pseudomonas aeruginosa and so on. The process is used for curing such diseases as upper respiratory tract infection, bacillary dysentery and so on.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extraction and separation process, in particular to a clove iridoid glycoside extraction and separation process. Background technique [0002] Traditional Chinese medicine extraction methods are mainly leaching method, percolation method, modified gelatin method, reflux method and so on. Among them, boiling in water is the most commonly used method. There are many shortcomings in the traditional method. In order to improve the quality of traditional Chinese medicine, change the state of traditional dosage forms of "big, dark, and thick" and let Chinese medicine enter the international market, some modern high-paying engineering technologies are being continuously used for reference in the production of Chinese medicine. On the one hand, the existing Chinese medicine resources are improved. Utilization, on the other hand, also makes the production of traditional Chinese medicine more in line with...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P31/04
CPCY02A50/30
Inventor 李永吉
Owner 李永吉
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