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Preparation technology of promoting hepatocyte growth factor solution

A hepatocyte auxin and preparation process technology, applied in the field of hepatocyte auxin solution preparation process, can solve the problems of long production cycle, troublesome operation, low yield, etc., and achieve low production cost, simplified operation, and high production yield high effect

Inactive Publication Date: 2008-07-23
HANGZHOU HUAJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the repeated freezing and thawing method needs to be carried out 4 to 5 times, which has the disadvantages of troublesome operation, long production cycle, low yield and high cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation process of the hepatocyte growth-promoting hormone solution includes material processing, active ingredient extraction, heat denaturation, solid-liquid separation and ultrafiltration, and pH value adjustment. The material processing mainly includes liver cleaning and liver tissue crushing. The extraction of active ingredients in this example adopts the extraction method of chemical active ingredients by enzymatic hydrolysis, and the specific preparation process includes the following six steps.

[0021] 1. Material liver cleaning steps: remove the fat and fascia of fresh suckling pig liver or small fetal bovine liver, and wash the blood in the liver with water.

[0022] 2. Liver tissue crushing step: chop the cleaned liver, add clean water, and carry out liver tissue crushing. In this embodiment, the liver tissue is crushed by colloid mill homogenization, and other crushing methods such as ultrasonic wave can also be used.

[0023] 3. Active ingredient ...

Embodiment 2

[0028] The present embodiment adopts the acid hydrolysis chemical active ingredient extraction method, and the operation of the active ingredient extraction step of the acid hydrolysis extraction method is as follows:

[0029] Add water of 1 times the weight of the liver pulp to the crushed liver pulp after "a, material liver cleaning step" and "b, liver tissue crushing step", stir evenly to make a homogenate, and adjust the pH value of the homogenate to After 1.0 to 6.0, put it into a freezer at a temperature of -5 to -30°C to freeze for 5 to 40 hours, take it out after freezing and thaw it in a water bath below 45°C, and then adjust the thawed acid hydrolyzate with sodium hydroxide pH value to 6.0 ~ 7.0. The acid used in the acid hydrolysis extraction method in this embodiment is hydrochloric acid, phosphoric acid or acetic acid, the temperature of the cold storage is controlled at -10±2°C, and the freezing time is 15±5 hours.

[0030] Other operating steps of this embodime...

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Abstract

The invention discloses a preparation technique for promoting hepatocyte growth factor solution, which comprises a hepatic-cleaning step, a hepatic tissue smashing step, an active component extracting step, a heating and denaturing step, a solid-liquid separation and pH value regulating step. The preparation technique is characterized in that: the active components extracting is a chemical extraction method of enzymatic hydrolysis or acid hydrolysis; enzyme for the enzymatic hydrolysis extraction method is about papain or tryptic enzyme or stomach enzyme or neutral protease; acid of the acid hydrolysis extraction method is about hydrochloric acid or phosphoric acid or acetic acid. The preparation technique for promoting hepatocyte growth factor solution has the advantages of overcoming the drawback of repeated freeze thaw method for prior art, simplifying the preparation operation for promoting hepatocyte growth factor solution, shortening production period, fully being in conformity with relative standard requirement upon national drug regulated by State Drug Administration for prepared promoting hepatocyte growth factor solution, having high preparation rate and low production cost.

Description

technical field [0001] The invention relates to a preparation process of biochemical medicines, in particular to a preparation process of hepatocyte growth-stimulating factor solution. Background technique [0002] Hepatocyte growth-promoting factor pHGF is a biochemical drug, which is mainly used to treat various severe hepatitis, chronic active hepatitis and liver cirrhosis. The main raw material for the preparation of the hepatocyte growth-promoting factor pHGF is a hepatocyte-promoting factor solution, and the hepatocyte-promoting factor solution is a biologically active polypeptide solution extracted from fresh suckling pig liver or calf liver. In the prior art, the preparation process of the hepatocyte growth-stimulating hormone solution is divided into two steps, one is material treatment, and the other is the preparation of the hepatocyte growth-stimulating hormone solution. The key process for the preparation of hepatocyte growth-promoting hormone solution. The act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/12C07K1/34C12P21/06A61K38/17A61P1/16
Inventor 周正兵高留根俞保彬
Owner HANGZHOU HUAJIN PHARMA
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