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Dual encapsulating material of retinene membrane protein and method of producing the same

A technology of retinaldehyde membrane protein and encapsulation material, which is applied in the field of double encapsulation material of retinaldehyde membrane protein with optical response and its preparation, can solve the problems of no material specification, influence on technical exchange and industrial production, and time-consuming

Inactive Publication Date: 2008-02-27
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that on the one hand, due to the dependence of the material on the environment, it needs to be tested and stored in a short period of time, or it takes time and energy to adjust the environmental conditions to protect the material during testing or storage; on the other hand, there is no standard for the material. Specifications make it non-versatile and universal in application, affecting technical exchanges and industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Liquid paraffin encapsulation of bacteriorhodopsin-polyvinyl alcohol composite film

[0021] Weigh 3.3g of polyvinyl alcohol (PVA) with a molecular weight of 80,000 and a degree of alcoholysis of 98%, add it to 30mL of water, heat it to 90°C and stir until completely dissolved to obtain a 10% (wt) transparent solution, which is cooled and set aside. Will be from R 1 m 1 The bacteriorhodopsin protein suspension extracted from the strain was added to 1 mL of polyvinyl alcohol solution, and the protein concentration was 10 mg / mL. The mixed solution was ultrasonically dispersed on a YJ92-II ultrasonic cell pulverizer, with a power of 150w, ultrasonic time of 4s, interval of 40s, and ultrasonication 10 times to obtain a clear and transparent mixed solution. Absorb an appropriate amount of liquid and spread it on a square film with a side length of 1cm and a depth of 5mm. hole, put in a desiccator, and dry at 4°C for 24 hours to obtain a bacteriorhodopsin-polyvinyl alcohol ...

Embodiment 2

[0024] Silane Encapsulation of Archaeordopsin 4-Gelatin Composite Films

[0025] Weigh 1g of gelatin particles, add it to 10mL water, stir in a hot water bath at 60°C until it dissolves completely, and obtain a gelatin solution with a concentration of 10% (wt), freeze-dry the archerhodopsin 4 protein extracted from bacterial strain xz515 into a dry powder, weigh Suspend 40 mg of dry protein powder in 0.5 mL of water, ultrasonically disperse it on a YJ92-II type ultrasonic cell pulverizer, with a power of 100 W, ultrasonic time of 5 s, interval of 30 s, ultrasonic 15 times, absorb 0.5 mL of 10% gelatin solution, and add it to the protein suspension, Stir and mix in a hot water bath, ultrasonically disperse again, power 100w, ultrasonic time 5s, interval 30s, ultrasonic 15 times to obtain a clear and transparent mixed solution, filter the mixed solution with a 5μm. Put in a circular membrane hole with a diameter of 1.5cm and a depth of 1mm, place it at 4°C until it becomes a gel...

Embodiment 3

[0028] n-Hexane Encapsulation of Bacteriorhodopsin D96N Mutant-Polyvinyl Alcohol Composite Film

[0029] Weigh 3.3g of polyvinyl alcohol (PVA) with a molecular weight of 80,000 and a degree of alcoholysis of 98%, add it to 30mL of water, heat it to 90°C and stir until completely dissolved to obtain a 10% (wt) transparent solution, which is cooled and set aside. The freeze-dried powder of bacteriorhodopsin D96N protein was added to 1 mL of polyvinyl alcohol solution, and the protein concentration was 20 mg / mL. The mixed solution was ultrasonically dispersed on a YJ92-II ultrasonic cell pulverizer, with a power of 180w, ultrasonic time of 3s, and an interval of 40s, and ultrasonication 5 times to obtain a clear and transparent mixed solution, absorb an appropriate amount of liquid, and spread it in a hexagon with a side length of 0.5cm and a depth of 5mm Put the film into the hole of the film tool, put it into a desiccator, and dry it at 4°C for 24 hours to obtain a bacteriorhod...

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PUM

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Abstract

The invention discloses a dual packing material of retinyl membrane protein with optical response and making method in the macromolecular material and information material technical domain, which is characterized by the following: adopting once package of protein dispersing and fixing as goal to connect twice package based on separation and protection, appearance control and surface modification of optical property as goal; making the packed material as optical element keep good property in the storing, assembling, detaching and transporting course; fitting for preparing standardized element; increasing the fitting scale; providing beneficial condition for technical communication and scaling usage.

Description

technical field [0001] The invention belongs to the technical field of polymer materials and information materials, and specifically relates to a double-encapsulation material of retinal membrane protein with optical response and a preparation method thereof. Background technique [0002] Retinaldehyde membrane proteins that respond to light activity include bacterial rhodopsin (bacteriorhodopsin, BR), archaerhodopsin (Archaerhodopsin, such as AR4, see Li, Q.G. et al, BBA 1466, 260-266, 2000; Ming, M. Biophys.J.90, 3322-3332, 2006) and their mutants mixed with polymers have broad application prospects in the field of optical storage and processing, and can be applied to optical information storage (including two-dimensional storage , three-dimensional storage and holographic storage, etc.) and optical information processing (including light conversion, light filtering and signal conditioning, etc.). (Hampp, N. Chem. Rev. 2000, 100, 1755-1776). [0003] These composite mate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K3/10C08L29/04C08L89/00C08L83/14C08K5/17C08J5/18C07K14/195
Inventor 丁建东吴佳马德旺
Owner FUDAN UNIV
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