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Coenzyme composition preparing method

A compound and coenzyme technology, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, can solve the problems of reduced ultrafiltration effects, incoherent connections, and high requirements for operators to achieve lower requirements, The effect of reducing the process links and reducing the difficulty of separation

Inactive Publication Date: 2007-08-29
NANTONG XINPING GARMENTS
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

The disadvantages are: 1. There is no coherent connection between the various processes, and intermediate processing is required before entering the next process
3. Put ultrafiltration after ion exchange resin purification, so that the effect of ultrafiltration is greatly reduced
4. The process links are cumbersome and have high requirements for operators

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Mix the baker's yeast with water and ice at a ratio of 1:1:2, place in 1 volume of boiling water, keep warm at 90°C for 5 minutes, and quickly put in crushed ice to cool. Centrifuge at 4800rpm for 30min, and take the supernatant.

[0015] 2. The supernatant is placed in an ultrafilter equipped with a cut 10000 molecular weight ultrafiltration membrane, separated and purified under 0.2MPa ultrafiltration pressure, and the filtrate is taken.

[0016] 3. Purify the filtrate by column chromatography with 717 strong base anion exchange resin, elute with 0.2mol / L NaCl (adjust the pH to 1.7 with 0.1mol / L HCl), and use a peristaltic pump to control the flow rate during elution At 200mL / h, the nucleic acid protein detector monitors the OD value of the effluent in real time, and collects the part that absorbs light at a wavelength of 254nm.

[0017] 4. Put the eluate in a rotary evaporator and concentrate under reduced pressure at 58°C to 1 / 20 of the original volume.

[0018...

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PUM

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Abstract

The present invention relates to a preparation method of coenzyme compound. Said invention adopts mature technical processes of temperature difference wall breakage and reduced pressure concentration, and utilizes the steps of ultrafiltering separation, column chromatography to make purification, concentration and precipitation so as to obtain the invented coenzyme compound.

Description

technical field [0001] The invention relates to a preparation method of a coenzyme complex. Background technique [0002] The coenzyme complex is composed of a variety of coenzymes and biologically active substances, mainly containing biologically active substances such as coenzyme A, coenzyme I, adenosine triphosphate, reduced glutathione and nucleotides. Most of them are coenzymes of important enzymes in acetylation reaction, redox reaction, transmethylation reaction and energy metabolism in the human body. They play an important role in the metabolism of sugar, protein, fat and energy in the body. , Fatty acid β-oxidation, synthesis and decomposition of liver glycogen, synthesis of acetylcholine, tissue respiration, energy transfer, liver protection and detoxification, anti-radiation (radiation) effects and other aspects are closely related to it, and its product market prospect is very broad. [0003] At present, domestic production of compound coenzymes is mainly based...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61P3/00A61P43/00
Inventor 沈忠明朱俊毅殷建伟
Owner NANTONG XINPING GARMENTS
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