Transformant containing transaldolase gene
a transaldolase and gene technology, applied in the direction of transferases, enzymology, microorganisms, etc., to achieve the effect of increasing the ability to produce the intended aromatic compounds, increasing the transaldolase activity, and increasing the ability to produce the substances
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. THE INVENTION
(1) Acquisition of a Transketolase-Deficient Mutant of Corynebacterium Glutamicum:
[0128]Corynebacterium glutamicum L22 [a lysozyme-sensitive mutant derived from a wild type strain ATCC 31833; R. Katsumata et al., Proc. 4th Eur. Congr. Biotechnol., 4, 767 (1987)] was inoculated in 3 ml of NB medium [containing 20 g of bouillon powder and 5 g of yeast extract in water 1 liter and having pH 7.2] and cultured therein at 30° C. until OD660 of the culture reached to 0.6.
[0129] After culturing, the cells were recovered through centrifugation, and washed once with 50 mmol / l Tris maleate buffer (pH 6.0), and subjected to a mutational treatment in 3 ml of the buffer containing 400 mg / l of NTG, at room temperature for 20 minutes. The treated cells were centrifuged and washed twice with the buffer, and then cultured in 3 ml of NB medium at 30° C. for 1 hour.
[0130] The culture was diluted with physiological saline to 10−5 to 10−6, and 0.1 ml of the resulting dilution was sprea...
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