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Transformant containing transaldolase gene

a transaldolase and gene technology, applied in the direction of transferases, enzymology, microorganisms, etc., to achieve the effect of increasing the ability to produce the intended aromatic compounds, increasing the transaldolase activity, and increasing the ability to produce the substances

Inactive Publication Date: 2005-12-01
KYOWA HAKKO KOGYO CO LTD
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AI Technical Summary

Benefits of technology

The present invention relates to a new transaldolase gene and the polypeptide it encodes. The invention also includes a process for producing the polypeptide using a recombinant DNA technique. The polypeptide has been found to have activity in the production of aromatic amino acids, aromatic vitamins, L-histidine, riboflavin, nucleic acids, nucleic acid-associated substances, and saccharides. The invention provides a novel way to produce these compounds using a recombinant DNA technique.

Problems solved by technology

However, for microorganisms belonging to the genus Corynebacterium that are widely used in amino acid fermentation of industrial importance, there is no report relating to the transaldolase gene and the enzyme encoded by the gene, and the nucleotide sequence of that gene is not known at all.

Method used

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Examples

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Embodiment Construction

. THE INVENTION

(1) Acquisition of a Transketolase-Deficient Mutant of Corynebacterium Glutamicum:

[0128]Corynebacterium glutamicum L22 [a lysozyme-sensitive mutant derived from a wild type strain ATCC 31833; R. Katsumata et al., Proc. 4th Eur. Congr. Biotechnol., 4, 767 (1987)] was inoculated in 3 ml of NB medium [containing 20 g of bouillon powder and 5 g of yeast extract in water 1 liter and having pH 7.2] and cultured therein at 30° C. until OD660 of the culture reached to 0.6.

[0129] After culturing, the cells were recovered through centrifugation, and washed once with 50 mmol / l Tris maleate buffer (pH 6.0), and subjected to a mutational treatment in 3 ml of the buffer containing 400 mg / l of NTG, at room temperature for 20 minutes. The treated cells were centrifuged and washed twice with the buffer, and then cultured in 3 ml of NB medium at 30° C. for 1 hour.

[0130] The culture was diluted with physiological saline to 10−5 to 10−6, and 0.1 ml of the resulting dilution was sprea...

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Abstract

The present invention relates to a novel transaldolase gene, and to a polypeptide encoded by the gene, a recombinant DNA obtained by ligating the gene, a transformant carrying the recombinant DNA, and a process for producing the polypeptide, aromatic amino acids, aromatic vitamins, L-histidine, riboflavin, nucleic acids, nucleic acid-associated substances, novel saccharides and others by utilizing the transformant.

Description

TECHNICAL FIELD [0001] The present invention relates to a novel transaldolase gene, and to a polypeptide encoded by the gene, a recombinant DNA obtained by ligating the gene, a transformant carrying the recombinant DNA, and a process for producing the polypeptide, aromatic amino acids, aromatic vitamins, L-histidine, riboflavin, nucleic acids, nucleic acid-associated substances, novel saccharides and others by utilizing the transformant. BACKGROUND ART [0002] Transaldolase is an enzyme involved in pentose phosphate pathways, and plays an important role in biosynthesis and metabolism of aromatic compounds such as aromatic amino acids and aromatic vitamins, nucleic acid-associated substances such as purine-nucleotide and pyrimidine-nucleotide, as well as L-histidine, riboflavin and others [Arch. Microbiol., 164, 324 (1995)]. Accordingly, a transaldolase gene and its gene products are useful as the target in breeding microorganisms for efficient fermentation and production of the metab...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N1/21C12N9/10C12N15/54
CPCC12Y202/01002C12N9/1022C12N9/10
Inventor IKEDA, MASATOTAKANO, YUTAKANAKANO, TETSUOKAMADA, NOZOMU
Owner KYOWA HAKKO KOGYO CO LTD
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