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Inspection of manyprickle acanthopanax

A detection method, the technology of Acanthopanax senticosus, applied in the direction of measuring devices, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problem of low sensitivity and achieve the effect of good selectivity and low detection limit

Inactive Publication Date: 2007-01-17
CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

UV detection has good versatility, but the short capillary light path makes it less sensitive

Method used

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  • Inspection of manyprickle acanthopanax
  • Inspection of manyprickle acanthopanax

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Quantitative detection and fingerprint identification analysis of Acanthopanax stems from different origins

[0026] (1) The preparation of buffer solution, its concrete method is:

[0027] Direct mixing of NaH 2 PO 4 and Na 2 B 4 o 7 0.20mol L -1 HCl or 0.20mol L -1 NaOH adjustment.

[0028] (2) Preparation of active ingredient standard solution and cyclic voltammetry experiment thereof, the process is as follows:

[0029] Preparation of standard solution: the concentration is 5.0×10 -3 mol L -1 The solutions of isoferidin, rutin and chlorogenic acid were dissolved in methanol respectively, stored in a refrigerator at 4°C, and diluted to the desired concentration with secondary water before use.

[0030] Cyclic voltammetry experiment of the standard solution: first place a 33 μm CFE electrode in NaH 2 PO 4 and Na 2 B 4 o 7 The mixed solution was scanned for one week as a background. Then this electrode is respectively contained in 1.0×10 -4 ...

Embodiment 2

[0045] Example 2: Quantitative detection and fingerprint identification analysis of different parts of the herbal medicine Acanthopanax

[0046] (1) The preparation of buffer solution, its concrete method is:

[0047] Direct mixing of NaH 2 PO 4 and Na 2 B 4 o 7 0.20mol L -1 HCl or 0.20mol L -1 NaOH adjustment.

[0048] (2) Preparation of standard solution and cyclic voltammetry experiment thereof, the process is as follows:

[0049] Preparation of standard solution: the concentration is 5.0×10 -3 mol L -1 The solutions of isoferidin, rutin and chlorogenic acid were dissolved in methanol respectively, stored in a refrigerator at 4°C, and diluted to the desired concentration with secondary water before use.

[0050] Cyclic voltammetry experiment of the standard solution: first place a 33 μm CFE electrode in NaH 2 PO 4 and Na 2 B 4 o 7 The mixed solution was scanned for one week as a background. Then this electrode is respectively contained in 1.0×10 -4 mol L -...

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Abstract

A method for testing acanthopanax bark features that the simple ultrasonic extracting in methanol is combined with the capillary electrophoresis type electrochemical analysis method, and the quality of acanthopanax bark is determined by comparing the contents of active components and the fingerprint.

Description

technical field [0001] The invention belongs to a detection method of traditional Chinese medicine Acanthopanax, in particular to a capillary electrophoresis electrochemical detection method of Acanthopanax. Background technique [0002] Acanthopanax is a herb widely grown in Hebei, Jilin, Liaoning and North China. But only Acanthopanax produced in Heilongjiang Province is considered authentic. In the Chinese Pharmacopoeia, the Chinese herbal medicine Acanthopanax is the dried root, rhizome or stem of the Araliaceae plant Acanthopanax senticosus (Rupr. Et Maxim.) Harms. In addition, the leaves of the plant Acanthopanax can also be used medicinally. The medicinal activity of Acanthopanax is similar to that of ginseng, and it has the effect of strengthening the body. Therefore, the study on eleuthero has aroused extensive interest of researchers. Isoferidin, chlorogenic acid and rutin are the important bioactive components in Acanthopanax. Among them, the content of isofe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/254G01N27/447
Inventor 由天艳周晓光
Owner CHANGZHOU INST OF ENERGY STORAGE MATERIALS &DEVICES
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