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Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine

A technology of water extract and determination method, which is applied in the field of content determination of aristolochic acid A in water extract of traditional Chinese medicine, and can solve the problems of low content and incapable of quantitative detection, etc.

Inactive Publication Date: 2006-10-18
TIANJIN TASLY PHARMA CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

Because aristolochic acid A is insoluble in water, even if there is co-dissolution and solubilizing effect, the content of aristolochic acid A in aqueous solution is still very low, even if the high performance liquid chromatography method with high sensitivity is adopted, Can not be quantitatively detected

Method used

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  • Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine
  • Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine
  • Method of detecting content of micro amount of aristolochic acid A in water extract of Chinese medicine

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Effect test

Embodiment 1

[0015] Example 1 and Comparative Example: Determination of Aristolochic Acid A Content in Asarum Water Extract

[0016] Asarum is a traditional Chinese medicine containing aristolochic acid A. In industrial production, water is often used as the extraction solvent. Pure aristolochic acid A is insoluble in water, but, due to the aiding and co-dissolution of other components in Asarum to Aristolochic Acid A, Aristolochic Acid A in Asarum can be extracted in a small amount, Since aristolochic acid A is a toxic component, it is necessary to accurately determine the content of aristolochic acid A in the water extract. The method of the invention can accurately measure the content of aristolochic acid A in the water extract.

[0017] 1. Instruments, medicines and reagents

[0018] Agilent 1100 high performance liquid chromatography equipped with a quaternary pump, an online vacuum degasser, a diode array detector, and a column oven. Asarum (provided by Tasly Modern Chinese Medic...

Embodiment 2

[0030] Embodiment two: Aristolochic acid A content determination in Guan Mutong water extract

[0031] Preparation of the test solution: get 6g of Akebia officinalis medicinal material powder, accurately weigh, put in a 250mL flask, add 200mL of water, heat and extract for 30min, centrifuge, get the supernatant 100mL, put it on a well-processed AB-8 small column (weigh 200mL ~400 mesh AB-8 packing 4g, placed in a glass column with an inner diameter of 2cm, rinsed with 40mL of methanol, and then washed with 40mL of water, and set aside; the macroporous resin packing AB-8 was purchased from Nankai University Chemical Factory), and the sample loading and washing were controlled. The flow rate of the removal process is not greater than 0.7mL·min-1, rinse with 10mL of 30% methanol, and then rinse with 10mL of acetone, collect the acetone solution, dilute to 10mL, shake well, and obtain.

[0032] Others are the same as in Embodiment 1

[0033] Results: The content of aristolochic a...

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Abstract

The method of detecting micro content of aristolochic acid A in water extract of Chinese medicine includes extracting the Chinese medicine sample with water to obtain water extract of Chinese medicine, treating the water extract by means of solid extracting technology to obtain sample solution, and high efficiency liquid chromatography detecting, with the stuffing for solid extracting being macroporous resin stuffing. The present invention has accurate determination of micro content of aristolochic acid A in water solution.

Description

technical field [0001] The invention relates to a method for determining the content of toxic components in the water extract of traditional Chinese medicine, in particular to a method for determining the content of aristolochic acid A in the water extract of traditional Chinese medicine. Background technique [0002] Aristolochic acid A is a toxic ingredient contained in traditional Chinese medicine, which can cause kidney damage. The traditional Chinese medicines containing aristolochic acid A are mainly Aristolochiaceae plants, mainly including Aristolochia aristolochia, Guan Mutong, Guangfangji, Qingmuxiang, Tianxianteng, cinnabar lotus, Xugufeng, and Asarum. Xin, Du Heng, Akebia, Akebia, Clematis, Akebia, Clematis, Phyllostachys chinensis, Stephania, Fangji, Akira, Chuanxiang, Bat Pueraria The plant northern bean root, the genus of the genus Woody. Pure aristolochic acid A has good solubility in organic solvents such as methanol, but it is difficult to dissolve in wat...

Claims

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Application Information

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IPC IPC(8): G01N30/02B01D11/00G01N30/06G01N30/38G01N30/60
Inventor 高钧魏峰
Owner TIANJIN TASLY PHARMA CO LTD
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