Sea island cotton pollen tube passage method transgene technology

A pollen tube passage and transgenic technology, applied in the field of plant genetic engineering and agricultural biology, can solve the problems of difficult establishment of somatic cell regeneration system, prone to genotype variation, high transformation cost, etc., and achieve improved sea-island cotton varieties and high bell seat rate , The effect of high breeding efficiency

Active Publication Date: 2006-05-10
SINKIANG YIDA AGRI TECH
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Problems solved by technology

The main disadvantages of the latter two methods are: a good somatic cell regeneration system must be established, but cotton is a relatively difficult crop for plant somatic tissue culture, embryogenesis has obvious genotype restrictions, and the somatic cell regeneration system is limited in general varieties. Difficult to establish; and prone to genotypic variation during somatic tissue culture; relatively high transformation costs
Therefore, using the existing pollen tube channel transgenic technology, as described in documents CN1229141A and CN1251862A, its transformation rate is far lower than that of upland cotton, almost zero

Method used

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  • Sea island cotton pollen tube passage method transgene technology
  • Sea island cotton pollen tube passage method transgene technology
  • Sea island cotton pollen tube passage method transgene technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Transfer protein gene (GhABC1) into sea island cotton variety Xinhai No. 16 to cultivate a new sea island cotton variety with improved fiber quality

[0037] Xinhai No. 16 (provided by the Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences) was planted to flowering, according to the "Guidelines for Molecular Cloning Experiments" (translated by Jin Dongyan, Li Mengfeng, etc., J. Sambrook, E.F. Fritsch, T. Maniartis) Science Press, 1992) Extraction of plasmid DNA containing transgene. Specifically, it is performed as follows.

[0038] The Escherichia coli strain containing the transgene gene was provided by the Institute of Plant Physiology and Biochemistry, Chinese Academy of Sciences. The strain was inoculated into 30ml of LB liquid medium (Luria-Bertani) containing the corresponding antibiotics, and each liter of medium was prepared in 950ml of distilled water. Add: 10 g tryptone, 5 g yeast extract, 10 g NaCl, shake the container until t...

Embodiment 2

[0071] Example 2. The Expansin gene (Expansin gene) was transferred into the sea-island cotton variety Xinhai No. 17, and the new sea-island cotton variety of improved fiber quality was cultivated

[0072] Xinhai No. 17 (provided by Alar Agricultural Science Institute, First Agricultural Division, Xinjiang) was planted until flowering, and the plasmid DNA containing the extensin gene was extracted according to the "Molecular Cloning Experiment Guide". details as follows.

[0073] Escherichia coli containing the extensin gene was provided by the Institute of Microbiology, Chinese Academy of Sciences, the strain was inoculated into 30ml of LB liquid medium (Luria-Bertani) containing the corresponding antibiotics, and each liter of medium was prepared, and should be added in 950ml of distilled water: pancreatic 10 g peptone, 5 g yeast extract, 10 g NaCl, shake the container until the solute is completely dissolved, adjust the pH to 7.0 with 5M NaOH, add distilled water to a total...

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Abstract

This invention relates to an island cotton improving technique, which maily relates to an island cotton pollen channel transfer method, this method is simple and effective, which changes the lower transfrom rate of the present pollen channel technique on inland cotton. Besids, this invention also gets island cotton with transformed outer gene.

Description

technical field [0001] The application relates to a sea-island cotton quality improvement technology, which belongs to the fields of plant genetic engineering and agricultural biotechnology. More specifically, the present application relates to a sea-island cotton powder tube channel transgenic method, and the sea-island cotton obtained by transferring an exogenous target gene by this method. Background technique [0002] Cotton belongs to Dicotyledoneae, Malvaceae, Gossypium L. in classification, and is one of the most important cultivated crops in the world. Native to high temperature, drought, short-day tropical and subtropical desert grasslands, perennial. After long-term cultivation and domestication by humans, it has gradually become an annual cultivated crop. Cotton is the only crop that produces fiber from its seeds. Cotton fiber is the main raw material of the textile industry; cottonseed contains oil and protein, which is the raw material of the food industry; c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N15/87C12N15/89
Inventor 张玉高万旭中刘霞杨克锐黄全生王冬梅黄乐平李建平
Owner SINKIANG YIDA AGRI TECH
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