Chinese caterpillar fungus bionic culturing method
A Cordyceps sinensis fungus and culture method technology, applied in the field of microbial culture in the biological field, can solve the problems of large-scale production that is difficult to industrialize, gene cloning is impossible to talk about, and commercial production cannot be achieved, so as to achieve sufficient oxygen and reliable pure culture , Conducive to the effect of large-scale production
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Embodiment 1
[0036] Inoculate the aseptic Cordyceps sinensis mycelium in soybean powder 6g / L, peptone 4g / L, glucose 100g / L, yeast extract 0.4g / L, K 2 HPO 4 0.8g / L, MgSO 4 0.4g / L, pH 6 sterile liquid culture medium, placed statically on a culture rack, the culture temperature was 18°C, the pressure was normal pressure, and the light intensity was indoor natural light.
[0037] The mycelium was harvested after six months.
Embodiment 2
[0039] The culture procedures were all carried out as in Example 1, except that the culture container was placed on a magnetic stirrer and stirred for 0.5 hours (18° C.) every month during the culture period to break the mycelia.
Embodiment 3
[0041] 1. Isolation of Trichosporum sinensis from Cordyceps sinensis
[0042] (1) Collection of Cordyceps strains
[0043] In July 2002, in Kangding, Sichuan Province, with the help of local drug farmers, a number of Cordyceps sinensis complete fungus complexes were excavated from Yajiageng with strong and plump fruiting bodies and obvious fertile parts, and put them in an incubator at 15°C for maintenance. Humidity, the soil should not agglomerate after light kneading by hand.
[0044] (2) Ascospore collection and germination
[0045] Remove impurities on the surface of the fruiting body, rinse with sterile distilled water, disinfect the surface with 75% alcohol, place it in an incubator at 15°C, and record and observe every day. When the ascus starts to eject the ascospores, put the fruiting bodies that start to eject the spores into a penicillin vial filled with sterilized physiological saline, fully elute the ascospores, and place the eluted ascospores in an incubator at...
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