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Aspergillus oryzae fungus and its application in production of Pu'er tea

A technology of Aspergillus oryzae and Pu'er tea, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., to achieve the effects of shortening processing time, improving quality, stabilizing quality and improving

Inactive Publication Date: 2006-03-29
秘鸣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to improve the quality of Pu’er tea through the research of microorganisms in Yunnan Pu’er tea processing, and screening beneficial and dominant microbial strains from Pu’er tea processing for production has not yet been published in the literature.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: the purification of PAsp0502 strain

[0013] From the collected tea samples, the aspergillus oryzae strains are obtained by known plate separation. Aspergillus oryzae purified medium: 200g potato, 20g glucose, 20g agar, 1000ml distilled water, natural pH. Peel the potatoes, cut them into pieces, cook them for half an hour, then filter them with gauze, add agar and sugar, melt them and add water to 1000ml. Sterilize at 121°C for 20 minutes.

[0014] Purification method:

[0015] (1) Melt the solid culture medium, cool it to 45°C, pour it into a sterile petri dish, about 15-20 ml per dish, shake it slightly, let it stand still, and make it into a plate for later use.

[0016] (2) Take 2g of the tea sample to be separated, add it to a mortar with 98ml of sterile water beside the flame and grind it, and what is obtained at this time is 10 -2 bacterial suspension.

[0017] (3) Dilution: Dilute the bacterial suspension to 10 -8 The ten-fold method means th...

Embodiment 2

[0021] Embodiment 2: the production application of PAsp0502

[0022] The raw tea is wet-heated according to the traditional method, and then the raw tea is inoculated with 0.1-0.8% PAsp0502 by weight to allow it to ferment. When the pile temperature rises to about 37°C, PAsp0502 starts to metabolize, and further increases with the pile temperature. Elevated, secreting a large amount of glucoamylase, glucoamylase, protease, cellulase and pectinase. During the large-scale production of Pu'er tea, the bacteria secrete enzymes to act on the substrate. Under the action of amylase, the starch in the raw material is degraded into dextrin and various low-molecular sugars, such as maltose, glucose, etc.; under the action of protease, the non-digestible macromolecular protein is degraded into peptone, polypeptide and Various amino acids; under the action of pectinase, glucoamylase, and cellulase, the macromolecular insoluble substances are converted into water-soluble small molecular s...

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PUM

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Abstract

An Aspergillus oryzae Pasp 0502 (CGMCC No.1374) is prepared through culturing in natural culture medium and purifying culture. It can be used in the production of Pu'er tea for increasing the contents of theapolyose and bisterin in Pu'er tea and shortening the productive period.

Description

Technical field: [0001] The invention relates to an aspergillus oryzae fungus and its application in the production of Pu'er tea, belonging to the field of biological technology. Background technique: [0002] Aspergillus is well known. There are a variety of powerful enzyme systems that can be used in industrial production, such as amylase used in starch saccharification, liquefaction and alcohol industry or the manufacture of glucose and digestive agents. Pectinase is used to hydrolyze polygalacturonic acid, and the cellulase produced is C x Enzyme, containing strong protease and pectinase, it can still function when the pH value of the substrate reaches 3.0. Aspergillus oryzae can decompose organic matter to produce various organic acids such as ascorbic acid, citric acid, gluconic acid and gallic acid. This strain can also perform hydroxylation at the 11-α position of steroids. [0003] During the large-scale production of Pu'er tea, the strain can secrete enzymes to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23F3/10C12R1/69
Inventor 周红杰龚加顺秘鸣赵龙飞
Owner 秘鸣
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