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Analeptic rapid testing biological chip and testing method

A detection method and biochip technology, applied in biological testing, material inspection products, fluorescence/phosphorescence, etc., can solve problems such as long detection time

Inactive Publication Date: 2004-12-08
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In the prior art, the determination of these substances is generally in accordance with the traditional prescribed determination method, and the detection time is relatively long

Method used

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  • Analeptic rapid testing biological chip and testing method
  • Analeptic rapid testing biological chip and testing method
  • Analeptic rapid testing biological chip and testing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Detection of stimulants related to adrenergic β receptors

[0029]Agonists (stimulants) associated with beta-adrenoceptors mainly include partial beta-receptor agonists used as stimulants such as bambuterol, clenbuterol, fenoterol (fenoterol), salbutamol (salbutamol), tea proterol (reproterol), terbutaline (terbutaline) and zamoterol (xamoterol), etc., as stimulants, these drugs can enhance human neurological function by acting on the nervous system. Mental and physical strength, its serious side effects are to cover up fatigue and lead to excessive excitement and anxiety, affecting the judgment of athletes, often causing injuries, and causing a rapid increase in heart rate and blood pressure. In addition, it may cause dehydration, induce cerebral hemorrhage and heart disease.

[0030] The other is adrenergic β-receptor blockers, mainly including acebutolol, alprenolol, propranolol, oxprenolol, atenolol, bisso Bisoprolol, labetolol, metoprolol, etc. These dru...

Embodiment 2

[0047] Example 2 Detection of steroid agonists related to androgen receptor.

[0048] Anabolic steroids, also called anabolic hormones. The most commonly used ones are: metandienone, oxymetholone, mesterolone, testosterone, etc. These drugs can be said to be the class with the highest frequency and widest range as stimulants. These drugs, taken orally or injected, bind to androgen receptors in the body to produce androgen-like effects that can strengthen athletes' muscles, but they can interfere with the natural hormone balance in athletes and cause some serious side effects. In men, these side effects include personality changes, abnormal kidney function, breast enlargement, and early baldness; in women, muscle growth, menstrual disorders, and excessive body hair growth. In this experiment, the mouse recombinant androgen receptor active domain protein androgen Receptor (purchased from panvera company, USA), which is highly homologous to the human receptor, will be made into...

Embodiment 1

[0050] 2. Detection: Drop 20-100 μl of the sample to be tested on the protein spotting area, add 2 times the volume of the test solution, which contains the fluorescent labeling ligand Fluormone TM AL 2nM, let stand at room temperature for 30min; add the same volume of normal serum and test reagent to another control area. Treated with the same conditions. After the reaction, wash with Tris washing buffer for 3 times, each time for 2 minutes, measure the fluorescence intensity of each point at the wavelength of ex=485nm, em=535nm, and make a statistical test to determine whether it is positive. The minimum detection amount of the sample is 6pg / ml . The stimulant content in the sample is calculated according to the detection method and formula of the implementation example 1. (Note: The receptor dilution buffer, reaction binding buffer, blocking solution, washing solution, and detection observation solution used refer to the classic androgen receptor competition binding assa...

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Abstract

The present invention provides relative reagent and inspection chip. The inspection method utilizes specificity integration character of excitant receptor protein to excitant molecule to fix relative receptor on surface of solid phase carrier so that excitant composition in test sample can be inspected out for quick screening of tested objects through competition action of excitant molecule and labelled excitant molecule on the receptor.

Description

technical field [0001] The invention relates to a rapid detection biochip for stimulants and a detection method thereof. Background technique [0002] Biochip mainly refers to microanalysis units and systems constructed on the surface of solid chips through microfabrication technology and supramolecular self-assembly technology. Biochips can integrate many different functional devices, such as pretreatment of biological samples, extraction of genetic material, amplification of specific gene fragments, biological probe arrays, and capillary electrophoresis to form an overall microfluidic system to realize the detection of compounds. Accurate, rapid, and informative screening or detection of proteins, nucleic acids, cells, and other biological components. Microarray chip is the most important type of biochip, which integrates a large number of densely arranged biomolecular probes, and can analyze a large amount of molecular information in a short time. Biochips are of great ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64G01N33/50G01N33/58G01N33/68G01N33/74
Inventor 杜冠华陆祖宏周勇殷红唐祖明
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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