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Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction

A radiation-resistant coccus radiation-induced technology, applied in the direction of oxidoreductase, biochemical equipment and methods, enzymes, etc., can solve the problem of the danger of SOD with pathogens, etc., and achieve short fermentation and culture period, low cost and simple process flow Effect

Inactive Publication Date: 2003-10-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the SOD currently on the market uses animal blood as the raw material, and SOD from animal sources may carry the danger of pathogens, such as the mad cow disease that is prevalent in Europe

Method used

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  • Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction
  • Method for increasing yield of superoxide dismutase of radioresistant coccus by radiation induction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Taking a dose of 1000Gy radiation to induce 1L bacterial cell culture and recovering culture for 30 minutes as an example.

[0026] 1 Streak-inoculate radiodurans Dradiodurans R1 on TGY solid medium, incubate at 32°C for 45 hours, pick a single colony and inoculate it in 100ml culture solution, and culture it in a shaker flask for 30 hours. The growth of the bacteria reaches the logarithmic phase (OD 600 >1.0). The liquid seeds were inserted into 1L of fermentation medium according to the inoculum amount of 5%, and cultured at 32° C. with a stirring rotation speed of 200 rpm. Cultivate for 42 hours until the colony count > 10 8 / ml.

[0027] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, the dose rate of 1000Gy / h is irradiated for 1 hour, and the total dose of the bacteria receiving irradiation treatment is 1000Gy.

[0028] 3. The bacteria cells induced by radiation were pla...

Embodiment 2

[0030] Example 2: Take 200Gy dose of radiation to induce 1L bacterial cell culture, and resume culture for 30 minutes as an example.

[0031] 1 Streak-inoculate radiodurans D.radiodurans R1 on TGY solid medium, culture at 30°C for 48 hours, pick a single colony and inoculate it in a conical flask containing 100ml of culture solution, shake the flask for 30 hours, and the growth of the bacteria reaches the desired level. Phases (OD 600 >1.0). The liquid seeds were inserted into 1L of fermentation medium according to the inoculum amount of 5%, and cultured at 32° C. with a stirring rotation speed of 200 rpm. Cultivate for 40 hours until the colony count > 10 8 / ml.

[0032] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, a dose rate of 1000Gy / h was irradiated for 12 minutes, and the total dose of the bacteria received irradiation treatment was 200Gy.

[0033] 3. The bacteria cells induced by ra...

Embodiment 3

[0035] Example 3: Take 500Gy dose of radiation to induce 1L bacterial cell culture, and resume culture for 60 minutes as an example.

[0036] 1 Streak inoculation of radiodurans D.radiodurans R1 on TGY solid medium, culture at 32°C for 48 hours, pick a single colony and inoculate it in a triangular flask containing 100ml of culture solution, shake the flask for 30 hours, and the growth of the bacteria reaches the logarithmic phase (OD 600 >1.0). The liquid seeds were inserted into 1L fermentation medium according to the inoculum amount of 3%, cultured at 32°C, and the stirring speed was 220rpm. Cultivate for 38 hours until the colony count > 10 8 / ml.

[0037] 2 Divide 1L of bacterial culture into polyethylene tubes (γ-rays can penetrate), and place in Co 60 Under radiation irradiation, a dose rate of 1000Gy / h was irradiated for 30 minutes, and the total dose of the bacteria received irradiation treatment was 500Gy.

[0038] 3. The bacteria cells induced by radiation were...

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Abstract

A radiation including method for increasing the output of SOD of the radiation-resistant cocci includes such steps as fermenting culture to obtain stable early radiation-resistant cocci, gamma-ray radiating, and post-culturing. Its advantages are high output rate and activity of SOD, and low cost.

Description

technical field [0001] The invention relates to a method for increasing the production of superoxide dismutase of radioresistant coccus by radiation induction. Background technique [0002] Superoxide dismutase (SOD) is an important antioxidant enzyme in organisms. The physiological function of SOD is to generate hydrogen peroxide and oxygen molecules through the disproportionation reaction of superoxide anion free radicals to prevent superoxide anion free radicals o 2 - Oxidative damage to biological macromolecules in the body. SOD is mainly used in medicine, health care products and cosmetics such as delaying human aging, preventing pigmentation caused by ultraviolet radiation, and removing local inflammation. [0003] At present, there are three main sources of SOD preparation: ① superoxide dismutase in animals (cattle and horse blood); ② SOD from plants (such as tea, cabbage); ③ SOD from microorganisms. Most of the SOD currently on the market...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/02C12N13/00
Inventor 华跃进田兵
Owner ZHEJIANG UNIV
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