Idiosyncratic primer of repetitive sequence in gamma chromosome of oxen, and PRC method for identifying sex in early embryos of oxen

Abstract

A primer specific to the repetitive sequence of ox Y-chromosome has a sequence 5'-TGATTGTTGATCCCACAGAAGG-3',5'-TGAACTTTCAAGCAGOTGAGG-3'. In multiple PCR test, said specific primer and internal standard primer are used to amplify 131 bp specific fragment of Y-chromosome and 250 bp fregment of internal standard gene for bull or only 250 bp fragment of internal standard gene for cow, so it can be used to determine the sex of early ox embryo.

Description

Technical field [0001] The invention relates to the field of molecular biology, in particular to a specific primer sequence of a bovine Y-chromosome repeat sequence for sex identification of bovine early embryos by PCR. Background technique [0002] In the field of agricultural biotechnology, the control of the sex of livestock has been a long-cherished wish for many years. Because through gender control, certain sex-restricted traits (such as cow lactation) and gender-affected production traits (meat production, wool production) can obtain greater economic benefits in more ideal sexes. Increase the intensity of selection in breeding to obtain maximum genetic progress, while eliminating harmful genes in livestock and poultry groups. From the perspective of research development, gender control methods are basically divided into two aspects: one is the separation technology of X and Y sperm, combined with artificial insemination, to control the sex of the offspring. So far, no sati...

AI Technical Summary

Problems solved by technology

Because the core sequence of the SRY gene has high homology (more than 80%) among mammals, such as 84% ​​homology with the core sequence of the SRY gene of people, so when carrying out sex identification of bovine embryos, the male operator The DNA of humans and other animals is likely to contaminate the identification process, especially when performing PCR operations in the field, due to environmental factors, it is easy to produce false positives, thus affecting the accuracy of embryo sex identification

[0005] my country's patent application (application number: 02111848.5) "Bovine Y-chromosome-specific primers and its application in bovine embryo sex identification", the invention uses nested primers, and its nested PCR adopts two-step reactions, and the identification method is relatively complicated. The embryos used still need to extract DNA, so it takes a long time and is very inconvenient

Images