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Method for improving stability of nano material

A technology of nanomaterials and stability, which is applied in the fields of nanoscience, protein and microbiology, and can solve problems such as long synthesis cycle, high environmental risk, and poor stability of nano-selenium

Pending Publication Date: 2022-07-26
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chemically synthesized nano-selenium is easy to synthesize and has controllable particle size, but its stability is poor, the conversion rate is low, and the environmental risk is high; biological nano-selenium has high stability, high biotransformation rate, and low environmental risk, but its synthesis cycle Long, the process is more complicated, the particle size and uniformity are related to the strain, and it is not easy to control
Although there have been many reports on nano-selenium, there is a lack of in-depth research on the stability of nano-selenium and how to use the stabilization mechanism of high-stability biological nano-selenium to solve the poor stability of chemically synthesized nano-selenium

Method used

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  • Method for improving stability of nano material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Separation and characterization of biological nano-selenium

[0063] 1. Synthesis and purification of biological nano-selenium

[0064] The method for synthesizing biological nano-selenium by Lahenia aquatica HX2 refers to Chinese patent CN104774875B. Bio-nano selenium with particle sizes of 260, 146, 118, 90 and 52 nm was prepared from the prepared biological nano-selenium by density gradient centrifugation. The particle size, particle size distribution and polydispersity index of nano-selenium were determined by dynamic light scattering method, and the results are shown in Table 1. The particle morphology was observed by transmission electron microscopy, such as figure 1 As shown in A-E, the bio-nano selenium with different particle sizes are spherical, and the particle size observed by transmission electron microscope is consistent with the results determined by dynamic light scattering method.

[0065] Table 1

[0066]

[0067] 2. Preparation of chemi...

Embodiment 2

[0074] Example 2 HX2 strain synthetic biological nano selenium has strong stability

[0075] 1. The stability of nano-selenium synthesized by HX2 under salt ions

[0076] Final concentration of metal ion salt solution: 1mM, 10mM, 100mM, 1000mM NaCl, 0.1mM, 1mM, 10mM, 100mM MgCl 2 , 0.1mM, 1mM, 10mM, 100mM CaCl 2 , 0.001mM, 0.01mM, 0.1mM, 1mM, 10mM, 100mM AlCl 3 . Take a 96-well plate and add 100 μL of NaCl and MgCl of the corresponding concentrations respectively. 2 , CaCl 2 , AlCl 3 ; Add 100 μL of 2mM biological and chemically synthesized nano-selenium solution to the corresponding salt solution wells, each treatment was repeated three times, and treated at 25 °C for 1 h; use a full-wavelength scanner to read the extinction value at 308 nm wavelength; Calculate the control sample and treatment The ratio of the extinction value of the sample at 308nm wavelength was used to quantify the aggregation degree of chemically synthesized nano-selenium and biological nano-seleni...

Embodiment 3

[0104] Example 3 Stripping and mass spectrometry identification of surface-specific proteins of biological nano-selenium

[0105] 1. Stripping of biological nano-selenium-coated proteins

[0106] Urea treatment strips biological nano-selenium-coated proteins. Take 40 mg of purified bio-nano selenium, treat the bio-nano selenium sample with 7M urea solution, 100 ℃ water bath for 5 min, centrifuge at 4 ℃ for 20 min after treatment, take the centrifuged supernatant, and use polyacrylamide gel electrophoresis (SDS-PAGE) to detect the protein peeling effect. The result is as Image 6 As shown, two protein bands could be separated from the purified bio-nano-Se surface after treatment with 7M urea.

[0107] SDS treatment strips the bio-nano-selenium-coated proteins. Take 40 mg of purified bio-nano-selenium, and treat the bio-nano-selenium samples with 4% SDS urea solution in a water bath at 100 °C for 5 min. After treatment, centrifuge at 4 °C for 20 min. Detection of protein st...

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Abstract

The invention provides a method for improving the stability of a nano material. Flagellin FilC and pore protein OmpF from Rahnella aquatilis and Escherichia coli are used for improving the stability of the nano-material, and the nano-material can be selected from nano-selenium, nano-gold and nano-silver. Compared with other nano-material stabilizers (such as SDS, PEG, PVP, BSA and escherichia coli FilC and OmpF), the stability efficiency of the required Rahnella aquatica FilC and OmpF is 10-100 times that of PVP, BSA and escherichia coli FilC and OmpF under the condition that stable chemical synthesis of nano-selenium is not affected by strong acid, strong alkali and strong ion concentration. The rahnella aquatica FliC and OmpF can also significantly improve the stability of the nano-gold and nano-silver under the condition of strong ion concentration, and the effect is significantly superior to that of escherichia coli FilC and OmpF.

Description

technical field [0001] The invention relates to the fields of microbiology, nanoscience and protein technology, in particular to a method for improving the stability of nanomaterials. Background technique [0002] Selenium (Selenium, Se) is one of the essential trace elements for humans and animals, and is an essential component of thioredoxase, deiodinase, glutathione peroxidase and other selenoenzyme proteins in living organisms. Involved in various metabolic pathways in the human body. Studies have found that selenium deficiency in the human body can lead to a variety of diseases and increase the risk of cancer. my country is rich in selenium resources, but the distribution of selenium in nature is extremely uneven, resulting in selenium deficiency in more than two-thirds of our country. The Chinese Nutrition Society and the FAO / WHO / IAEA Joint Expert Committee have determined that the appropriate range of human intake is 60-250 μg / d, the safe dose is 400 μg / d, and the t...

Claims

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Application Information

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IPC IPC(8): C12P3/00C07K14/195C07K14/245C12R1/01
CPCC12P3/00C07K14/195C07K14/245
Inventor 郭岩彬李奎赵桂慎徐巧林高珊珊
Owner CHINA AGRI UNIV
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