Selective detection of organophosphorus pesticide in food based on orange carbon dot fluorescence method
A technology of organophosphorus pesticides and carbon dot fluorescence, which is applied in the fields of fluorescence/phosphorescence, resistance to vector-borne diseases, and material excitation analysis. The effect of simplifying the sensing process
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Embodiment 1
[0028] Example 1: Synthesis and characterization of CDs@CoOOH NFs fluorescent composites
[0029] First, nitrogen-rich CDs were synthesized by a one-step solvothermal reaction of urea and citric acid in the presence of N,N-dimethylformamide. The morphological characteristics of CDs were observed by transmission electron microscopy (TEM), and the results were as follows figure 1 As shown in a, the CDs have spherical structures with an average diameter of 4.0 nm. High-resolution transmission electron microscopy (HRTEM) revealed that the CDs had a highly crystalline structure with lattice fringes of 0.21 nm, consistent with the (100) plane of graphite. The X-ray diffraction (XRD) pattern shows a sharp peak at 27.5°, which is related to the characteristic diffraction peak (002) of graphite, confirming the existence of a highly conjugated sp2 domain in the core part of the CDs ( figure 1 b). Under the excitation condition of 510 nm, CDs showed the maximum fluorescence emission i...
Embodiment 2
[0030] Example 2: Determination of the mechanism of CDs@CoOOH NFs fluorescence system
[0031] To determine the mechanism of the CDs@CoOOH NFs fluorescence system, we carried out systematic electronic and spectroscopic tests. Surface charge analysis of CDs and CoOOH NFs by zeta potential measurements ( figure 2 a), in which negatively charged CDs (ζ=-15.3mV) can be electrostatically immobilized on the surface of CoOOH NFs (ζ=+8.7mV) to form CDs@CoOOH composites (ζ=-10.5mV). The successful synthesis of the composites was further verified by measuring the UV-Vis absorption spectra of CoOOH NFs in the presence of CDs. The results showed that the characteristic absorption peaks of the composites were blue-shifted by 10 nm compared with free CoOOH NFs ( figure 1 e), while the absorption spectra of CoOOH NFs overlapped with the fluorescence emission spectra of CDs ( figure 2 b), CoOOH NFs can act as energy acceptors to absorb the energy of CDs, resulting in rapid quenching of fl...
Embodiment 3
[0036] Example 3: Sensitive and specific detection of paraoxon
[0037] The AChE / ATCh / CDs / CoOOH platform designed in the present invention has good performance and can be used as a candidate platform for monitoring OPs (enzyme inhibitors). Paraoxon is a typical inhibitor that blocks the catalytic activity of acetylcholinesterase, which can make The fluorescence of the system changes. In this example, the influence of factors such as pH, reaction temperature and incubation time on the detection sensitivity was systematically considered, and the conditions for the established platform to achieve the best performance were discussed, such as Figure 4 As shown in a, the pH to fluorescence ratio (F / F 0 ) has a significant effect, when the pH rises from 6.0 to 7.4, the F / F of the system 0 gradually increased, and when the pH was further increased to 8.5, the F / F of the system 0 Therefore, pH 7.4 Tris-HCI buffer was selected for the detection of paraoxon; meanwhile, the effect of ...
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