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Selective detection of organophosphorus pesticide in food based on orange carbon dot fluorescence method

A technology of organophosphorus pesticides and carbon dot fluorescence, which is applied in the fields of fluorescence/phosphorescence, resistance to vector-borne diseases, and material excitation analysis. The effect of simplifying the sensing process

Pending Publication Date: 2022-07-12
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various methods, including chromatography, mass spectrometry, and hyphenated methods, have been designed to detect organophosphorus pesticide residues in products. Although these methods can accurately obtain the concentration of OPs, they are time-consuming, complex instruments, and poor availability in the field. Limit their use for simple, rapid and sensitive detection of organophosphorus pesticides, especially in emergency situations

Method used

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  • Selective detection of organophosphorus pesticide in food based on orange carbon dot fluorescence method
  • Selective detection of organophosphorus pesticide in food based on orange carbon dot fluorescence method
  • Selective detection of organophosphorus pesticide in food based on orange carbon dot fluorescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Synthesis and characterization of CDs@CoOOH NFs fluorescent composites

[0029] First, nitrogen-rich CDs were synthesized by a one-step solvothermal reaction of urea and citric acid in the presence of N,N-dimethylformamide. The morphological characteristics of CDs were observed by transmission electron microscopy (TEM), and the results were as follows figure 1 As shown in a, the CDs have spherical structures with an average diameter of 4.0 nm. High-resolution transmission electron microscopy (HRTEM) revealed that the CDs had a highly crystalline structure with lattice fringes of 0.21 nm, consistent with the (100) plane of graphite. The X-ray diffraction (XRD) pattern shows a sharp peak at 27.5°, which is related to the characteristic diffraction peak (002) of graphite, confirming the existence of a highly conjugated sp2 domain in the core part of the CDs ( figure 1 b). Under the excitation condition of 510 nm, CDs showed the maximum fluorescence emission i...

Embodiment 2

[0030] Example 2: Determination of the mechanism of CDs@CoOOH NFs fluorescence system

[0031] To determine the mechanism of the CDs@CoOOH NFs fluorescence system, we carried out systematic electronic and spectroscopic tests. Surface charge analysis of CDs and CoOOH NFs by zeta potential measurements ( figure 2 a), in which negatively charged CDs (ζ=-15.3mV) can be electrostatically immobilized on the surface of CoOOH NFs (ζ=+8.7mV) to form CDs@CoOOH composites (ζ=-10.5mV). The successful synthesis of the composites was further verified by measuring the UV-Vis absorption spectra of CoOOH NFs in the presence of CDs. The results showed that the characteristic absorption peaks of the composites were blue-shifted by 10 nm compared with free CoOOH NFs ( figure 1 e), while the absorption spectra of CoOOH NFs overlapped with the fluorescence emission spectra of CDs ( figure 2 b), CoOOH NFs can act as energy acceptors to absorb the energy of CDs, resulting in rapid quenching of fl...

Embodiment 3

[0036] Example 3: Sensitive and specific detection of paraoxon

[0037] The AChE / ATCh / CDs / CoOOH platform designed in the present invention has good performance and can be used as a candidate platform for monitoring OPs (enzyme inhibitors). Paraoxon is a typical inhibitor that blocks the catalytic activity of acetylcholinesterase, which can make The fluorescence of the system changes. In this example, the influence of factors such as pH, reaction temperature and incubation time on the detection sensitivity was systematically considered, and the conditions for the established platform to achieve the best performance were discussed, such as Figure 4 As shown in a, the pH to fluorescence ratio (F / F 0 ) has a significant effect, when the pH rises from 6.0 to 7.4, the F / F of the system 0 gradually increased, and when the pH was further increased to 8.5, the F / F of the system 0 Therefore, pH 7.4 Tris-HCI buffer was selected for the detection of paraoxon; meanwhile, the effect of ...

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Abstract

The invention discloses a method for selectively detecting organophosphorus pesticides in food based on an orange carbon dot fluorescence method. In the invention, the orange fluorescence emission CDs is used as an amplification detection signal, so that the influence of the fluorescence of the food matrix can be overcome, the background interference is effectively blocked, and the detection reliability is improved; coOOH NFs is used as a fluorescence quencher and can be used for efficiently quenching CDs fluorescence; the fluorescent response of the system is regulated and controlled by combining the mechanism that acetylcholin esterase (AChE) specifically catalyzes thioacetylcholine to inhibit the fluorescence quenching effect of CoOOH NFs and organophosphorus OPs specifically inhibits the activity of AChE, paraoxon can be rapidly detected, and the detection limit is 0.03 ngmL <-1 >. A fluorescence detection system constructed by the method is combined with a hydrogel kit, and a portable platform for quickly monitoring oxygen and phosphorus on site is prepared by utilizing a high-definition camera shooting function of a smart phone, so that a new visual angle is provided for accurately monitoring food safety on site.

Description

technical field [0001] The invention belongs to the fields of biosensor technology and food quality detection, and in particular relates to a preparation method of an orange fluorescent carbon dot self-assembled on a cobalt oxyhydroxide nanosheet fluorescent composite material (CDs@CoOOH NFs), based on the composite (CDs@CoOOH) To control the reaction of acetylcholinesterase (AChE), a reliable and high-sensitivity fluorescence detection strategy for organophosphorus pesticides (OPs) was established, and a portable platform combining the fluorescence detection system and the hydrogel kit was cleverly constructed. , which provides a new perspective for on-site monitoring of organophosphorus pesticides using smartphones. Background technique [0002] Organophosphorus pesticides (OPs) play a crucial role in crop growth and storage. However, organophosphorus pesticides will pose a huge threat to human health even at low concentrations. Therefore, many countries and regions have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432Y02A50/30
Inventor 李红霞苏长顺吕婷邹睿琦刘妮杨川宇孙春燕
Owner JILIN UNIV
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