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Chip, method for preparing chip and application of chip

A chip and molecule technology, applied in the field of biochemistry, can solve the problem that the chip needs to be improved, and achieve the effect of avoiding the reduction of synthesis efficiency, reducing the error rate and improving the synthesis efficiency.

Pending Publication Date: 2022-07-01
SHENZHEN HUADA GENE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, current chips for synthesizing biomacromolecules still need to be improved

Method used

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  • Chip, method for preparing chip and application of chip
  • Chip, method for preparing chip and application of chip
  • Chip, method for preparing chip and application of chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: Chip modification with Boc-7-aminoheptanoic acid as spacer molecule

[0088] The process of chip modification such as figure 2 shown, the specific steps are as follows:

[0089] 1. Put the QR code bare chip (2*2*0.45mm) in a 50mL centrifuge tube, add 25mL deionized water, cover with a sealing film, ultrasonically shake for 10min, use deionized water for three times, and acetone for three times , and then placed in an oven at 75 °C for 10 min.

[0090] 2. Prepare 10 mL of 1% silanization reagent (APTES:PTES=1:1), put in 200 cleaned QR code chips, sonicate for 45 min in an ultrasonic machine, wash with acetone for 5 times, dry and place at 75°C Oven dry for 10 min.

[0091] 3. Take 82mg Boc-7-aminoheptanoic acid, 120mg 2-(7-azabenzotriazole)-N,N,N',N'-tetramethylurea hexafluorophosphate (HATU) in 15mL In a centrifuge tube, add 300 μL N,N-diisopropylethylamine (DIPEA) and 15 mL acetonitrile, shake well, put in 200 silanized QR code chips, and sonicate for 4...

Embodiment 2

[0098] Example 2: Chip modification without spacer molecules

[0099] The process of chip modification such as image 3 shown, the specific steps are as follows:

[0100] 1. Place the QR code bare chip (2*2*0.45mm) in a 50mL centrifuge tube, add 50mL deionized water, cover with sealing film, ultrasonically shake for 15min, use deionized water for three times, and acetone for three times , and then placed in an oven at 75 °C for 10 min.

[0101] 2. Take 200 mL of acetone, 1000 μL of APTES, and 1000 μL of PTES, add them to the fresh-keeping box in sequence, divide them into two boxes, and mix each box to make 100 ml of 1% silanization reagent. Transfer the dried 5000 chips to crisper boxes, 2500 chips per box. Add 100 mL of silanization reagent, cover tightly, and sonicate for 45 min in a sonicator. After the reaction was completed, it was transferred to a 50 mL centrifuge tube, the solution was drained, washed with acetone for 5 times, dried in the air, and then placed in a...

Embodiment 3

[0105] Example 3: Chip modification with succinic anhydride-hexanediamine as spacer molecule

[0106] The process of chip modification such as Figure 4 shown, the specific steps are as follows:

[0107] 1. Put the QR code bare chip (2*2*0.45mm) in a 50mL centrifuge tube, add 25mL deionized water, cover with a sealing film, ultrasonically shake for 10min, use deionized water for three times, and acetone for three times , to dry.

[0108] 2. Prepare 5mL of 1% silanization reagent (APTES:PTES=1:1), put in 150 cleaned QR code chips, sonicate for 45min in an ultrasonic machine, wash with acetone for 5 times, dry and place at 75°C Oven dry for 10 min.

[0109] 3. Dissolve 17 mg of succinic anhydride in 3 mL of DMF, shake well, put in 150 pieces of silanized two-dimensional code chips, and stir for 24 hours with a vertical stirrer. After the reaction was completed, the chips were collected, washed three times with ethanol and acetone respectively, dried in the air, and then plac...

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Abstract

The invention provides a chip which comprises a substrate, the substrate is connected with a connecting molecule, one end, far away from the substrate, of the connecting molecule carries a hydroxyl group, the hydroxyl group is protected by an acid unstable group, the connecting molecule is connected with the substrate through an initial molecule and a spacer molecule, the starting molecule is connected with the substrate, the spacer molecule and the starting molecule form covalent linkage, one end of the connecting molecule is connected with one end, far away from the starting molecule, of the spacer molecule, and the spacer molecule comprises at least one monomer selected from amino heptanoic acid, succinic anhydride-hexamethylenediamine and TESHBA. The modified molecules on the surface of the chip are uniformly distributed, the requirement of subsequent biomacromolecule synthesis can be ensured, and the preparation method of the chip is simple, short in steps and beneficial to large-scale production.

Description

technical field [0001] The present invention relates to the field of biochemistry, in particular, to a chip and its preparation method and use. Background technique [0002] Biochip, also known as protein chip or gene chip, usually referred to as chip, generally refers to the high density of biological information molecules (such as gene fragments, DNA fragments or polypeptides, proteins, sugar molecules, tissues, etc.) Micro-array hybrid chip (micro-arrays), the sequence and position of each molecule in the array are known, and it is a pre-set sequence lattice, which is based on the principle of specific interaction between biomolecules. The analysis process is integrated on the chip surface, enabling high-throughput and rapid detection of DNA, RNA, peptides, proteins, and other biological components. Biochips have the characteristics of high throughput, high sensitivity and high integration, so they are widely used in molecular biology, biomedicine, drug research and deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/5027
Inventor 王勇何晨菲江湘儿林玉彬原超峰沈玥
Owner SHENZHEN HUADA GENE INST
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