Method for increasing tropane alkaloid content in belladonna hairy roots by using calcium signal inhibitor
An inhibitor and calcium signal technology, applied in the field of plant biology, can solve the difficult problems of TAs synthesis regulation mechanism to provide access, reduce, increase the total content of TAs, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0057] 1. Calcium treatment of belladonna hair roots
[0058] Belladonna hair roots were expanded and cultured in liquid medium to prepare for treatment, and were subcultured twice on MS solid medium. After the first subculture 7-10 days, the newly grown tender white and The multi-branched hair roots were subcultured for the second time. After about 5-7 days, the physiological state and growth of the root tips of the hair roots were basically the same. Cut three root tips with a length of 3-4 cm and inoculate them in 50 mL of MS liquid medium at 110 rpm. , 25 ℃ shaker dark culture for subsequent processing.
[0059] Calcium treatment: hair roots were inoculated with different CaCl 2 Concentration of calcium treatment medium, harvested after culturing for 25 days, the medium was extracted with alkaloids, and the roots were dried at 40°C and ground into powder to prepare alkaloids;
[0060] Treatment with various calcium signal inhibitory reagents: Remove the old medium from h...
PUM
Property | Measurement | Unit |
---|---|---|
Wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com