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Application of phycofuscin to preparation of composition for preventing, treating or improving heart diseases

A heart disease and composition technology, applied in metabolic diseases, drug combinations, medical preparations containing active ingredients, etc., can solve problems such as valve damage, cell apoptosis, and heart valve calcification

Pending Publication Date: 2022-05-27
台湾中华海洋生技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Normally, healthy valve cells are predominantly of the fibroblast type, but apoptosis is induced in the presence of increased reactive oxygen species, and oxidative stress can promote heart valve calcification and lead to valve damage

Method used

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  • Application of phycofuscin to preparation of composition for preventing, treating or improving heart diseases
  • Application of phycofuscin to preparation of composition for preventing, treating or improving heart diseases
  • Application of phycofuscin to preparation of composition for preventing, treating or improving heart diseases

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0049] Preparation Example 1 Isolation and purification of primary rat heart valve interstitial cells (VIC)

[0050] According to Lin C., Zhu D., Markby G., Corcoran B.M., Farquharson C., Macrae V.E. Isolation and Characterization of Primary Rat Valve Interstitial Cells: ANew Model to Study Aortic Valve Calcification.J.Vis.Exp.2017: 56126. Isolation and purification of rat heart valve interstitial cells, in short, after collecting all leaflets, centrifugation and co-action with collagenase II (collagenase II) for 2 hours to obtain heart valve interstitial fragments (debris). The recipients will obtain rat heart valve interstitial cells containing 10% fetal bovine serum (Fetal Bovine Serum, FBS; CORNING, Manassas, VA, USA), 100 units / mL penicillin, 100 μg / mL streptomycin, 2.438g Dulbecco's Modified Eagle Medium (DMEM) / F12 medium (CASSION, Taichung, Taiwan, China) in sodium bicarbonate / L sodium bicarbonate was cultured at 37°C in a humidified 5% carbon dioxide environment for 2...

Embodiment 1VI

[0051] Example 1 Cell viability test of VIC cells

[0052] The VIC cells of Preparation Example 1 were seeded on a 96-well plate, and the cell density was 3000 cells per well. After waiting for 24 hours for the cells to attach, the following groups were respectively treated: (1) The concentration was 0.1mM and 0.2mM. , 0.5mM, 1mM, 10mM H 2 O 2 Treated for 15 minutes, 1 hour, 4 hours; (2) treated with 0.01 mg / mL (mg / mL), 0.1 mg / mL, 0.5 mg / mL, 1 mg / mL, 5 mg / mL phycoxanthin aqueous solution for 24 hours, 48 hours, 72 hours; (3) treated with 0.01 mg / mL (mg / mL), 0.1 mg / mL, 0.5 mg / mL, 1 mg / mL aqueous solution of phycoxanthin for 24 hours and then treated with 0.5 mM H 2 O 2 Process for 15 minutes. Next, the medium was removed after incubation with serum-free medium containing 1 mg / mL MTT reagent for 3 hours. Armor again (formazan) crystals were dissolved in 100 μL of dimethyl sulfoxide (DMSO) per well, and then read at 570 nm (nm) and absorbance at 630 nm with a microplate an...

Embodiment 2

[0054] Example 2 Cell Counting

[0055] The VIC cells of Preparation Example 1 were seeded on a 96-well plate, and the cell density was 3000 cells per well, and the cells were allowed to attach after waiting for 24 hours. First, it was treated with 0.01 mg / mL, 0.1 mg / mL, 0.5 mg / mL, and 1 mg / mL phycoxanthin aqueous solutions for 24 hours, and then treated with 0.5 mM hydrogen peroxide for 15 minutes. Next, cells were detached with trypsin, and the cell suspension was mixed with a 0.4% trypan solution. Cell numbers were counted at 200X magnification using a hemocytometer (Hausser scientific company, Horsham, PA, USA) and an inverted phase-contrast microscope.

[0056] The result is as figure 2 shown, H 2 O 2 Treatment causes cell death, while phycoxanthin can alleviate H 2 O 2 Treatment resulted in growth inhibition of VIC cells.

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Abstract

The invention provides application of phycofuscin to preparation of a composition for preventing, treating or improving heart diseases. The invention proves that the phycofuscin can relieve growth inhibition of rat primary cardiac valvular interstitial cells caused by H2O2, avoid cell damage and DNA damage of valvular interstitial cells caused by oxidation pressure, reduce the density of active oxygen, recover the apoptosis phenomenon caused by H2O2 and relieve valvular interstitial cell calcification caused by H2O2. And mitral valve leakage, tricuspid valve leakage and compensatory cardiac expansion of dogs in animal experiments are improved.

Description

technical field [0001] The present invention relates to the use of a phycoxanthin, in particular to the use of a pharmaceutical composition or a food composition for preventing, treating or improving heart disease. Background technique [0002] The pathogenesis of cardiovascular disease, such as atherosclerosis and aortic valve sclerosis, involves inflammatory responses to various stimuli, including high levels of low-density lipoprotein (LDL) and reactive oxygen species (reactive oxygen species, ROS). Reactive oxygen species are caused by increased oxidative stress, infection and chemical damage. In cardiovascular disease, increased myocardial cell load or insufficient energy supply can lead to an imbalance of energy supply and demand, resulting in increased energy metabolism and mitochondrial redox. This ultimately leads to increased reactive oxygen species and damage to heart cells. [0003] Heart valves, such as the aortic valve, mainly include two types of cells: val...

Claims

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Application Information

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IPC IPC(8): A61K31/336A61P3/00A23L33/105
CPCA61K31/336A61P3/00A23L33/105A23V2002/00A23V2250/202A23V2200/30
Inventor 夏诗闵蔡志鸿
Owner 台湾中华海洋生技股份有限公司
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