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A rapid detection method for amitraz residues in agricultural products

A detection method, the technology of amitraz, which is applied in the field of rapid detection of amitraz residues in agricultural products, can solve the problems of high difficulty in the synthesis of calixarene, differences in hydrolyzed product dimethylaniline, obvious limitations, etc., and achieve correct detection results High rate, intuitive detection results, simple and easy-to-understand principle

Active Publication Date: 2022-06-28
广东江门中医药职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above three methods are all effective methods for detection, but their limitations are more obvious
The first method uses chromatography. This method is difficult to operate, the processing steps are cumbersome, it takes a long time, the method has a low error tolerance rate, and has high requirements for operators.
The second method, although the operation is simpler than the first method, the amount of the hydrolyzed product dimethylaniline will form a large difference due to the influence of the hydrolysis conditions. The research is only based on the data introduced in the literature, which is relatively one-sided. The result is far lower than expected, and the low recovery rate is a difficult problem for the second method
The third method is not a commonly used detection method in the laboratory. The synthesis of calixarene is also relatively difficult, which increases the cost of the method and greatly reduces the practicability. It is only suitable for research rather than actual detection scenarios.
None of the above methods is suitable for on-site rapid analysis and detection, and cannot meet the growing detection needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this example, chloroform is selected as analytically pure chloroform supplied by Sinopharm Group Chemical Reagent Co., Ltd., anhydrous sodium sulfate is selected as 99% anhydrous sodium sulfate supplied by Sinopharm Group Chemical Reagent Co., Ltd., and m-xylene is selected from Sinopharm Group Chemical Reagent Co., Ltd. The analytically pure m-xylene supplied, ethanol is selected from the analytical pure ethanol supplied by Sinopharm Group Chemical Reagent Co., Ltd., phthalic anhydride is selected from the analytically pure phthalic anhydride supplied by Sinopharm Group Chemical Reagent Co., Ltd., and hydrazine hydrate is selected from Sinopharm Group Chemical The 98% synthetic hydrazine hydrate supplied by Reagent Co., Ltd., the hydrochloric acid solution with the mass fraction of 36%~38% supplied by Sinopharm Chemical Reagent Co., Ltd. is selected for hydrochloric acid; Sodium sulfate water, glacial acetic acid select analytically pure glacial acetic acid supplied ...

Embodiment 2

[0054] A kind of rapid detection method of amphetamine residues in agricultural products of the present invention comprises the following:

[0055] Step a, weigh 100 g of the vegetable sample to be tested from which the sludge has been removed, in a homogenizer, and homogenize to obtain a homogeneous vegetable sample to be tested;

[0056] Step b: Weigh 5g of the homogeneous vegetable sample to be tested in a 50ml first centrifuge tube, add 10ml of phosphate buffer solution with a pH value of 6.8, shake and mix for 1min, add 5ml of a mixed solution of chloroform and m-xylene, and shake to extract 3min, centrifuge at 4000 prm / min for 1min, take 2ml of the lower layer solution into a 15ml second centrifuge tube, and dry it in the air at 60°C in a fume hood. The volume ratio of chloroform and m-xylene in the mixed solution of chloroform and m-xylene is 9.5: 0.5;

[0057] Step c, add 2ml of ethanol to the second centrifuge tube after drying, transfer to a 500ml beaker after recon...

Embodiment 3

[0064] A kind of rapid detection method of amphetamine residues in agricultural products of the present invention comprises the following:

[0065] In step a, 100 g of the fruit samples to be tested that have been removed from the sludge are weighed in a homogenizer, and homogenized to obtain a homogeneous fruit sample to be tested;

[0066] Step b: Weigh 10 g of the homogeneous fruit sample to be tested in a 50 ml first centrifuge tube, add 10 ml of a phosphate buffer solution with a pH value of 7.2, shake and mix for 1 min, add 5 ml of a mixed solution of chloroform and m-xylene, and shake to extract 3min, centrifuge at 4000 prm / min for 1min, remove 2.5ml of the lower layer solution into a 15ml second centrifuge tube, dry it in the air at 70°C in a fume hood, the volume ratio of chloroform and m-xylene in the mixed solution of chloroform and m-xylene is 9.5 :0.5;

[0067] Step c, add 2ml of ethanol to the second centrifuge tube after drying, transfer to a 500ml beaker after...

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Abstract

The invention discloses a rapid detection method for amitraz residue in agricultural products, comprising the following steps: step a, preparing a homogeneous sample to be tested; step b, extracting and concentrating; step c, redissolving; step d, adding phthalate The chloroform solution of formic anhydride is subjected to protection reaction; step e, deprotection reaction to generate xylidine; step f, color reaction; step g, result judgment. The principle of the method of the present invention is simple and easy to understand, with strong specificity, high sensitivity, intuitive detection results, high accuracy of detection results, no need for large-scale analysis instruments, relatively less time-consuming, and extremely low average analysis cost, and is specially suitable for agricultural products. The on-site rapid detection of amitraz residues in Chinese medicine has strong practicability.

Description

technical field [0001] The invention relates to the field of agricultural product detection, in particular to a method for rapid detection of amitraz residues in agricultural products. Background technique [0002] Diformamidine, also known as acarid, amphetamidine, and fruit miticide, is a broad-spectrum formamidine acaricidal insecticide, which is a moderately toxic acaricide. Chemical name: 1,5-bis-(2,4-dimethylphenyl)-3-methyl-1,3,5-triazapentadiene-1,4, the original drug is white or yellow needles Crystalline, slightly soluble in water, soluble in various organic solvents such as xylene, acetone and methanol. It is stable to temperature and light under anhydrous conditions and unstable under acidic conditions. The latest national standard "GB / T 2763-2021 Maximum Residue Limits of Pesticides in Foods" requires that the limit value of amphetamine in vegetables and fruits is 0.5mg / kg. [0003] At present, there are mainly the following methods for commercial detection o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N1/40
CPCG01N21/78G01N1/40
Inventor 熊文明李拥军温宝莹莫婷筠陈文浩李斌余廉柱
Owner 广东江门中医药职业学院
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