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Drug-loaded bone repair material as well as preparation method and application thereof

A repair material and bone repair technology, applied in the field of biomedicine, can solve the problems of uncontrollability, increased risk of overgrowth of bone tissue, heterotopic ossification and other complications, and difficulty in accurately regulating the release efficiency of different bioactive factors. Achieve the effect of promoting bone tissue regeneration and good biocompatibility

Inactive Publication Date: 2022-05-06
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing bone repair materials still have deficiencies in the delivery strategy of bioactive factors: 1) The difference in the degradation characteristics of different biomaterials is used to deliver a variety of bioactive factors, which is uncontrollable, and it is still difficult to precisely control different bioactive factors 2) Studies have shown that the late stage of the inflammatory response in the process of bone repair is the best therapeutic window for delivering osteoinductive factors, while the most commonly used slow-release delivery strategy for existing bone repair materials is difficult to precisely regulate osteogenesis The release time and concentration of inducing factors; 3) Bioactive factors, such as bone morphogenetic protein-2 (BMP-2), the efficacy of treatment is highly dependent on the method of delivery to the target site, and the rapid hydration of the carrier after contact with biological tissue Or dissolution may lead to cytokine elution, and to compensate for this loss, cytokines are often administered in high concentrations, further increasing the risk of bone tissue overgrowth, heterotopic ossification, and other complications

Method used

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  • Drug-loaded bone repair material as well as preparation method and application thereof
  • Drug-loaded bone repair material as well as preparation method and application thereof
  • Drug-loaded bone repair material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: a bone repair material

[0057] The preparation method is as follows:

[0058] 1. Preparation of hydroxybutyl chitosan

[0059] The preparation of hydroxybutyl chitosan (HBC) is divided into three steps: alkalization, modification and post-treatment of chitosan:

[0060] (1) Weigh 1g chitosan (sigma, USA) powder and disperse it in 20ml NaOH solution (50%, w / W) for alkalization treatment. Stir at room temperature for 48h, filter and remove the excess alkali solution.

[0061] (2) The alkalized chitosan was added into isopropanol / water (1:1, V / V) solution and dispersed at room temperature for 24 hours until the alkalized chitosan was completely dispersed in isopropanol / water solution. Add 20 ml of 1,2-epoxy butane (Aladdin, China) slowly into the dispersion obtained in the above steps, and place it in a heated magnetic stirrer for reaction at 55 ℃ for 72 hours. After the reaction, cool to room temperature, add 0.1 M HCl solution dropwise to the reaction sol...

Embodiment 2

[0078] Example 2. Detection of physical and chemical properties of bone repair materials

[0079] (1) Morphological observation of bone repair materials

[0080] HBC, HBC + ASP, HBC + 5MP BMP and HBC + ASP + 5MP BMP bone repair materials were freeze-dried with a vacuum freeze dryer. The exposed section of the bone repair materials was cut with a blade and sprayed with gold in vacuum. The section morphology and internal microstructure of the bone repair materials were observed with scanning electron microscope (SEM), and the voltage was 5.0 kV.

[0081] Results such as Figure 3 It is shown that HBC + ASP + 5mp-bmp bone repair material is loose and porous inside, with good morphology, and its pore diameters are 70 ~ 100 μ m. This pore size is conducive to the climbing of cells, the absorption of nutrients and the elimination of metabolic waste. The addition of ASP and 5mp-bmp microspheres had no significant effect on the internal structure of bone repair materials. The internal pore...

Embodiment 3

[0085] Example 3. Detection of stimulation response characteristics and factor release characteristics of bone repair materials

[0086] (1) Detection of temperature sensitive properties of bone repair materials

[0087] The rheological behavior of HBC, HBC + ASP, HBC + 5mp-bmp and HBC + ASP + 5mp-bmp were observed with HAAKE Mars 40 rheometer. The measurement method is as follows: place the colloidal bone repair material on the detection plate with a diameter of 25 mm, set the test distance between the parallel plates as 0.1 mm, the measurement temperature range is 4 ~ 40 ℃, the heating rate is 1 ℃ / min, the fixed strain is 1%, and the frequency is 1 Hz, and record the storage modulus of the bone repair material sample varying with temperature And loss modulus Changes in. During the experiment, silicon oil layer was coated around the bone repair material to prevent the evaporation of water during the heating process.

[0088] Results such as Figure 5 As shown in B, the sol-gel...

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Abstract

The invention discloses a drug-loaded bone repair material as well as a preparation method and application thereof. The drug-loaded bone repair material comprises an osteogenic factor 1, hydroxybutyl chitosan and polydopamine coated magnesium-containing calcium carbonate microspheres loaded with an osteogenic factor 2, and dispersing the polydopamine-coated magnesium-containing calcium carbonate microspheres loaded with the osteogenic factor 2 and hydroxybutyl chitosan into an osteogenic factor 1 solution to obtain the drug-loaded bone repair material, wherein the polydopamine-coated magnesium-containing calcium carbonate microspheres loaded with the osteogenic factor 2 and the hydroxybutyl chitosan are dispersed into the osteogenic factor 1 solution. The drug-loaded bone repair material disclosed by the invention can realize accurate sequence delivery of anti-inflammatory drugs and osteogenic protein after being implanted into a bone defect part, so that the early inflammatory reaction after bone injury is relieved, the formation of new bone tissues is promoted, and the bone regeneration efficiency is improved.

Description

technical field [0001] The application relates to the field of biomedical technology, in particular to a drug loaded bone repair material and its preparation method and application. Background technology [0002] The incidence rate of oral craniomaxillofacial bone defect is high. Its etiology is diverse, its shape is complex, and its bone regeneration is difficult, which seriously affects the quality of life and physical and mental health of patients. Bone injury repair process is a highly complex and dynamic process. At present, bone tissue engineering often uses different kinds of bone repair materials to load and deliver a variety of bioactive factors to act on different stages of bone injury repair to regulate the process of bone regeneration. For example, the sequence delivery of anti-inflammatory factors and osteogenic inducible factors by bone repair materials can effectively regulate the inflammatory response in the early stage of bone injury, which is conducive to the tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/20A61L27/04A61L27/02A61L27/18A61L27/50
CPCA61L27/54A61L27/20A61L27/047A61L27/025A61L27/18A61L27/50A61L2430/02A61L2300/414A61L2300/41A61L2300/216A61L2400/06A61L2300/602C08L5/08C08L79/04
Inventor 周永胜吕珑薇万竹青刘云松张萍张晓董沁媛
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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