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Acellular matrix particle compounded with human mesenchymal stem cell culture supernatant component as well as preparation method and application of acellular matrix particle

A technology of acellular matrix and mesenchymal stem cells, applied in the field of acellular matrix particles compounded with human mesenchymal stem cell culture supernatant and its preparation, can solve the problem of loss of water-soluble cytokines and other issues

Active Publication Date: 2022-04-26
ACADEMY OF MILITARY MEDICAL SCI
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a low-immunogenic acellular matrix particle compounded with human mesenchymal stem cell culture supernatant and its preparation method, to solve The problem of loss of water-soluble cytokines in the preparation process of acellular matrix in the prior art

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  • Acellular matrix particle compounded with human mesenchymal stem cell culture supernatant component as well as preparation method and application of acellular matrix particle
  • Acellular matrix particle compounded with human mesenchymal stem cell culture supernatant component as well as preparation method and application of acellular matrix particle

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Embodiment 1

[0048] Example 1 Preparation of Acellular Matrix Microparticles Composite with Human Mesenchymal Stem Cell Culture Supernatant Components

[0049] 1. Acquisition of concentrated supernatant of mesenchymal stem cell culture

[0050] 1.1. Obtaining human mesenchymal stem cells: (taking umbilical cord mesenchymal stem cells as an example) cut the isolated human umbilical cord into small sections of about 2 cm, cut the umbilical cord longitudinally, peel off and remove the arteries and veins, and collect Wharton's jelly (SWJ), use 0.9% sodium chloride injection to clean up, then cut Wharton's jelly into about 1mm 3 For tissue pieces of the size of 100, take an appropriate amount and put them into a culture dish, spread them evenly, and then add mesenchymal stem cell culture medium ( MSC Proliferation Serum-Free Medium (ME000-N023), Ecosai Biotechnology) at 37°C, 5% CO 2 Culture in an incubator for about 10 days, remove all tissue pieces to obtain primary human mesenchymal stem ...

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Abstract

The invention discloses an acellular matrix particle compounded with a human mesenchymal stem cell culture supernatant component as well as a preparation method and application of the acellular matrix particle. The invention specifically discloses a preparation method of composite acellular matrix particles, which comprises the following steps: mixing human mesenchymal stem cell culture supernatant and acellular matrix particles in proportion, and freeze-drying to obtain the composite acellular matrix particles. Aiming at the problem that the acellular matrix loses cytokines, the acellular dermal matrix is made into acellular dermal microparticles under the action of mechanical force, the concentrated mesenchymal stem cell supernate is added into the acellular dermal microparticles, and then freeze-drying preservation is performed, so that the concentration of the cytokines in the mesenchymal stem cell supernate is improved, and the content of the cytokines in the mesenchymal stem cell supernate is reduced. According to the acellular dermal matrix, the storage life of the acellular dermal matrix is prolonged, the abundance of cytokines of the acellular dermal matrix can be improved, and the acellular dermal matrix is used as a carrier, so that the functions of an extracellular matrix and culture supernatant cytokines are better exerted.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and specifically relates to an acellular matrix particle compounded with human mesenchymal stem cell culture supernatant components, a preparation method and application thereof. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are pluripotent stem cells derived from early mesoderm, which have self-renewal ability and multi-directional differentiation potential. , ligament, nerve, liver, myocardium, endothelium and other tissue cells. Mesenchymal stem cells are mainly derived from human mesenchymal tissues, such as fat, bone marrow, umbilical cord, and placenta. Due to their strong proliferation ability and low immunogenicity, they have attracted more and more attention. They are the seed cells for tissue engineering projects. source. [0003] The culture supernatant of mesenchymal stem cells contains a variety of cytokines. A large number of stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3604A61L27/362A61L27/3629A61L27/3687A61L27/3691A61L27/50A61L2430/34A61L2430/40
Inventor 张士坤宫锋邹昊玉李素波高红伟张雪
Owner ACADEMY OF MILITARY MEDICAL SCI
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