Multiplex PCR detection primer group, kit and detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus

A waterfowl parvovirus and duck enteritis virus technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of limited detection sensitivity, high detection cost, long detection time, etc., and achieve improved High detection efficiency, high sensitivity, and strong specificity

Pending Publication Date: 2022-04-12
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has defects such as long detection time, high detection cost, complicated operation, and limited detection sensitivity.

Method used

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  • Multiplex PCR detection primer group, kit and detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus
  • Multiplex PCR detection primer group, kit and detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus
  • Multiplex PCR detection primer group, kit and detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus

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Embodiment

[0052] The multiplex PCR detection primer set of a kind of water fowl parvovirus, duck enteritis virus and goose astrovirus proposed by the present invention adopts the following technical scheme:

[0053] A multiplex PCR detection primer set for waterfowl parvovirus, duck enteritis virus and goose astrovirus, the multiplex PCR detection primer set includes the following three groups:

[0054] (1) A primer set for detecting waterfowl parvovirus, a primer set consisting of GPV-MDPV-F and GPV-MDPV-R, wherein:

[0055] The nucleotide sequence of GPV-MDPV-F is:

[0056] 5'-TGGAAGAAGGCAGTGAGT-3';

[0057] The nucleotide sequence of GPV-MDPV-R is:

[0058] 5'-AGGTCCGTAGAGCCATAT-3';

[0059] The detection target gene is Rep1 gene, and the primer size is 746bp;

[0060] (2) primer set for detecting duck enteritis virus, a primer set consisting of DEV-F and DEV-R, wherein:

[0061] The nucleotide sequence of DEV-F is:

[0062] 5'-CCAAGATACCAGTGATGC-3';

[0063] The nucleotide se...

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Abstract

The invention relates to the technical field of biological detection, and particularly discloses a multiplex PCR (Polymerase Chain Reaction) detection primer group, a kit and a detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus, the multiplex PCR detection primer group, the kit and the detection method disclosed by the invention can be used for simultaneously detecting four viruses, namely GPV, MDPV, DEV and GAstV by completing multiplex PCR amplification in a PCR tube at one time, so that the detection sensitivity is high, and the detection time is short. The detection on the duck tembusu virus, the muscovy duck reovirus, the duck III type hepatitis virus and the duck circovirus is negative, the detection sensitivity is high, the specificity is strong, the repeatability is good, and the detection efficiency of the four viruses is greatly improved; meanwhile, the multiplex PCR reaction system is used for detection, so that PCR and electrophoresis reagents and experimental consumables can be effectively saved, and the electrophoresis detection time and cost are reduced.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a multiplex PCR detection primer set, kit and detection method for waterfowl parvovirus, duck enteritis virus and goose astrovirus. Background technique [0002] Waterfowl parvoviruses mainly include Goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV), both of which belong to the family Parvoviridae and the genus Parvovirus, and are currently a smaller class of animal viruses. Stranded DNA virus. MDPV can only infect Muscovy ducks, while GPV hosts widely include geese and Muscovy ducks, and the new GPV can also cause short beak dwarfism syndrome in ducks. Duck enteritis virus (DEV) belongs to the Herpesviridae family and belongs to the genus Marekvirus. Its nucleic acid is linear and double-stranded DNA. It can infect waterfowl such as ducks and geese. Goose astrovirus (Gooseastrovirus, GAstV) belongs to the Astroviridae family and belongs to the genus Astrov...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11
Inventor 戴银李梅珍殷冬冬尹磊王洁茹胡晓苗赵瑞宏沈学怀潘孝成周学利侯宏艳张朝霞
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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