Preparation method and use method of injectable bionic antioxidant hydrogel

A hydrogel and antioxidant technology, applied in medical science, bandages, etc., can solve the problems of limited normal activities, re-infection, and inability to keep the wound moist, so as to solve the problem of insufficient contact time and reduce the pain of patients.

Pending Publication Date: 2022-04-12
GUANGXI XINYE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the coverage of the wound dressing is not easy to fix. When using it, it is necessary to pay attention to waterproofing, and the wound dressing needs to be changed, etc., or the fixation of the wound restricts normal activities and cannot keep the wound moist. There are many application limitations, which often restrict the normal activities of the injured. with the risk of reinfection
Moreover, wound dressing treatment mainly focuses on the removal of extracellular active oxygen in skin tissue, but cannot effectively remove active oxygen in skin tissue cells.

Method used

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  • Preparation method and use method of injectable bionic antioxidant hydrogel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Step (1) Cross-linking synthesis of complex proteins: Take 0.4g mussel mucin, 0.2g silk fibroin, 0.07g sodium hyaluronate, 0.15g acetylcysteine, add to 100ml water and stir evenly to obtain a homogeneous solution , and then use the rotary evaporation method to obtain the concentrated solution from the homogeneous solution, then place the concentrated solution in a dialysis bag, place the dialysis bag in sterilized distilled water for dialysis, and freeze-dry the dialysis to obtain a composite protein powder;

[0023] Step (2) Preparation of VE and VC guest inclusion complexes: Dissolve 0.005g of the complex protein powder in step (1) in 100mL sterile water under aseptic conditions and stir to obtain a complex protein solution; then 10ml volume concentration of 0.1% VE-ethanol solution was dropped dropwise into the complex protein solution, placed on a shaking table at 40°C for 28 hours, and then the solution was distilled under reduced pressure at 40°C to obtain 50ml of ...

Embodiment 2

[0032] A preparation method for injectable biomimetic antioxidant hydrogel, which comprises the following steps:

[0033] Step (1) Cross-linking synthesis of complex proteins: Take 0.5g mussel mucin, 0.25g silk fibroin, 0.08g sodium hyaluronate, 0.16g acetylcysteine, add to 100ml water and stir evenly to obtain a homogeneous solution , and then use the rotary evaporation method to obtain the concentrated solution from the homogeneous solution, then place the concentrated solution in a dialysis bag, place the dialysis bag in sterilized distilled water for dialysis, and freeze-dry the dialysis to obtain a composite protein powder;

[0034] Step (2) Preparation of VE and VC guest inclusion complexes: Dissolve 0.01g of the complex protein powder in step (1) in 100mL of sterile water under sterile conditions and stir to obtain a complex protein solution; then 10ml volume concentration of 0.1% VE-ethanol solution was dropped dropwise into the complex protein solution, placed on a sh...

Embodiment 3

[0043] Step (1) Cross-linking synthesis of complex proteins: Take 0.6g mussel mucin, 0.3g silk fibroin, 0.09g sodium hyaluronate, 0.17g acetylcysteine, add to 100ml water and stir evenly to obtain a homogeneous solution , and then use the rotary evaporation method to obtain the concentrated solution from the homogeneous solution, then place the concentrated solution in a dialysis bag, place the dialysis bag in sterilized distilled water for dialysis, and freeze-dry the dialysis to obtain a composite protein powder;

[0044] Step (2) Preparation of VE and VC guest inclusion complexes: Dissolve 0.015g of the complex protein powder in step (1) in 100mL sterile water under aseptic conditions and stir to obtain a complex protein solution; then 10ml volume concentration of 0.1% VE-ethanol solution was dropped dropwise into the composite protein solution, placed on a shaking table at 50°C for 20 hours, and then the solution was distilled under reduced pressure at 50°C to obtain 50ml o...

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Abstract

The invention relates to the technical field of hydrogel, in particular to a preparation method and a use method of injectable bionic anti-oxidation hydrogel, and the injectable bionic anti-oxidation hydrogel is constructed through crosslinking synthesis of compound protein, preparation of VE and VC guest inclusion complexes and preparation of the anti-oxidation hydrogel. The wound dressing can simulate a cell antioxidant defense system to construct the wound dressing, eliminate the overexpression of reactive oxygen species (ROS) in wound tissues, reduce the damage of the overexpression ROS to cells, tissues and organs, not only can eliminate extracellular reactive oxygen of skin tissues, but also can effectively eliminate reactive oxygen in cells of the skin tissues; the tissue adhesion and the self-healing capability of the wound dressing help the wound dressing to resist mechanical deformation caused by limb movement, and the problems that the dressing is insufficient in contact time and low in durability are effectively solved.

Description

technical field [0001] The invention relates to the technical field of hydrogels, in particular to a method for preparing and using an injectable biomimetic anti-oxidation hydrogel. Background technique [0002] Oxidative stress injury is one of the important mechanisms of multiple organ dysfunction in early burn patients. In the early stage of severe burns, a large amount of body fluid is lost. A large amount of reactive oxygen species (ROS) produced in the early stage of burn can damage the lipid, protein and nucleic acid components of tissues and cells, and cooperate with inflammatory factors and vasoactive substances to cause changes in cell structure and function, resulting in severe oxidation. damage. [0003] At present, traditional wound treatment and repair methods are mainly covered by wound dressings. For example, patent CN111265710A discloses a preparation method of a new type of bionic skin wound dressing based on MAP / PRF composite. Biomimetic synthesis of MA...

Claims

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Application Information

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IPC IPC(8): A61L26/00
Inventor 苏雪梁文棋梁奕福梁超余曾金萍方祖丽李艺华辜衍丽余勇华
Owner GUANGXI XINYE BIOLOGICAL TECH
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