Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Functional banana polysaccharide BPF2 as well as preparation and application thereof

A BPF2, functional technology, applied in allergic diseases, antitoxins, drug combinations, etc., can solve the problems of large molecular weight range of banana polysaccharide, high toxicity of organic reagents, and complicated procedures, so as to alleviate retinopathy and prevent retinopathy. , the effect of reducing mRNA expression

Active Publication Date: 2022-03-25
ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The separation and purification method reported in the above literature or the obtained banana polysaccharide has the following problems: the separation and purification of banana polysaccharide by passing through the column is relatively cumbersome; the obtained banana polysaccharide has a large molecular weight range and is not uniform; Sevag reagent is used to remove protein impurities in the polysaccharide (Sevag method), the organic reagents used are highly toxic and complicated to operate, and are not suitable for the production of food, medicine and health products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Functional banana polysaccharide BPF2 as well as preparation and application thereof
  • Functional banana polysaccharide BPF2 as well as preparation and application thereof
  • Functional banana polysaccharide BPF2 as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: A kind of preparation method of functional banana polysaccharide BPF2

[0046] Specifically include the following steps:

[0047] S1, extract: the banana pulp is beaten, use volume concentration≤15% ethanol aqueous solution in the hot water bath of 80-95 ℃ (the preferred 90 ℃ of present embodiment) with solid-liquid ratio (the quality of the banana pulp obtained by the banana pulp beating and ethanol) The volume ratio of the aqueous solution) is 1:(10-30) stirring and leaching for 2 hours, filtering to remove residue and recovering the filtrate. The above extraction process was repeated twice, and the extraction solution was mixed with the filtrate. Centrifuge at 8000rpm / min for 10min to remove excess impurities and protein, and concentrate the extract and filtrate in a rotary evaporator at a temperature of 60°C and a rotation speed of 140r / min.

[0048] During the preferred extraction of the present embodiment, the solid-liquid ratio of the banana pulp ...

Embodiment 2-5

[0059] Compared with embodiment 1, the main difference of embodiment 2-5 preparing banana polysaccharide BPF2 is as shown in table 1 below:

[0060] Table 1 embodiment 1-5 prepares the step difference of banana polysaccharide BPF2

[0061]

[0062] Characterization Experiment 1: Monosaccharide Composition Analysis

[0063] GC chromatographic conditions: the chromatographic system used was Thermo ICS5000+ ion chromatography system (ICS500+, ThermoFisher Scientific, USA), and Dionex TM CarboPac TM PA20 liquid chromatography column, the injection volume is 20μL. Mobile phase A (H 2 O), mobile phase B (100mM NaOH), the column temperature is 30 ° C, and the monosaccharide composition is analyzed using an electrochemical detector.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of food, medicine and chemical industry, and particularly discloses a preparation method of functional banana polysaccharide BPF2, which comprises the following steps: S1, pulping banana pulp, and extracting by using an ethanol aqueous solution with the volume concentration of less than or equal to 15%; s2, precipitating polysaccharide by adopting ethanol with the volume concentration of more than or equal to 95%; and S3, purifying the polysaccharide obtained in S2 to obtain the banana polysaccharide BPF2. The invention also discloses the banana polysaccharide BPF2 obtained by the preparation method, and an application of the banana polysaccharide BPF2 in preparation of medicines or foods. The banana polysaccharide BPF2 provided by the invention has remarkable scavenging capacity and total antioxidant capacity on hydroxyl free radicals and superoxide anion free radicals; the proliferation activity and the phagocytic activity of RAW264.7 cells can be obviously activated, the secretion of anti-inflammatory cytokines can be induced and promoted, and the inflammatory response can be inhibited; the retinopathy can be relieved by inhibiting migration of human retinal pigment epithelial cells ARPE-19 and reducing expression of VEGF (vascular endothelial growth factor) and ICAM-1 of the ARPE-19 cells.

Description

technical field [0001] The invention belongs to the technical fields of food, medicine and chemical industry, and in particular relates to a functional banana polysaccharide BPF2 and its preparation and application. Background technique [0002] Banana belongs to the Musa family (Musaeae) and is a herbaceous fruit tree. my country is rich in banana resources, with high edible and medicinal value. At present, the research and development of bananas is mainly concentrated on the production and rough processing of bananas, such as banana drinks, banana wine and dried bananas, etc. There are still few researches on the activity of bananas. . [0003] Banana polysaccharide is a soluble polysaccharide substance with a high content in ripe bananas. It has been reported in the literature that it has a certain immune regulation effect, can promote the proliferation of intestinal beneficial bacteria, and has the ability to regulate the health of the gastrointestinal tract and alleviat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P39/06A61P39/00A61P37/00A61P27/02A23L33/125
CPCC08B37/0003C08B37/006A61P39/06A61P39/00A61P37/00A61P27/02A23L33/125Y02A50/30
Inventor 戎瑜吕岱竹王明月宋佳
Owner ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com