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Artificial nerve sheath hydrogel repair system as well as preparation method and application thereof

A technology for repairing systems and artificial nerves, which can be applied to nervous system diseases, medical formulas, and medical preparations containing active ingredients, etc. It can solve problems such as incompatibility of internal structures, affecting nerve regeneration and functional recovery, slippage, etc., and achieve good results. Tissue repair effect, good biocompatibility, and simple preparation method

Active Publication Date: 2022-03-25
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this treatment has significant deficiencies such as surgical injury, nerve tension inhibiting axonal regeneration, and neoplastic pain remain challenging.
In microsurgical operations such as neuroepithelial suture commonly used in clinical practice, once there are problems such as internal structure incompatibility, gaps, twists, overlaps, and slippages between the sutured nerve stumps, the regenerated axons will not be able to grow smoothly. Schwann duct, thereby affecting nerve regeneration and functional recovery

Method used

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  • Artificial nerve sheath hydrogel repair system as well as preparation method and application thereof
  • Artificial nerve sheath hydrogel repair system as well as preparation method and application thereof
  • Artificial nerve sheath hydrogel repair system as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The specific composition and structure of the artificial nerve sheath are as follows: figure 1 shown. The preparation method comprises the following steps:

[0040] (1) Preparation of methacrylic anhydride-modified gelatin: Weigh 2 g of gelatin and add it to 50 mL of normal saline and heat the solution to 40-50 ° C, then continue stirring for 0.5-1 h to melt the gelatin, then add 500 μL of formazan Base acrylic anhydride solution (0.1g / mL), react for 4-5h under the condition of avoiding light, put the reacted solution into a dialysis bag and dialyze for 3-4 days, freeze and dry the dialyzed solution to obtain methacrylic acid Anhydride-modified gelatin. Adopt the test result of total reflection infrared spectrum and nuclear magnetic resonance spectrum to show: successfully prepared methacrylated gelatin ( figure 2 and Figure 3a-3b ), the specific test results are as follows:

[0041] ATR-FTIR and NMR Spectroscopy Test Results

[0042] The methacrylated gelatin t...

Embodiment 2

[0057] A preparation method of an artificial nerve sheath hydrogel repair system, comprising the following steps:

[0058] (1) Methacrylic anhydride-modified gelatin: add 0.5 g of gelatin to 10 mL of normal saline and heat the solution to 40 ° C. Under continuous stirring (stirring speed is 300 r / min), melt the gelatin and add methacrylic anhydride 50 μL of the solution was reacted for 4 hours, dialyzed for 3 days, and freeze-dried to obtain methacrylic anhydride-modified gelatin.

[0059] (2) Dopa-grafted acrylic acid: 0.4 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was first added to dilute acrylic acid solution (7.5 mL of acrylic acid The volume ratio of the solution to the pure is 1:1.1, and the acrylic acid solution is commercially available (chemically pure) to react for 0.5h, then add 0.2g of N-hydroxysuccinimide (NHS) to react for 3h, and finally add dopamine hydrochloride to react After 3 days, dialyze, freeze, and dry to obtain dopa-grafte...

Embodiment 3

[0064] A preparation method of an artificial nerve sheath hydrogel repair system, comprising the following steps:

[0065] (1) Methacrylic anhydride-modified gelatin: Add 2 g of gelatin to 50 mL of normal saline and heat the solution to 50 ° C. Under continuous stirring (stirring speed is 400 r / min), melt the gelatin and add methacrylic anhydride solution 500 μL was reacted for 5 hours, dialyzed for 4 days, and freeze-dried to obtain methacrylic anhydride-modified gelatin.

[0066] (2) Dopa-grafted acrylic acid: 1.5 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was first added to dilute acrylic acid solution (30 mL, in which acrylic acid The volume ratio of the solution to the pure is 1.5:1.2, and the acrylic acid solution is commercially available (chemically pure) to react for 1 h, then add 1.2 g of N-hydroxysuccinimide (NHS) to react for 4 h, and finally add dopamine hydrochloride to react for 4 h Day, dialyzed, frozen, and dried to obtain dopa-gra...

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Abstract

The invention relates to an artificial nerve sheath hydrogel repairing system as well as a preparation method and application thereof. The artificial nerve sheath hydrogel repairing system is prepared from the following raw materials in parts by weight: 2 to 3 parts of gelatin, 0.5 to 1 part of methacrylic anhydride modified gelatin, 30 to 35 parts of acrylic acid, 1 to 1.5 parts of dopamine grafted acrylic acid, 6 to 8 parts of tannic acid and 1 to 1.6 parts of methacryloyloxyethyl trimethyl ammonium chloride. The bionic artificial nerve sheath hydrogel repair system is prepared through 3D polymerization under the condition of thermal initiation by adjusting the content of each component. Compared with the prior art, the prepared artificial nerve sheath hydrogel repair system has good stability and excellent biocompatibility, has good repair and regeneration effects on cut sciatic nerves of rats, provides a new method for development of a repair system of damaged nervous tissues, and has wide application prospects. Wide application prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of gel production, in particular to an artificial nerve sheath hydrogel repair system and its preparation method and application. Background technique [0002] Peripheral nerves play an important physiological role in the transmission of information between the brain and various parts of the body. But when nerves are damaged, it often results in chronic pain, paralysis of limbs and even severe disability. Unlike the central nervous system, peripheral nerves have a limited ability to regenerate after injury. Currently, the general strategy for repairing peripheral nerve injuries consists primarily of end-to-end suturing of nerves under a microscope. However, this treatment has significant deficiencies, such as surgical injury, nerve tension inhibiting axon regeneration, and tumor-induced pain remain challenging. In microsurgical operations such as neuroepithelial suture commonly used in clinical practice, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/52A61L27/50A61L27/54A61K38/39A61K9/06A61P25/02C08F289/00C08F220/06C08F220/58C08F220/34
CPCA61L27/222A61L27/52A61L27/50A61L27/54A61K38/39A61K9/06A61P25/02C08F289/00A61L2430/32A61L2300/412C08F220/06C08F220/58C08F220/34
Inventor 陈玉洁杨秩蔡超朱慧敏李华刘河洲
Owner SHANGHAI JIAO TONG UNIV
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