Triploid oyster ploidy identification and genetic material source analysis method

Abstract

The invention relates to a triploid oyster ploidy identification and genetic material source analysis method, and belongs to the field of molecular cell genetics, and the method comprises the following steps: 1) preparing a metaphase chromosome specimen of a single individual triploid oyster; 2) performing mode analysis on somatic cell chromosomes; 3) carrying out multiple specific PCR (Polymerase Chain Reaction); 4) analyzing genetic material sources of triploid oysters; the preparation steps of the oyster somatic cell chromosome are simplified, the analysis time is shortened, and the whole process only needs 2-3 hours. The prepared chromosome slice is clean and clear; through molecular identification of multiple specific PCR, a tetraploid male parent and a diploid female parent of triploid oysters can be further determined, analysis of genetic material composition, chromosome ploidy, parent oyster identification and the like is carried out on the triploid oysters on chromosome and molecular levels, chromosome preparation and analysis can be combined with molecular identification of multiple specific PCR for use, and the method is simple and convenient to operate. The method is helpful for identifying the source of the triploid parent, so that the quality of the triploid offspring seeds

Description

technical field [0001] The invention belongs to the field of molecular cytogenetics, and in particular relates to a method for identifying triploid oyster ploidy and analyzing the source of genetic material. Background technique [0002] Chromosome is the carrier of genetic material. Studying the number and composition of chromosomes of a species can not only identify the ploidy of the species, but also help determine the breeding method of the species. The molecular identification of genetic material can further determine the parental and maternal characteristics of the offspring. Genetic information has important guiding significance for the development and utilization of resources and genetic breeding. Oysters are generally rich in active elements such as glycogen, amino acids, and taurine, and are a natural sea treasure with food tonic effects. Compared with diploid oysters, triploid oysters grow faster, have larger individuals, and have higher quality. Triploid long o...

AI Technical Summary

Problems solved by technology

There are two main methods of production of triploid oysters: traditionally during artificial insemination, fertilized eggs are treated with different methods (such as the chemical reagents cytochalasin B and 6-dimethylaminopurine) to inhibit second polar body discharge To produce triploid oysters, the drugs used to artificially induce triploid oysters are toxic, the procedures are complicated, the induction rate is low and unstable, and it is impossible to carry out large-scale production; Crossbreed with diploid oyster female parent to produce 100% triploid, no need for drugs and any treatment

Microfluorescence and flow cytometry both require highly specialized operations and expensive equipment, and neither can identify non-integral cells with one or more chromosomes or one or more chromosomes missing in somatic cells. ploidy, neither of these tests can rule out the risk of genetic defects, so neither can accurately identify the quality of oysters

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