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Identification method of three medicinal materials derived from Chinese honey locust and application of identification method

A technology of acacia and traditional Chinese medicine, applied in the field of identification of traditional Chinese medicine, can solve the problems of unclear interpretation of different components, and achieve the effect of convenient detection method and high repeatability

Active Publication Date: 2022-03-15
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the above three medicinal materials all come from the same plant of saponins, the types of ingredients contained are similar, such as triterpenoid saponins, alkaloids, sterols, coumarins, flavonoids, lignans, phenols, etc., and currently The explanation for the difference in composition between the three traditional Chinese medicines is not clear

Method used

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  • Identification method of three medicinal materials derived from Chinese honey locust and application of identification method
  • Identification method of three medicinal materials derived from Chinese honey locust and application of identification method
  • Identification method of three medicinal materials derived from Chinese honey locust and application of identification method

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Experimental program
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Effect test

Embodiment 1

[0078] The embodiment of the present invention provides a process for obtaining differential identification markers.

[0079] 1. Sample preparation

[0080] The 45 batches of large saponins, pig tooth soap and saponins shown in Table 1 were pulverized respectively, and passed through a No. 4 sieve. Accurately weigh 200mg of powder and extract with 8mL of 50% v / v methanol, ultrasonic at 40°C for 1h, let stand to cool down to make up for weight loss, centrifuge at 14000rmp for 10min, take the supernatants into 10mL volumetric flasks, and make up to the mark After mixing, pipette 1mL into 1.5mL EP tubes with a pipette gun, centrifuge at 14000rmp for 10min, and take the supernatant. Draw the same volume of the supernatant prepared by Dazao, Zhuyazao, and Zaojiao, and mix to obtain the QC samples of three different medicinal materials: Dazao, Zhuyazao, and Zaojiao. Used to monitor instrument stability during sequence runs.

[0081] 2. Chromatographic conditions

[0082] Station...

Embodiment 2

[0129] The embodiment of the present invention provides a method for identifying big saponins, pig tooth soap and saponins thorns.

[0130] S1. Processing of samples to be tested

[0131] Weigh a certain amount of medicinal materials, crush them, and pass through a No. 4 sieve. Accurately weigh 200mg of powder and extract with 8mL of 50% v / v methanol, ultrasonic at 40°C for 1h, let stand to cool down to make up for weight loss, centrifuge at 14000rmp for 10min, take the supernatants into 10mL volumetric flasks, and make up to the mark After mixing, pipette 1 mL into 1.5 mL EP tubes with a pipette gun, centrifuge at 14000 rpm for 10 min, and take the supernatant to obtain the sample solution to be tested.

[0132] S2. Detection

[0133] The sample solution to be tested is detected by ultra-high performance liquid chromatography / quadrupole time-of-flight mass spectrometry (UHPLC / Q-TOF-MS) and obtains a base peak figure (BPI figure);

[0134] The ultra-high performance liquid ...

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Abstract

The invention relates to the technical field of traditional Chinese medicine identification, in particular to an identification method of three traditional Chinese medicines derived from Chinese honey locust and application thereof. According to the method, a reversed-phase ultra-high performance liquid chromatography and quadrupole time-of-flight mass spectrometry combined technology is adopted, so that three markers (Saikachinoside A, Locust A and Locust B) of the Chinese honeylocust fruit and the fructus gleditsiae and four markers (2-Methyl-7-(2-Methyl-2-Propanyl)-2-[(3E, 7E)-4, 8, 12-trimetyl-3, 7, 8-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7-trimetyl-3, 7- According to the present invention, with the application of the method, the accurate analysis and identification of the three traditional Chinese medicines such as the Chinese honeylocust fruit, the fructus gleditsiae and the spina gleditsiae can be achieved, wherein the three traditional Chinese medicines comprise the Chinese honeylocust fruit, the fructus gleditsiae and the spina gleditsiae, such as the Chinese honeylocust fruit, the fructus gleditsiae, the oleanolic acid isomer (C30H46O2), the taxifolin isomer (C30H48O3), the taxifolin isomer (C30H48O2) and the betulin isomer (C30H48O2).

Description

technical field [0001] The present invention relates to the technical field of identification of Chinese medicines, in particular to a method for identifying three kinds of Chinese medicines derived from Acacia saponaria and its application. Background technique [0002] Honey locust, also known as saponins, is a natural raw material for pharmaceutical products, health care products, cosmetics and cleaning products, and has high economic value. Three kinds of medicinal materials derived from saponins are included in the Chinese Pharmacopoeia, including: Dazaoba, Zhuyazao and Zaojiao. Saponaria saponin is the dry ripe fruit of the leguminous plant Acacia saponica; it can resuscitate, eliminate phlegm, unblock the orifices, etc. It is mainly used for the treatment of sudden coma, inability to open the mouth, bronchial asthma, constipation, etc. Pig tooth soap is the deformed small pod of acacia; it has the effects of opening the orifice, clearing phlegm, searching wind, and k...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/72
CPCG01N30/88G01N30/72G01N2030/884G01N2030/027Y02A50/30
Inventor 杨文志李雪胡莹谢胡敏王洪达荆绮王慧敏徐晓艳
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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