Biochemical composite material as well as preparation method and application thereof

A composite material and biochemical technology, applied in the cross field of microbiology and environmental engineering technology, can solve expensive problems, achieve low cost, promote calcium deposition, and save production costs

Pending Publication Date: 2022-03-01
GUANGDONG TECHNION ISRAEL INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, biochar is more expensive as a pyrolytic substrate than naturally available fibrous or porous substances

Method used

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  • Biochemical composite material as well as preparation method and application thereof
  • Biochemical composite material as well as preparation method and application thereof
  • Biochemical composite material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1, enrichment, purification and urease activity analysis of urease-producing strains

[0051] 1. Enrichment of urease-producing strains

[0052] 1.1 The urease-producing strain of the present invention is isolated from soil collected from an electronic waste site in Guiyu Town, Guangdong Province, and cultured under static culture conditions of 25°C to 35°C for 24 hours. After removing large particles of impurities through a 2mm aperture sieve, take 5 grams of each portion and place them in a clean 50mL conical flask, add 25mL, 85% sodium chloride (NaCl) solution, and prepare 50mg / L of chlorine for the solution Cadmium (CdCl 2 ) and 4% urea;

[0053] 1.2 Put the solution prepared in step 1.1 into a constant temperature shaking incubator. The set temperature of the constant temperature shaking incubator is 37° C., the set speed is 120 rpm, and the incubation time is 7 days.

[0054] 2. Purification of urease-producing strains

[0055] 2.1 Take the solution tr...

Embodiment 2

[0090] Embodiment 2, a kind of biochemical composite material and preparation method thereof

[0091] 1) According to the urease activity and bacterial optical density data obtained in step 3.8, reasonably select Bacillus sp.WA with high urease activity and high optical density as one of the raw materials for biochemical composite materials, and inoculate them into a petri dish based on nutrient agar , stored in a refrigerator at 4°C;

[0092] 2) Prepare urea nutrient solution (NBU) as the formation medium of biochemical composite materials, the specific method is:

[0093] Weigh 2.6 grams of nutrient broth powder and dissolve it in 170 mL of deionized water;

[0094] Continuing from the previous step, add 20 mL of 250 mM pinhole sterilized calcium chloride (CaCl 2 ) solution;

[0095] Continuing from the previous step, add 10mL of 40% pinhole sterilized urea solution;

[0096] Continuing from the previous step, add 60 μL of pinhole sterilized sodium hydroxide (NaOH) solut...

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PUM

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Abstract

The invention relates to the technical crossing field of microorganisms and environmental engineering, and particularly discloses a biochemical composite material as well as a preparation method and application thereof. Wherein the biochemical composite material comprises a urease-producing strain and a sustainable material carrier, the urease-producing strain is preserved in Guangdong Microbial Culture Collection Center on March 15, 2021, and the preservation number is GDMCC NO: 61559. According to the invention, urease-producing strains capable of promoting microorganism-induced calcium carbonate deposition are screened out; the biochemical composite material disclosed by the invention has the advantages of high urease production capacity, optimal bacterial reproduction capacity and high resistance to a heavy metal environment, and is beneficial to repairing heavy metal polluted soil; the corncob powder is adopted as a continuous material carrier, the cost is low, and the corncob powder serving as the carrier has an extremely high promoting effect on enrichment of urease-producing strains in soil and calcium deposition induced by the strains.

Description

technical field [0001] The invention relates to the interdisciplinary field of microorganisms and environmental engineering technologies, in particular to a biochemical composite material and its preparation method and application. Background technique [0002] Heavy metal soil pollution poses a threat to biodiversity, agricultural production, and the overall environment and human health. Many countries currently employ various soil remediation techniques to reduce soil metal concentrations, such as physicochemical remediation and phytoremediation, however, these techniques still have many shortcomings. Microbial calcification, as a relatively new research field in environmental rock and soil in recent years, is a special biomineralization method. Its core technology is MICP (Microbially Induced Calcite Precipitation, microbially induced calcium carbonate precipitation). Through certain types of microorganisms in nature, its Metabolism can produce urease that decomposes ure...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/80C12N11/02B09C1/10C12R1/07
CPCC12N9/80C12Y305/01005C12N11/02C12N1/20B09C1/10
Inventor 安澈李维拉杨逸凡
Owner GUANGDONG TECHNION ISRAEL INST OF TECH
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