Cordyceps militaris neutral oligosaccharide for regulating lung lymphocyte differentiation as well as preparation method and application of cordyceps militaris neutral oligosaccharide

A technology of lymphocytes and neutral oligosaccharides, which is applied to the preparation of sugar derivatives, chemical instruments and methods, and medical preparations containing active ingredients to achieve the effects of regulating differentiation levels, reducing lung index, and increasing levels

Pending Publication Date: 2022-03-01
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the role of Cordyceps militaris oligosaccharides in regulating the differentiation of lung lymphocytes.

Method used

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  • Cordyceps militaris neutral oligosaccharide for regulating lung lymphocyte differentiation as well as preparation method and application of cordyceps militaris neutral oligosaccharide
  • Cordyceps militaris neutral oligosaccharide for regulating lung lymphocyte differentiation as well as preparation method and application of cordyceps militaris neutral oligosaccharide
  • Cordyceps militaris neutral oligosaccharide for regulating lung lymphocyte differentiation as well as preparation method and application of cordyceps militaris neutral oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation and structural analysis of polysaccharide from Cordyceps militaris

[0045] 1.1 Cordyceps militaris polysaccharide extraction

[0046]Prepare 200g of dried Cordyceps militaris, crush them, add 80vol.% ethanol solution at a solid-liquid ratio of 1:20 (g:mL), ultrasonicate for 20min at 500W and 55°C, and pour out the supernatant after standing still for 2h, and pour out the remaining precipitate according to the material Add 80vol.% ethanol solution at a liquid ratio of 1:10 (g:mL), ultrasonicate at 500W and 55°C for 20min, and pour out the supernatant after standing still for 2h; collect the remaining precipitate, heat and dry at 50°C to obtain Cordyceps militaris powder.

[0047] Weigh the Cordyceps militaris powder, add 30 times the volume of hot water at 80°C and pour it into the wall breaker, boil the wall and extract it for 8 minutes, centrifuge the supernatant, add three times the volume of absolute ethanol and let it rest for 12 hours; collec...

Embodiment 2

[0058] Example 2 Study on the mechanism of Cordyceps militaris polysaccharide improving lymphocyte differentiation activity in asthmatic mice

[0059] 2.1 Animal experiment design and treatment

[0060] 2.1.1 Animal grouping

[0061] Ninety Balb / c mice were randomly divided into 6 groups, namely blank control group (Control), model group (OVA), dexamethasone group (OVA+DEX), high-dose neutral oligosaccharide group of Cordyceps militaris (OVA) +CMP 200), Cordyceps militaris neutral oligosaccharide medium dose group (OVA+CMP 100), Cordyceps militaris neutral oligosaccharide low dose group (OVA+CMP 50), 15 rats in each group.

[0062] 2.1.2 Animal model establishment and administration

[0063] The mice in each group except the blank group were intraperitoneally injected with 0.2ml of sensitizing solution on the 1st, 8th, and 15th days after the establishment of the model, and the mice in the blank control group were injected with an equal volume of normal saline intraperitonea...

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Abstract

The invention discloses cordyceps militaris neutral oligosaccharide for regulating lung lymphocyte differentiation and a preparation method and application thereof, and belongs to the field of natural product development and utilization, the cordyceps militaris neutral oligosaccharide comprises glucose, mannose, galactose, xylose, arabinose, rhamnose, ribose and fucose according to the mass ratio of 269.45: 72.01: 38.47: 9.99: 6.27: 1.42: 1.19: 1; the cordyceps militaris neutral oligosaccharide can reduce the lung index of a mouse with allergic asthma, reduce the serum IgE level, increase the INF-gamma content, inhibit expression of IL-4, IL-5, IL-13 and IL-17A inflammatory factors, promote Nrf2 signal channel transduction and inhibit NF-kappa B signal channel transduction, so that the differentiation level of mouse lymphocyte Th1 / Th2 is adjusted, and the lung inflammation symptom is relieved.

Description

technical field [0001] The invention relates to the field of development and utilization of natural products, in particular to a neutral oligosaccharide from Cordyceps militaris for regulating the differentiation of lymphocytes in the lungs and its preparation method and application. Background technique [0002] Pulmonary lymphocyte differentiation can lead to a variety of lung diseases, such as allergic asthma, chronic obstructive pulmonary disease, pneumonia, etc., are all caused by lung inflammation, and the degree of lung inflammation is determined by the differentiation of pulmonary lymphocytes. Allergic asthma, a pulmonary inflammatory disease alone, has as many as 300 million patients worldwide, and it is increasing rapidly at a rate of 20% to 25% every 10 years. Therefore, pulmonary inflammatory diseases not only cause patients with dyspnea, loss of appetite, weight loss, depression, anxiety, but also bring a heavy burden to governments and health departments of var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7004A61P29/00C07H1/08C07H3/02A23L33/125
CPCA61K31/7004C07H3/02C07H1/08A61P29/00A23L33/125A23V2002/00A61K2300/00A23V2200/30A23V2250/60A23V2250/602A23V2250/608A23V2250/61A23V2250/618A23V2250/624A23V2250/626A23V2300/14A23V2300/38C08L5/00C08B37/006C08B37/0003A61K31/702A61K36/068
Inventor 苏玲宋蓝月刘淑艳王迪刘洋戴晓婧
Owner JILIN AGRICULTURAL UNIV
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