Magnetic molecularly imprinted electrochemical sensor and preparation method and application thereof

A magnetic molecular imprinting and electrochemical technology, applied in the direction of material electrochemical variables, etc., can solve the problems of time-consuming, weak selectivity, and long sample detection time, and achieve good anti-interference characteristics and good specific recognition.

Pending Publication Date: 2022-01-25
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the methods commonly used to detect the content of methotrexate in samples are high-performance liquid chromatography, capillary electrophoresis, and electrochemical detection methods, while the detection of high-performance liquid chromatography usually requires more complicated pretreatment of the samples to be tested, which consumes a lot of time. More time, resulting in a longer sample detection time; although capillary electrophoresis saves samples, the reproducibility is limited due to the small amount of sample injection; the electrochemical detection of existing electrochemical sensors is mainly based on conventional electrochemical modification Materials such as acetylene black and graphene, etc., the selectivity of the electrode to methotrexate is relatively weak, that is, the ability to resist the interference of some substances that exist at the same time as the detection substance is poor, resulting in poor practical application of electrochemical detection

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  • Magnetic molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Magnetic molecularly imprinted electrochemical sensor and preparation method and application thereof
  • Magnetic molecularly imprinted electrochemical sensor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for preparing a magnetic molecularly imprinted electrochemical sensor, comprising the following steps,

[0046] S1: Fully grind 280mg of carbon powder, 30 μL of paraffin oil, and 70 mg of methotrexate magnetic molecularly imprinted polymer in a quartz mortar for 10 to 30 minutes to make a homogeneous magnetic molecularly imprinted carbon paste powder;

[0047] S2: Fill the magnetic molecularly imprinted carbon paste powder prepared in step S1 into the carbon paste electrode as a filler, and polish the electrode surface on a smooth weighing paper to prepare a magnetic molecularly imprinted carbon paste electrode;

[0048] S3: The magnetic molecularly imprinted carbon paste electrode prepared in step S2 was used as a working electrode, the platinum electrode was used as a counter electrode, and the saturated calomel electrode was used as a reference electrode to prepare a magnetic molecularly imprinted electrochemical sensor.

[0049] Further, the method for prep...

Embodiment 2

[0082] The difference between Example 2 and Example 1 is only that the amount of methotrexate magnetic molecularly imprinted polymer added in step S1 is different, and the rest are the same.

[0083] Add 20, 30, 40, 50, 60, 80, and 90 mg of methotrexate magnetic molecularly imprinted polymers respectively, and add the finally prepared magnetic molecularly imprinted carbon paste electrodes with the methotrexate molecularly imprinted polymers in Example 1 The magnetic molecularly imprinted carbon paste electrodes prepared with an amount of 70 mg were compared for performance, and the results were obtained from the attached image 3 shown.

[0084] From attached image 3 It can be seen from the figure that the magnetic molecularly imprinted carbon paste electrode prepared with the addition of 70 mg of methotrexate molecularly imprinted polymer in Example 1 has the largest current, indicating that when the amount of magnetic molecularly imprinted polymer is 70 mg The adsorption ...

Embodiment 3

[0086] The difference between the third embodiment and the first embodiment is only that the amount of paraffin oil added in the step S1 is different, and the rest are the same.

[0087] Add 10, 20, 40, and 50 μL of paraffin oil, respectively, and compare the performance of the finally prepared magnetic molecularly imprinted carbon paste electrode with the magnetic molecularly imprinted carbon paste electrode prepared by adding 30 μL of paraffin oil in Example 1. The results are from the attached Figure 4 shown.

[0088] From attached Figure 4 It can be seen from the figure that the magnetic molecularly imprinted carbon paste electrode prepared with the addition of paraffin oil of 30 μL in Example 1 has the largest current, indicating that the electrode prepared when the amount of paraffin oil is 30 μL has the best adsorption and catalytic effect on methotrexate , this is because when the amount of paraffin oil is less than 30 μL, the graphite powder and the magnetic molecu...

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Abstract

The invention discloses a magnetic molecularly imprinted electrochemical sensor and a preparation method and application thereof. The preparation method of the magnetic molecularly imprinted electrochemical sensor comprises the following steps of: S1, fully grinding carbon powder, paraffin oil and a methotrexate magnetic molecularly imprinted polymer to obtain homogeneous magnetic molecularly imprinted carbon paste powder; S2, filling a carbon paste electrode with the magnetic molecularly imprinted carbon paste powder prepared in the step S1 as a filler, and polishing the surface of the electrode on smooth weighing paper to obtain a magnetic molecularly imprinted carbon paste electrode; and S3, preparing a magnetic molecularly imprinted electrochemical sensor by taking the magnetic molecularly imprinted carbon paste electrode prepared in the step S2 as a working electrode. The electrochemical sensor prepared by utilizing the preparation method disclosed by the invention has better specific recognition on methotrexate, and also has better anti-interference characteristic on structural analogues of methotrexate, such as trimethoprim, metsulfuron-methyl and folic acid.

Description

technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a magnetic molecular imprinted electrochemical sensor and its preparation method and application. Background technique [0002] Methotrexate is a folate reductase inhibitor, orange-yellow crystalline powder, easily soluble in dilute alkali, acid or alkali metal carbonate solution, slightly soluble in dilute hydrochloric acid, almost insoluble in water, ethanol, chloroform , Ether. At present, the methods commonly used to detect the content of methotrexate in samples are high-performance liquid chromatography, capillary electrophoresis, and electrochemical detection methods. More time, resulting in a longer sample detection time; although capillary electrophoresis saves samples, the reproducibility is limited due to the small amount of sample injection; the electrochemical detection of existing electrochemical sensors is mainly based on conventional electrochemica...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 周廷廷孙树凯田清武玄超邓梓雯翟玉娥赵鹏周雨笋
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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