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Method for quantifying effective components of herba ephedrae in lung-heat-clearing and toxin-expelling granules

An effective ingredient, lung-clearing and detoxifying technology, applied in the field of medicine, can solve the problems of inconvenient storage, use and carrying of decoction, and achieve the effects of good applicability and reproducibility, good repeatability and high precision

Pending Publication Date: 2022-01-11
INST OF BASIC RES & CLINICAL MEDICINE CHINA ACAD OF CHINESE MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Qingfei Paidu Granule is a granule improved on the basis of Qingfei Paidu Decoction traditional Chinese medicine compound, which solves the problem of inconvenient storage, use and carrying of the decoction, and is conducive to the popularization and application of the traditional Chinese medicine compound

Method used

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  • Method for quantifying effective components of herba ephedrae in lung-heat-clearing and toxin-expelling granules
  • Method for quantifying effective components of herba ephedrae in lung-heat-clearing and toxin-expelling granules
  • Method for quantifying effective components of herba ephedrae in lung-heat-clearing and toxin-expelling granules

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Experimental program
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Effect test

Embodiment 1

[0057] Measured according to high performance liquid chromatography (Chinese Pharmacopoeia 2020 edition general rule 0512).

[0058] Chromatographic conditions and system suitability test: polar ether-linked phenyl-bonded silica gel is used as filler; acetonitrile is used as mobile phase A, and aqueous solution containing 0.2% triethylamine and 0.2% phosphoric acid is used as mobile phase B, as shown in the following table Gradient elution was carried out according to the provisions in ; the detection wavelength was 210nm; the flow rate was 0.8mL / min; the column temperature was 40°C. The number of theoretical plates calculated based on the ephedrine hydrochloride peak should not be less than 10,000.

[0059]

[0060]

[0061] Preparation of reference substance solution: Take an appropriate amount of ephedrine hydrochloride reference substance and pseudoephedrine hydrochloride reference substance, weigh them accurately, add 50% methanol to make a mixed solution containing...

experiment example 1

[0066] This experimental example is used to illustrate the determination process of the quantitative method of the present invention.

[0067] 1. Instruments and reagents

[0068] Instrument: Japan Shimadzu LC-20AT high performance liquid chromatography, SIL-20A autosampler, CTO-10AS column thermostat, detector: SPD-M20A; KQ-250DE numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd. company), Mettler XS205 analytical balance.

[0069] Chromatographic column: WELCH-UItimate Phenyl-Ether, 4.6*250mm (5μm) Reagents: acetonitrile, methanol, phosphoric acid, chromatographically pure (Fisher Scientific), triethylamine: Sinopharm Chemical Reagent Co., Ltd., chromatographically pure (Fisher Scientific), Watsons high-purity water .

[0070] Reference substances: ephedrine hydrochloride (batch number 171237-201510) and pseudoephedrine hydrochloride (batch number 171241-201809) were purchased from China National Institutes for Food and Drug Control. The purity ...

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Abstract

The invention relates to the technical field of medicines, and particularly discloses a method for quantifying effective components of herba ephedrae in lung-heat-clearing and toxin-expelling granules. According to the method, a high performance liquid chromatography is adopted, and the chromatographic conditions are as follows: polar ether connected phenyl bonded silica gel is taken as a chromatographic column filler, acetonitrile is taken as a mobile phase A, a 0.2% phosphoric acid solution (containing 0.2% triethylamine) is taken as a mobile phase B, and gradient elution is carried out; the gradient elution process is as follows: the volume ratio of the mobile phase A to the mobile phase B is 1: 99 within 0-20 minutes; within 20-20.1 min, the volume ratio of the mobile phase A to the mobile phase B is gradually changed from 1: 99 to 50: 50 at a constant speed; within 20.1-25 min, the volume ratio of the mobile phase A to the mobile phase B is 50: 50; the measured effective components of ephedra are ephedrine hydrochloride and pseudoephedrine hydrochloride. The method provided by the invention is good in repeatability and high in precision, and determination results on different high performance liquid chromatographs and different chromatographic columns have no significant difference, so that the method provided by the invention is good in applicability and reproducibility and suitable for general popularization.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for quantifying the active ingredients of ephedra in Chinese patent medicines. Background technique [0002] Qingfei Paidu Decoction is clinically verified and evolved from classic prescriptions. It is composed of four classic prescriptions: Maxing Shigan Decoction, Xiaochaihu Decoction, Wuling San and Shegan Mahuang Decoction. The prescriptions of the medicine include Xuan, Qing, Spleen, and Stomach. The prescriptions cover a wide range, taking into account cold, heat, dryness, and gastrointestinal problems. After clinical verification, the curative effect is definite and promoted. This prescription can also be used for common people. Cold and flu sufferers. [0003] Qingfei Paidu Granule is a granule improved on the basis of Qingfei Paidu Decoction traditional Chinese medicine compound, which solves the problem of inconvenient storage, use and carrying of the decocti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 司南杨立新周严严王宏洁赵海誉高文雅边宝林马艳刘斌王燕平
Owner INST OF BASIC RES & CLINICAL MEDICINE CHINA ACAD OF CHINESE MEDICAL SCI
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