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New application of composite probiotic preparation to field of oxidation resistance

A compound probiotic and anti-oxidation technology, applied in the field of probiotics, can solve problems such as adverse reactions, decreased immune function, and poor anti-oxidation effect

Pending Publication Date: 2022-01-07
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of anti-oxidation drugs mainly has the following disadvantages: 1) Exogenous supplementation, the body's own functions cannot be truly repaired, long-term use, it is easy to disturb the inherent metabolism, and the affinity is low; 2) The molecular weight is too large, it is difficult to pass through the cell membrane smoothly , if taken orally, it is easily hydrolyzed by protease and loses its activity; 3) There are adverse reactions, such as: nausea, vomiting, headache and rash, and there are also allergies
In addition to drugs, natural antioxidants such as fat-soluble vitamin E, water-soluble vitamin C, and β-carotene can also undergo redox reactions with oxygen free radicals to completely remove oxygen free radicals, but these natural antioxidants It has the following disadvantages: 1) long-term high-dose application of vitamin E can easily cause bleeding, high blood pressure, urticaria, reproductive dysfunction, and decreased immune function; 2) vitamin C can stimulate the stomach and cause hyperacidity; Weaker antioxidant capacity in the body
Therefore, for the damage of excessive active oxygen free radicals in the human body to the damage of human body functions and the initiation of diseases, the current natural antioxidants are not strong enough to resist oxidation, and will also cause side effects such as bleeding, high blood pressure, and hyperacidity. Antioxidant drugs As an exogenous supplement, the molecular weight is too large to be well absorbed by the human body, and the anti-oxidation effect is not good, and it will cause various adverse reactions

Method used

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  • New application of composite probiotic preparation to field of oxidation resistance
  • New application of composite probiotic preparation to field of oxidation resistance
  • New application of composite probiotic preparation to field of oxidation resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of compound probiotic preparation

[0030] The preparation method of compound probiotic preparation comprises:

[0031] a. Add sodium glutamate to the medium in an amount of 20g / L, stir for 10min, heat up to 55°C, and sterilize to obtain a sterilized medium; wherein, the formula of the medium is: skimmed milk powder 80g, whole milk Milk powder 2g, glucose 2g, peptone 10g, yeast extract powder 5g, anhydrous sodium acetate 2g, magnesium sulfate 0.58g and manganese sulfate 0.25g, add water to 1L, adjust the pH value to 6.6;

[0032] b. Inoculate Streptococcus thermophilus S709, Lactobacillus paracasei L578 and Lactobacillus helveticus L551 into the sterilized medium according to the volume ratio of 10% to ferment, and control the fermentation temperature to 42°C and ferment for 30 hours to obtain a fermented liquid, which is sterilized at high temperature, concentrated and dried to obtain a compound probiotic preparation.

Embodiment 2

[0034] Application of compound probiotic preparation in the preparation of antioxidant products

[0035] 1. Materials and equipment

[0036] 1.1, compound probiotic preparation (made in embodiment 1).

[0037] 1.2. Reagents: DPPH free radical detection kit, hydroxyl free radical detection kit, ABTS free radical detection kit, superoxide anion free radical detection kit (all provided by Suzhou Grace Biotechnology Co., Ltd.).

[0038] 2. Test method

[0039] 2.1. Detection of in vitro antioxidant capacity of compound probiotic preparations

[0040] 2.1.1. Sample pretreatment: Dilute the compound probiotic preparation in Example 1 with pure water to 5mg / mL, 10mg / mL, 25mg / mL, 30mg / mL, 50mg / mL, 200mg / mL, 300mg / mL concentrations for in vitro experiments.

[0041] 2.1.2. Determination of DPPH free radical scavenging rate of compound probiotic preparation

[0042] The DPPH free radical detection kit was used to measure the DPPH free radical scavenging ability of the compound prob...

Embodiment 3

[0059] Preparation of compound probiotic preparation

[0060] The preparation method of compound probiotic preparation comprises:

[0061] a. Add sodium glutamate to the medium in an amount of 20g / L, stir for 10min, heat up to 55°C, and sterilize to obtain a sterilized medium; wherein, the formula of the medium is: skimmed milk powder 80g, whole milk Milk powder 2g, glucose 2g, peptone 10g, yeast extract powder 5g, anhydrous sodium acetate 2g, magnesium sulfate 0.58g and manganese sulfate 0.25g, add water to 1L, adjust the pH value to 6.6;

[0062] b. Inoculate Streptococcus thermophilus S709, Lactobacillus paracasei L578 and Lactobacillus helveticus L551 into the sterilized medium according to the volume ratio of 10% to ferment, and control the fermentation temperature to 42°C , fermented for 30 hours to obtain a fermented liquid, and the fermented liquid was concentrated and dried to obtain a compound probiotic preparation.

[0063] Utilize the detection method in embodime...

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Abstract

The invention relates to the technical field of probiotics, and particularly discloses a novel application of a composite probiotic preparation to the field of oxidation resistance. The composite probiotic preparation can be used for preparing antioxidant products. The composite probiotic preparation disclosed by the invention consists of streptococcus thermophilus S709, lactobacillus paracasei L578 and lactobacillus helveticus L551, can effectively remove oxygen free radicals (DPPH, ABTS, superoxide anion free radicals and hydroxyl free radicals) in vivo and in vitro, effectively reduces the oxidative stress level of cells, improves the activity of SOD and GSH-PX and increases the antioxidant capacity of a matrix, is free from any toxic and side effects, and establishes a foundation for exploring the action mechanism of the probiotics in antioxidation and sleep regulation.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to a new application of a compound probiotic preparation in the field of anti-oxidation. Background technique [0002] Human health is directly related to sleep. Healthy and adequate sleep can remove metabolites and oxygen free radicals in the body, enhance immune function, consolidate memory, and protect the nervous system. Long-term sleep deprivation and insomnia caused by excessive stress can induce oxidative stress, causing excessive active oxygen free radicals in cell metabolism, weakening the antioxidant function system of the matrix, and causing various diseases. [0003] In order to remove oxygen free radicals in the body, reduce their damage to the body, and restore the health of the body, antioxidant drugs are often used to deal with oxygen free radicals. Existing antioxidant drugs are mainly enzyme system (antioxidant enzyme) drugs, including superoxide dismutase (SOD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A61K35/744A61K35/747A61P39/06C12N1/20C12R1/46C12R1/225
CPCA23L33/135C12N1/20A61K35/744A61K35/747A61P39/06A23V2002/00A23V2400/147A23V2400/249A23V2200/30
Inventor 杨玲齐世华孙策仵红岩路江浩鄢梦洁贾晓蒙贾洪利赵林森何方
Owner 河北一然生物科技股份有限公司
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