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Bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, application and drug loading system

A technology of outer membrane vesicles and nanoparticles, applied in the field of supramolecular chemistry, can solve the problems of rejection, affecting the activity and physiological functions of cell carriers, large-scale preparation of limited endogenous cell extraction, etc., to achieve the preparation process Simplicity, effects of inhibiting efflux and overcoming challenges

Pending Publication Date: 2022-01-04
UNIVERSITY OF MACAU
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current preparation of cell carrier preparations usually requires the extraction of endogenous cells first, and its large-scale preparation is limited by the amount of endogenous cells extracted, and then requires complex processing steps in vitro, which may affect the activity and Physiological function, and endogenous carrier cells only correspond to a single host individual, and rejection reactions will still occur when applied to other individuals

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  • Bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, application and drug loading system
  • Bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, application and drug loading system
  • Bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, application and drug loading system

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preparation example Construction

[0030] In the second aspect, an embodiment of the present invention provides a method for preparing the above-mentioned supramolecular nanoparticle precursor coated with bacterial outer membrane vesicles, which includes: respectively coating bacterial outer membrane vesicles with nanoparticles modified by macrocyclic host molecules and nanoparticles modified with corresponding guest molecules, and then mix the nanoparticles coated with bacterial outer membrane vesicles coated with host molecules and the nanoparticles coated with bacterial outer membrane vesicles coated with guest molecules to obtain bacterial outer membrane vesicles Bubble-coated supramolecular nanoparticle precursors.

[0031] In a preferred embodiment of the application of the present invention, the above-mentioned bacterial outer membrane vesicles are coated on the surface of nanoparticles by liposome extruder or ultrasonic treatment.

[0032] In a preferred embodiment of the application of the present inve...

Embodiment 1

[0043] This example provides a supramolecular nanoparticle precursor coated with bacterial outer membrane vesicles and its preparation method. In this example, the bacteria are Escherichia coli, and the gold nanoparticles are purchased from Xi'an Ruixi Biotechnology Co., Ltd., DMEM The medium was purchased from Thermo Fisher Scientific (China) Co., Ltd., and the mercapto-β-cyclodextrin and adamantanethiol were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0044] Gold nanoparticles were reacted with mercapto-β-cyclodextrin and adamantanethiol to prepare β-cyclodextrin-modified gold nanoparticles (such as figure 1 ) and adamantane-modified gold nanoparticles.

[0045] Collect Escherichia coli outer membrane vesicles, mix Escherichia coli outer membrane vesicles with β-cyclodextrin-modified gold nanoparticles or adamantane-modified gold nanoparticles at a mass ratio of 1:1, and use ultrasonic treatment to produce Coli outer membrane vesicles coated with gold...

Embodiment 2

[0049] This example provides a supramolecular nanoparticle precursor coated with bacterial outer membrane vesicles and its preparation method. In this example, the bacterium is Staphylococcus aureus, and the gold nanoparticles are purchased from Xi'an Ruixi Biotechnology Co., Ltd. , DMEM medium was purchased from Thermo Fisher Scientific (China) Co., Ltd., and mercapto-β-cyclodextrose and mercapto-ferrocene were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0050] The gold nanoparticles modified by β-cyclodextrin and the gold nanoparticles modified by ferrocene can be prepared by reacting gold nanoparticles with mercapto-β-cyclodextol and mercapto-ferrocene respectively.

[0051] The outer membrane vesicles of Staphylococcus aureus were collected, and the outer membrane vesicles of Staphylococcus aureus were mixed with gold nanoparticles modified with β-cyclodextrin or gold nanoparticles modified with ferrocene at a mass ratio of 5:1. Gold nanoparticles co...

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Abstract

The invention discloses a bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, application and a drug delivery system, and relates to the technical fields of supramolecular chemistry, supramolecular materials and cell preparations. Based on the bacterial outer membrane vesicle coated supramolecular nanoparticle precursor, in-vivo immune cells can be specifically recognized, and an intracellular nanoparticle aggregate mediated by host-guest interaction is further constructed in cells. The endogenous immune cell carrier can respond to the inflammatory characteristics of focus tissues (such as tumors), so that the convenient trolley type delivery of intracellular nanoparticle aggregates is realized, and the excretion phenomenon of immune cells is inhibited. The preparation method of the in-vivo (and intracellular) supramolecular nanoparticle assembly mediated by the host-guest interaction has the advantages of being simple in preparation process, rapid and universal, and challenges faced by the construction and in-vivo delivery process of a cell carrier preparation can be overcome. In addition, the invention further provides a drug loading system, and drug loading of targeted drug therapy can be achieved.

Description

technical field [0001] The invention relates to the technical fields of supramolecular chemistry, supramolecular materials and cell preparations, in particular to the supramolecular nanoparticle precursor, application and drug-carrying system coated with bacterial outer membrane vesicles. Background technique [0002] In recent years, the rapid development of bionic nanomedicine, especially the emerging field of cell carriers, provides a unique strategy for improving the biocompatibility of artificially synthesized preparations. However, the current preparation of cell carrier preparations usually requires the extraction of endogenous cells first, and its large-scale preparation is limited by the amount of endogenous cells extracted, and then requires complex processing steps in vitro, which may affect the activity and Physiological functions, and the endogenous carrier cells only correspond to a single host individual, and rejection reactions will still occur when applied t...

Claims

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Application Information

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IPC IPC(8): A61K47/69
CPCA61K47/54A61K47/6907A61K47/6903A61K47/6923A61K47/6911A61K47/6949A61K47/6951A61K47/6925A61K45/00A61P29/00A61P31/04A61P35/00A61P25/00B82Y5/00Y02A50/30
Inventor 王瑞兵高成李铭源王庆福
Owner UNIVERSITY OF MACAU
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