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Lactobacillus rhamnosus and application thereof

A technology of Lactobacillus rhamnosus and Helicobacter pylori, which is applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of gastric acid tolerance, poor colonization ability in the stomach, and difficult colonization, so as to inhibit inflammation and maintain microbiology. Effects of Ecological Balance, Good Gastric Mucosa Adhesion

Active Publication Date: 2021-12-24
SOUTHERN MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, Lactobacillus rhamnosus LGG has poor gastric acid tolerance and gastric colonization ability, and it is difficult to colonize gastric tissue to inhibit H.pylori

Method used

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  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof
  • Lactobacillus rhamnosus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Separation and identification of rats Laridum Laridin LRX-01

[0041] 1, 李 乳 Lactobacillus LRX-01 separation

[0042] Includes the following steps:

[0043] (1), collecting pure breastfeeding health new feces, adding PBS dilution in the ratio of solid-liquid ratio of 1: 1000 (g / ml);

[0044] (2), taking 5 μl of diluted samples coated with MRS solid medium, 37 ° C constant temperature anatomeric culture 48h;

[0045] (3) According to the colors, size, edge shape, etc., inoculate the colony, scribble purification;

[0046] (4) Picking a single colony, inoculation to MRS liquid medium, yty culture,

[0047] 2, morphological characteristics of strains

[0048] Colony characteristics: colonies are well grown, which are milky white, round, protrusions, and edges.

[0049] Bacterial characteristics: short rod, no exercise, non-spore, polyuretiogenic, Gram-stained positive (eg figure 1 Indicated.

[0050] 3, molecular biological identification of strains

[0051] Bacter...

Embodiment 2

[0065] Example 2 Test of the tolerance of Rats Lactobacillus LRX-01 of the present invention on artificial gastric fluid

[0066] Artificial gastric fluids having a pH of 2.0, 3.0, 4.0, respectively. Take the rat Li-supass Standard Strain ATCC53103 and the rats Lactobacillus LRX-01 of the present invention, diluted with a sterile PBS to 10 9 CFU / ml of bacterial suspension. In the artificial gastric liquid of 10 μl of the bacterial suspension at 990 μl of different pH, at 37 ° C for anaerobic incubation, the initial and cultured live bacteria were measured using a dilution drip method. The results are shown in Table 2.

[0067] Table 2 Comparison of Artificial Gastric Liquid Performance of ATCC53103 and LRX-01

[0068] Strain pH2 pH3 pH4 ATCC53103 1.30％±0.14％ 94.80％±4.09％ 96.48％±2.73％ LRX-01 2.09％±0.07％ 97.35％±2.51％ 97.37％±4.05％

[0069] It can be seen from Table 2, compared with the rats of Lactobacillus standard strain ATCC 53103, the rats of the p...

Embodiment 3

[0070] Example 3 Rats Lactobacillus LRX-01 Adhesive Turpoda LRX-01 Adhesive Turkacillus

[0071] Includes the following steps:

[0072](1), cell recovery and passage: Remove gastric epithelial cells from liquid nitrogen irrigation, rapidly placed in a water bath of 37 ° C until completely melted, sterile operation to absorb cell suspension to 15 ml of sterile centrifugal tube, The appropriate amount of GES-1 complete cell culture medium was slowly added, and 1000 rpm was centrifuged for 5 minutes. After discarding the supernatant, 5 ml complete medium was added, placed at 37 ° C, 5% CO. 2 Culture in the incubator; after the cell convergence rate of 80% -90%, the residual serum and medium were rinsed with sterile PBS, 1 ml of 0.25% EDTA-containing trypsin digestion cells were added, and the microscope was met in the microscope after several minutes. To fall off, 10 ml of complete culture solution was added to stop digestion, gently blown cells into a single cell with a dropper, tra...

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Abstract

The invention discloses a strain of lactobacillus rhamnosus LRX-01, and the preservation number of the strain LRX-01 is GDMCC No: 60986. The lactobacillus rhamnosum LRX-01 disclosed by the invention has the general characteristics of probiotics, and has the advantages of good gastric acid resistance and good gastric mucosa adhesiveness. The lactobacillus rhamnosus LRX-01 can inhibit adhesion and colonization of helicobacter pylori in gastrointestinal tracts, can effectively inhibit growth of the helicobacter pylori, inhibits inflammatory response, helps to repair gastric mucosa tissues, and maintains microecological balance of the gastrointestinal tracts.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and in particular, there is a lactobacillusrhamnosus LRX-01, which is lactobacter, lactobacter, and its application. Background technique [0002] Helicobacter pylori, h.pylori is a Gram-negative bacterium, mainly survive in human stomach and twelve colon, which is caused active gastritis, peptic ulcer, gastric cancer, gastric mucosa-related lymphatic tissue lymphoma One of the important causes of digestive system disease has been confirmed by the World Health Organization International Cancer Research Institute as Class I caused carcinogenic factors. The recent results show that Helicobacter may also be associated with cardiovascular, skin, nerve, immunization, blood, hepatobiliary, breathing, endocrine and metabolic disorders. Effective intervention and control of H.Pylori infection, thereby preventing gastric cancer and reducing the incidence of other H. Pylori-associated diseases is already a ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P1/04A61P31/04A61P35/00A23L33/135A23C9/123A23K10/18C12R1/225
CPCA61K35/747A61P31/04A61P1/00A61P1/04A61P35/00A23L33/135A23C9/1234A23K10/18A23V2002/00A23V2400/175A23V2200/3204Y02A50/30
Inventor 范宏英陈振辉白杨曾位森彭运平孟晓静
Owner SOUTHERN MEDICAL UNIVERSITY
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