Additive and feed for improving anti-stress capability of penaeus vannamei boone and application of additive and feed
A white shrimp, anti-stress technology, applied in the application, animal feed, animal feed and other directions, to prevent abnormal deposition in the hepatopancreas, has wide application prospects, and is convenient for large-scale promotion.
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Embodiment 1
[0033] Example 1 Effect of vitamin C content on growth performance and antioxidant level of Penaeus vannamei
[0034] 1.1 Experimental method
[0035] 1.1.1 Experimental group settings
[0036] Vitamin C polyphosphate (35% effective content) was used as the source of vitamin C in the test, and the concentration of vitamin C in the feed of the test group was determined by high performance liquid chromatography.
[0037] Test group 1:
[0038] Feed additive: The mass ratio of vitamin C (measured content) to myo-inositol (added amount) is: 48.55:300
[0039] Feed formula: 25% fish meal, 25% peeled soybean meal, 12% peanut bran, 24.27% wheat flour, 5% krill meal, 3% brewer's yeast, 1% fish oil, 1% soybean oil, 1% soybean lecithin; Calcium hydrogen 1%, choline chloride (effective content 50%) 0.2%, compound mineral salt 0.5%, multivitamin 1% (the effective content of myo-inositol is 97%, the added amount is 300mg / kg feed), vitamin C Polyphosphate 0.03%. The vitamin C content i...
Embodiment 2
[0066] Example 2 Effects of inositol content on the ability of Penaeus vannamei to resist salinity stress, hypoxia stress and ammonia nitrogen stress and antioxidant level
[0067] 2.1 Experimental method
[0068] 2.1.1 Test group settings
[0069] Test group 5
[0070] Feed additive: the mass ratio of myo-inositol (added amount) to vitamin C (added amount) is: 100:250
[0071] Feed formula: fish meal 20%, peeled soybean meal 25%, peanut bran 12%, wheat flour 24.29%, krill meal 5%, brewer's yeast 3%, soybean protein concentrate 4%, fish oil 2%, soybean oil 2%, diphosphate Calcium hydrogen 1%, choline chloride (effective content 50%) 0.2%, compound mineral salt 0.5%, multivitamin 1% (the amount of vitamin C added is 250mg / kg feed), myo-inositol (effective content 97%) The added amount is 100mg / kg feed. The content of myo-inositol in the feed obtained by gas chromatography (NY / T 1345-2007) is 368.8mg / kg feed.
[0072] Test group 6
[0073] Feed additive: the mass ratio of ...
Embodiment 3
[0123] Example 3 Effects of Vitamin C and Inositol on Oxidative Stress Caused by High Copper
[0124] 3.1 Experimental method
[0125] 3.1.1 Test group settings
[0126] Test group 10
[0127] Feed additive (addition amount): the mass ratio of myo-inositol, vitamin C and copper sulfate pentahydrate is: 200:250:430.76.
[0128] Feed formula: fish meal 20%, peeled soybean meal 25%, peanut bran 12%, wheat flour 24.04%, krill meal 5%, brewer's yeast 3%, soybean protein concentrate 4%, fish oil 2%, soybean oil 2%, diphosphate Calcium hydrogen 1%, choline chloride (effective content 50%) 0.2%, compound mineral salt 0.7%, multivitamin 1% (the amount of vitamin C added is 250mg / kg feed), myo-inositol (effective content 97%) The addition amount is 200mg / kg feed, and the addition amount of copper sulfate pentahydrate is 430.76mg / kg feed. The content of myo-inositol in the feed determined by gas chromatography (NY / T 1345-2007) is 465.9 mg / kg feed; the content of copper in the feed me...
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