Slow-release carbon source immobilized aerobic denitrification complex microbial agent, and preparation method and application thereof

A nitrifying composite bacterial agent and aerobic denitrification technology, applied in biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the problem of being easily washed away by running water and the efficiency of denitrification Low-level problems, to achieve the effect of improving the plant rhizosphere microbial community, large specific surface area, and long service life

Active Publication Date: 2021-11-26
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The composite bacterial agent of the present invention can solve the technical defect of the existing denitrification bacterial agent in the prior art, which is easily washed away by running water and causes low denitrification efficiency

Method used

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  • Slow-release carbon source immobilized aerobic denitrification complex microbial agent, and preparation method and application thereof
  • Slow-release carbon source immobilized aerobic denitrification complex microbial agent, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment

[0030] I. Preparation of inocula

[0031] Aerobic denitrifying bacteria selected for preservation laboratory denitrifying agents Pseudomonas mendocina LYX and Acidovorax sp.strain YD725, strains were deposited at China General Microbiological Culture Collection Center CGMCC, under the accession number CGMCC No.10611, China Center for Type culture Collection under accession number CCTCC NO: M2021536. Both strains placed before the experiment were cultured in LB medium until the logarithmic phase, logarithmic phase denitrifying bacteria liquid in the rotation speed 8000 r / min centrifugal 10min, the supernatant was poured off, leaving the bacterial pellet the two strains according to precipitate 1: 1 ratio, with 0.9% saline to form a composite volume to 5mL broths.

[0032] The shape itself according loofah cut 1cm thickness of the block body, placed in a 4% NaOH solution was boiled 30min, then rinsed with deionized water to remove residual NaOH solution, and then placed in an oven ...

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Abstract

The invention discloses a slow-release carbon source immobilized aerobic denitrification complex microbial agent, and a preparation method and application thereof. The slow-release carbon source immobilized aerobic denitrification complex microbial agent is prepared from a complex microbial agent and retinervus luffae fructus used for immobilizing the complex microbial agent, wherein the nitrate nitrogen removal rate of the complex microbial agent is not lower than 96%. According to the invention, the technical defect of low denitrification efficiency caused by the fact that a denitrification microbial agent in the prior art is likely to be washed away by flowing water is overcome through the immobilized aerobic denitrification complex microbial agent. In addition, an aerobic denitrification technology and a plant treatment technology are combined for use, a microorganism-plant remediation system based on the complex microbial agent is constructed, plant rhizosphere microbial communities are improved, a plant treatment effect is enhanced, the enhancement effect of plants for nitrogen pollution removal is further achieved, and deep treatment of drinking water pollution is achieved.

Description

Technical field [0001] The present invention relates to the field of environmental biotechnology, particularly to a sustained release carbon immobilized denitrifying composite microbial agent and its preparation and use. Background technique [0002] In recent years, with the development of the social economy, industrial and agricultural waste water discharged into water bodies constantly, causing serious nitrate pollution, contaminated drinking water is a major problem facing. Nitrate pollution not only causes serious deterioration of eutrophication and other water quality, seriously affecting the quality of drinking water. While also converting nitrate to nitrite in the body, the direct oxidation of hemoglobin to methemoglobin, leading to serious human tissue hypoxia, affect human health and the survival of amphibians. Water pollution control plant as a conventional water treatment technology, is widely used, [0003] Denitrifying technique can remove carbon and nitrogen simult...

Claims

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Application Information

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IPC IPC(8): C12N11/02C12N1/20C02F3/32C02F3/10C02F3/34C12R1/38C12R1/01C02F101/16
CPCC12N11/02C12N1/20C02F3/32C02F3/10C02F3/348C02F3/109C02F2101/163Y02W10/10
Inventor 许燕滨杨德江进李宁
Owner GUANGDONG UNIV OF TECH
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