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Crude sargassum pallidum polysaccharide, and preparation method, separation and purification method and application thereof

A technique for separation and purification of Artemisia japonica polysaccharide, which is applied in the application, polysaccharide/gum-containing food ingredients, food ingredients, etc., can solve the problems affecting the wide application of Artemisia japonica polysaccharide, low extraction rate, etc., to improve macrophages and spleen lymphocytes. Cell proliferation, high oxidative activity, and the effect of promoting cytokine production

Pending Publication Date: 2021-11-09
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low extraction rate of crude polysaccharides from Artemisia chinensis, and the crude polysaccharides contain many components, the wide application of polysaccharides in medicine has been seriously affected.

Method used

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  • Crude sargassum pallidum polysaccharide, and preparation method, separation and purification method and application thereof
  • Crude sargassum pallidum polysaccharide, and preparation method, separation and purification method and application thereof
  • Crude sargassum pallidum polysaccharide, and preparation method, separation and purification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Preparation of artemisiase crude polysaccharide

[0059] The artemisiase algae was pulverized, over 100 mesh sieve to give algal powder. The algae powder is extracted with hot water soaking method, and the liquid ratio is 1:30 (g / ml), 91 ° C is extracted for 5 h, and the mixture after leaching is centrifuged (2000 r / min, 15min), abandoned the precipitate, take The supernatant was evaporated to the original volume of 1 / 4, and then the crystal molecular weight was dialyzed with a dialysis bag of 3500 kD, and the inorganic salt small molecular material was removed. Then vaporly evaporated, concentrated to 1 / 4 of the dialysis solution, then add 4x volume anhydrous ethanol for alcohol, 4 ° C overnight, then centrifuged (7000 r / min, 15min), freeze and dry The crude polysaccharide was obtained, and the calculated rate was 6.74%.

Embodiment 2

[0061] Isolation and purification of artemisiac-crude polysaccharides

[0062] (1) Elution separation of artemison crude polysaccharide

[0063] It is suggested that the artememon crude polysaccharide prepared by 1 g of Example 1 was dissolved in 10 ml of distilled water, and 7.5 ml of SEVAG reagent (according to volume ratio, n-butanol: chloroform = 1: 4 mixed solution), sufficient shock mix, The supernatant was separated by centrifugation (7000 R / min, 15min), ie a polysaccharide solution. The polysaccharide solution was sequentially washed with a filtered 0m, 0.1 m, 0.3m, 0.5m, 0.7 m, 1.3m, 0.5m, 0.7m, 1.3m, 1.5m, 1.7m, 2.0m, sodium chloride solution. Take off, the elution volume of each concentration of sodium chloride solution is 2L, collecting 10ml using the automatic collector, and 10 ml of the phenol-sulfuric acid method, the absorbance is measured at 485 nm, and the elution curve is drawn according to the absorbance. (like figure 1 Indicated. 5 components of the polysacc...

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Abstract

The invention discloses a crude sargassum pallidum polysaccharide and a preparation method thereof. The crude sargassum pallidum polysaccharide is extracted by taking sargassum pallidum frond as a raw material, and monosaccharide comprises galactose, fucose and mannose, and structurally has typical S=O symmetrical stretching vibration peaks and alpha-configuration glucosidic bonds. The preparation method of thecrude sargassum pallidum polysaccharide comprises the following steps: performing hot water extraction, concentration, dialysis, alcohol precipitation and freeze drying on sargassum pallidum frond to obtain the crude sargassum pallidum polysaccharide. The invention also discloses a separation and purification method of the crude polysaccharide. The separation and purification method comprises the following steps: deproteinizing the crude polysaccharide through a Sevag reagent, eluting and separating through a DEAE-cellulose anion exchange column, and then concentrating, dialyzing, carrying out alcohol precipitation and drying to obtain powder of each component of the sargassum pallidum polysaccharide. The sargassum pallidum polysaccharide obtained by the invention has the effects of improving the immunity of the organism and inhibiting the growth of tumor cells, and can be applied to healthy foods and / or pharmaceutical preparations for adjuvant therapy of tumors or improvement of the immune regulation ability of the organism.

Description

Technical field [0001] BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the field of extraction and purification of natural products, and therefore, there is an artemisiac crude polysaccharide and a method of preparing and separation and purification. Background technique [0002] Artemisiac is a seaweed in the coastal area, which is a seaweed, which is multi-long on the rock. Artemisiac is one of the traditional Chinese herbal algae, which has a high medicinal value and development potential. Pharmacological studies have shown that polysaccharides are one of the main active substances of artemissence. However, due to the low extraction rate of artemisiase crude polysaccharide, and the crude polysaccharide contains more components, it has seriously affected artemisiac polysaccharides in medical interaction. Therefore, the preparation method of artememisiac-crude polysaccharide with high extraction rate is provided and its further separation a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P37/04A23L33/125
CPCC08B37/006C08B37/0003A61K31/715A61P35/00A61P37/04A23L33/125A23V2002/00A23V2200/308A23V2200/324A23V2250/51
Inventor 高羿牛蕴泽李溢真宋琳鞠豪陈冉
Owner QINGDAO AGRI UNIV
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