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Armeniaca vulgaris lam pectin polysaccharide and preparation method thereof

A technology of pectin polysaccharide and apricot fruit, which is applied in the field of apricot pectin polysaccharide and its preparation, can solve the problems of large molecular weight of pectin polysaccharide, large difference in polysaccharide yield, insoluble and other problems, so as to improve the extraction amount and purity, and benefit The effect of mass application and reduced withdrawal fee

Active Publication Date: 2021-11-02
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different extraction processes have their own advantages and disadvantages, and the yield of polysaccharides also varies greatly
The hot water extraction method takes a long time and the polysaccharide extraction rate is low; although the enzyme extraction method can obtain a higher polysaccharide yield, the high cost of the enzyme limits its wide application; the ultrasonic extraction method is extracted in hot water. On the basis of adding ultrasonic extraction steps, the extraction yield is relatively high, so it has a relative advantage
The development of pectin polysaccharides is limited due to its large molecular weight and insoluble

Method used

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  • Armeniaca vulgaris lam pectin polysaccharide and preparation method thereof
  • Armeniaca vulgaris lam pectin polysaccharide and preparation method thereof
  • Armeniaca vulgaris lam pectin polysaccharide and preparation method thereof

Examples

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Effect test

preparation example Construction

[0034] b Preparation of spore solution

[0035] Wash the spores into a 150mL Erlenmeyer flask with sterile water, and adjust the concentration to 1.55×10 7 individual / mL.

[0036] c Preparation of fermentation medium

[0037] Weigh 10g of apricot pulp without active water homogenization and add 50mL of distilled water in a 250mL Erlenmeyer flask, inoculate the Aspergillus niger spore liquid into the fermentation medium according to the inoculum size of 6%, and the other components of the medium are potassium dihydrogen phosphate 3g / L, magnesium sulfate 1.5g / L, CMC-Na 10g / L, peptone 0.5g / L, adjust the pH value to 6.0, 121°C, 15min for later use, and incubate at 140rpm for 5 days to obtain the fermentation enzyme liquid.

Embodiment 1

[0038] Embodiment 1 microbial fermentation method prepares apricot pectin polysaccharide

[0039] Preparation of apricot pulp: use low-temperature plasma jet discharge mode, under the power of 350w, process 500mL volume of distilled water for 30s. Mix and homogenate according to the ratio of solid to liquid (w / v, g / mL) 1:1.

[0040] Weigh 10g of apricot pulp and add distilled water into the Erlenmeyer flask according to the ratio of solid-liquid ratio (w / v, g / mL) 1:10, adjust the pH value to 6.0, add the Aspergillus niger fermentation enzyme liquid according to the amount of 20% of the mass of the apricot pulp , after mixing, incubate at 40° C. and 120 rpm for 6 hours to obtain an enzymatic hydrolysis solution.

[0041]Extract polysaccharide: Inactivate the enzyme at 90°C for 10 minutes, centrifuge, take the supernatant, and dilute to 100mL. Concentrate to 1 / 2 the volume with a rotary evaporator at 45°C, ethanol precipitation with 3 times the volume of absolute ethanol, plac...

Embodiment 2

[0042] Example 2 Preparation of Apricot Pectin Polysaccharide by Microbial Fermentation

[0043] Preparation of apricot pulp: use low temperature plasma jet discharge mode, under the power of 400w, process 500mL volume of distilled water for 60s. Mix and homogenate according to the ratio of material to liquid (w / v, g / mL) 1:2.

[0044] Weigh 10g of apricot pulp and add distilled water into the Erlenmeyer flask according to the ratio of solid-liquid ratio (w / v, g / mL) 1:10, adjust the pH value to 6.0, and add Aspergillus niger fermentation enzyme liquid in an amount of 30% of the mass of the apricot pulp , after mixing, incubate at 50°C and 100rpm for 8h to obtain enzymatic hydrolysis solution.

[0045] Extraction of polysaccharides: Inactivate the enzyme at 90°C for 10 minutes, centrifuge, take the supernatant, and dilute to a certain volume. Concentrate to 1 / 3 volume with a rotary evaporator at 45°C, ethanol precipitation with 4 times the volume of absolute ethanol, place at ...

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Abstract

The invention provides an armeniaca vulgaris lam pectin polysaccharide and a preparation method thereof. The method comprises: pulping armeniaca vulgaris lam fruits and active water according to a material-liquid ratio of 1:(1-3) to obtain pulp, wherein the material-liquid ratio is a mass-volume ratio, and the unit is g / mL; and carrying out mixing enzymolysis on the pulp and an aspergillus niger fermentation broth, carrying out centrifugal concentration 2-4 times after enzymolysis is finished, adding absolute ethyl alcohol with the volume 2-5 times, carrying out alcohol precipitation, and freeze drying for 12-24 h to obtain the armeniaca vulgaris lam pectin polysaccharide. According to the invention, the extraction step of the natural plant polysaccharide is greatly simplified, and the aspergillus niger strain which can be separated and extracted from the nature is utilized, so that the extraction cost is reduced to a great extent, the extraction amount and purity of the natural plant polysaccharide are improved, toxic and harmful reagents such as diethyl ether and chloroform are prevented from being used, and large-scale application of the natural plant polysaccharide is facilitated.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to an apricot pectin polysaccharide and a preparation method thereof. Background technique [0002] Apricot is a Rosaceae plant (Armeniaca vulgaris Lam). It is sweet, sour, flat in nature, slightly warm, and has the effects of promoting body fluid and quenching thirst, moistening lungs and relieving asthma. Apricot not only has the characteristics of general fruits, but also has a good prospect of development and utilization in medicinal aspects: apricot is suitable for lowering blood fat, acute and chronic bronchitis cough, lung cancer, nasopharyngeal cancer, breast cancer patients and their radiation and chemotherapy. Domestic and foreign research on apricots mostly focuses on the biological activity of amygdalin in almonds, and there are relatively few research reports on polysaccharides in apricot pulp. Fruit polysaccharides generally have immune regulation, anti-tumor, anti-oxidati...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 吴彩娥孙文娟索安迪范龚健李婷婷李晓静周丹丹
Owner NANJING FORESTRY UNIV
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