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3D printing hydrogel for treating or preventing intrauterine adhesion and preparation method of hydrogel

A 3D printing, intrauterine adhesion technology, applied in medical science, surgery, additive processing, etc., can solve the problem of reduced cell proliferation rate and cell viability, single structure of pure collagen hydrogel, poor mechanical properties and rapid degradation and other problems, to achieve the effects of controllable degradability, high cell encapsulation efficiency, and excellent biocompatibility

Active Publication Date: 2021-10-29
广州达康基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing 3D printed hydrogels use ultraviolet light to initiate cross-linking, however, ultraviolet cross-linking can lead to a decrease in cell proliferation rate and cell viability
Meanwhile, although hydrogels based on natural materials such as chitosan, gelatin, and hyaluronic acid have been used to prevent adhesion, satisfactory therapeutic effects have not yet been obtained due to their poor mechanical properties and rapid degradation.
In addition, collagen is the main component of the extracellular matrix (ECM), however, pure collagen hydrogels have a single structure, simple properties, and poor mechanical properties, which in turn limit their applications.

Method used

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  • 3D printing hydrogel for treating or preventing intrauterine adhesion and preparation method of hydrogel
  • 3D printing hydrogel for treating or preventing intrauterine adhesion and preparation method of hydrogel
  • 3D printing hydrogel for treating or preventing intrauterine adhesion and preparation method of hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Preparation of methacrylated gelatin (GelMA): 10 g of gelatin was dissolved in 100 mL of deionized water at 50° C. to prepare a gelatin solution. Then 6 mL of methacrylic anhydride was added dropwise to the gelatin solution, and the reaction was stirred for 6 h. Dialyze with distilled water for 3-5 days in a dialysis bag (molecular weight cut-off: 12-14 kDa) to remove impurities and unreacted methacrylic anhydride. Finally, it was freeze-dried and stored in the dark at -80°C until use.

Embodiment 2

[0035] Preparation of methacrylated collagen (ColMA): 2 g of collagen was dissolved in 100 mL of deionized water at 37° C. to prepare a collagen solution. Then, 1.2 mL of methacrylic anhydride was added dropwise to the collagen solution, stirred for 12 hours, and the pH value of the reaction system was kept at 8-9 by using NaOH with a concentration of 5 mol / L. Dialyze with distilled water in a dialysis bag (molecular weight cut-off: 12-14kDa) for 3-5 days, then freeze-dry the final dialysis product, and store it in the dark at -20°C until use.

Embodiment 3

[0037] A preparation method for 3D printing hydrogel, the steps are as follows:

[0038] (1) Preparation of printing ink: the methacrylated gelatin prepared in Example 1, the methacrylated collagen prepared in Example 2, and the LAP photoinitiator were added to deionized water, and mixed evenly to obtain a printing ink, which was stored until Wherein, by the volume of deionized water, the addition amount of methacrylated gelatin is 5% g / mL, the addition amount of methacrylated collagen is 0.5% g / mL, the addition amount of LAP photoinitiator 0.01% g / mL;

[0039] (2) Design and load the 3D model: use 3DS Max software to design the model, import the model into the 3D printer control software, the model is a layer-by-layer lattice structure, and the specification is 16mm×16mm×2 layers;

[0040] (3) 3D printing preparation: transfer the printing ink prepared in step (1) to the extrusion cylinder of the extruded bio-3D printer, set the fiber diameter to 100 μm, the printing speed t...

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Abstract

The invention discloses a preparation method of 3D printing hydrogel for treating or preventing intrauterine adhesion. The method comprises the following steps: adding methacrylic acid gelatin, methacrylic acid collagen and a photoinitiator into deionized water, and uniformly mixing to obtain printing ink; designing a model by using 3DS Max software, and importing the model into 3D printer control software; transferring printing ink and human amniotic mesenchymal stem cells into an extrusion cylinder of a 3D printer, printing to obtain a primary hydrogel product, and irradiating and curing the primary hydrogel product with blue light to obtain the 3D printing hydrogel for treating or preventing intrauterine adhesion. The 3D printing hydrogel disclosed by the invention has excellent biocompatibility and in-situ crosslinking capability, controllable mechanical property, swelling property and degradability and high cell encapsulation efficiency, hAMSC can be loaded inside the hydrogel, controllable release of hAMSC can be realized, hAMSC can be continuously released in vitro for more than 7 days, and intrauterine adhesion can be effectively treated or prevented by utilizing hAMSC.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a 3D printing hydrogel for treating or preventing intrauterine adhesions and a preparation method thereof. Background technique [0002] Intrauterine adhesions (IUA), also known as Asherman syndrome, refer to endometrial basal layer damage caused by induced abortion, curettage and other factors, resulting in partial or complete adhesion of the uterine cavity and cervical canal. The clinical manifestations of IUA are mainly hypomenorrhea, recurrent miscarriage and amenorrhea, which seriously damage the reproductive health of women. [0003] With the development of hysteroscopic technology, hysteroscopic electrosurgical resection has the advantages of minimally invasive, safe and effective treatment, and has become the preferred choice for the treatment of IUA. However, the recurrence rate of secondary IUA due to postoperative wound exudation and infection...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00B33Y70/00A61L31/00A61L31/04A61L31/14A61L31/16
CPCC08J3/075B33Y70/00A61L31/045A61L31/044A61L31/005A61L31/145A61L31/14A61L31/148A61L31/16C08J2389/00C08J2489/00A61L2300/602A61L2300/41
Inventor 肖扬黄建波陈丽璇郭瑞胡圣学
Owner 广州达康基因技术有限公司
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