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Degradable super-tough silk protein-based material and preparation method and application thereof

A silk protein, protein-based technology, applied in the direction of single-component silk fibroin artificial filament, rayon manufacturing, textile and paper making, etc. Elongation and toughness, the effect of a wide range of raw materials

Active Publication Date: 2021-10-22
WESTLAKE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some progress has been made through previous related research, until now, there is still a lack of very effective and easy processing and preparation methods

Method used

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  • Degradable super-tough silk protein-based material and preparation method and application thereof
  • Degradable super-tough silk protein-based material and preparation method and application thereof
  • Degradable super-tough silk protein-based material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0056] Prepare the degradable super tenacious fibroin film according to the following method:

[0057] (1) As in Table 1, prepare silk protein solution, glycerin solution, and calcium chloride solution respectively, and mix the above-mentioned solutions according to the volumes in Table 1 after the preparation is completed. Take 3.5mL of the mixed solution and pour it into a petri dish with a diameter of 6mm, so that the solution completely covers the surface of the petri dish.

[0058] (2) The petri dish poured with the mixed solution was left open and dried at room temperature for 12 hours, and then the dried petri dish was placed in a vacuum drying oven and continued to dry for 2-4 hours at room temperature to prepare each silk protein film ( 15% glycerin + 5% calcium chloride; 15% glycerin + 10% calcium chloride; 20% glycerin + 5% calcium chloride; 20% glycerin + 10% calcium chloride).

[0059] The photo of the prepared silk protein film (20% glycerol+10% calcium chloride...

Embodiment 3

[0079] The degradation performance of the film obtained in Example 3 was characterized by the degradation performance in different enzyme solutions (protease XIV, collagenase and α-chymotrypsin). Cut the samples into 10mm×10mm squares and soak them in 2mL of different enzyme solutions. The remaining samples were weighed on the first day and the third day respectively, and the degradation rate of the samples in different enzyme solutions could be calculated. The results of the enzymatic degradation test were as Figure 6 shown.

[0080] Analysis of results: from figure 2 and image 3 From the data in , it can be seen that with the increase of the proportion of added glycerol, the β-sheet content in the degradable super tenacious silk protein film also increases, and the glass transition temperature of silk protein molecules decreases.

[0081] From Figure 4 It can be seen from the data in that, as the ratio of glycerin and calcium chloride increases, the elongation at br...

Embodiment 5

[0089] Concentrate the low-concentration silk protein / glycerin / calcium chloride mixed aqueous solution prepared in Example 3 to prepare a high-concentration mixed silk protein aqueous solution. After the concentrated silk protein aqueous solution is centrifuged and purified, it can be stored stably at low temperature, and the concentration is 40%. Figure 7 It is a picture of high-concentration silk protein / glycerin / calcium chloride concentrate.

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Abstract

The invention relates to a degradable super-tough silk protein-based material and a preparation method and application thereof. The degradable super-tough silk protein-based material comprises a plasticizer, salt and silk protein, the plasticizer is selected from one or more of glycerin, sorbitol and polyethylene glycol, and the salt is selected from one or more of calcium chloride, lithium chloride and lithium bromide. By adjusting the ratio of the plasticizer to the salt in the super-tough silk protein-based material, the super-tough silk protein-based material has adjustable mechanical properties, degradation properties and water solubility, and can be developed into raw materials of various biological engineering materials depending on specific application.

Description

technical field [0001] The invention relates to a silk protein-based material, in particular to a degradable super-tough silk protein material, a preparation method and application thereof. Background technique [0002] Silk protein is a protein-based biopolymer extracted from natural silkworm cocoons, which has excellent mechanical properties, biocompatibility and biodegradability. In recent years, silk protein-based materials have been widely used in biomedical fields such as tissue engineering, bioelectronics, and regenerative medicine. For example, silk protein films can be used as substrates for the preparation of biosensors; silk protein sponges can be used as tissue engineering scaffolds; silk protein nanospheres can be used as carriers for drug delivery and release; silk protein blocks can be processed into implantable bone nails Wait. Usually, silk protein can be extracted from natural silkworm cocoons by dissolving and regenerating. This preparation method is gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08K3/16C08K5/053C08J5/18D01F4/02D01F1/10
CPCC08K3/16C08K5/053C08J5/18D01F4/02D01F1/10C08J2389/00C08K2003/162C08L89/00
Inventor 郭成辰吕浩
Owner WESTLAKE UNIV
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